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NMR assignment of the hypothetical ENTH-VHS domain At3g16270 from Arabidopsis thaliana
Authors
Lopez-Mendez, B., Pantoja-Uceda, D., Tomizawa, T., Koshiba, S., Kigawa, T., Shirouzu, M., Terada, T., Inoue, M., Yabuki, T., Aoki, M., Seki, E., Matsuda, T., Hirota, H., Yoshida, M., Tanaka, A., Osanai, T., Seki, M., Shinozaki, K., Yokoyama, S., Guntert, P.
Assembly
enth-vhs domain
Entity
1. enth-vhs domain (polymer, Thiol state: all free), 140 monomers, 15557.35 Da Detail

GSSGSSGESY WRSRMIDAVT SDEDKVAPVY KLEEICDLLR SSHVSIVKEF SEFILKRLDN KSPIVKQKAL RLIKYAVGKS GSEFRREMQR NSVAVRNLFH YKGHPDPLKG DALNKAVRET AHETISAIFS EENGSGPSSG


Formula weight
15557.35 Da
Source organism
Arabidopsis thaliana
Exptl. method
NMR
Refine. method
TORSION ANGLE DYNAMICS
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 95.7 %, Completeness: 93.9 %, Completeness (bb): 93.6 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All93.9 % (1515 of 1613)93.5 % (790 of 845)95.0 % (594 of 625)91.6 % (131 of 143)
Backbone93.6 % (777 of 830)93.3 % (266 of 285)94.4 % (387 of 410)91.9 % (124 of 135)
Sidechain94.4 % (862 of 913)93.6 % (524 of 560)95.9 % (331 of 345)87.5 % (7 of 8)
Aromatic90.9 % (100 of 110)90.9 % (50 of 55)90.7 % (49 of 54)100.0 % (1 of 1)
Methyl100.0 % (136 of 136)100.0 % (68 of 68)100.0 % (68 of 68)

1. enth-vhs domain

GSSGSSGESY WRSRMIDAVT SDEDKVAPVY KLEEICDLLR SSHVSIVKEF SEFILKRLDN KSPIVKQKAL RLIKYAVGKS GSEFRREMQR NSVAVRNLFH YKGHPDPLKG DALNKAVRET AHETISAIFS EENGSGPSSG

Sample

Temperature 298 (±0.5) K, pH 7.5 (±0.2)


#NameIsotope labelingTypeConcentration
1enth-vhs domain[U-13C; U-15N]1.20 mM
2Tris buffer20 mM
3NaCl100 mM
4dithiothreitol1 mM
5sodium azide0.02 % v/v
6H2O90 % v/v
7D2O10 % v/v

LACS Plot; CA
Referencing offset: 1.95 ppm, Outliers: 2 Detail
LACS Plot; CB
Referencing offset: 1.95 ppm, Outliers: 2 Detail
LACS Plot; HA
Referencing offset: 0.08 ppm, Outliers: 1 Detail
LACS Plot; CO
Referencing offset: 1.38 ppm, Outliers: 2 Detail
Protein Blocks Logo
Calculated from 20 models in PDB: 1VDY, Strand ID: A Detail


Release date
2004-04-06
Citation
NMR assignment of the hypothetical ENTH-VHS domain At3g16270 from Arabidopsis thaliana
Lopez-Mendez, B., Pantoja-Uceda, D., Tomizawa, T., Koshiba, S., Kigawa, T., Shirouzu, M., Terada, T., Inoue, M., Yabuki, T., Aoki, M., Seki, E., Matsuda, T., Hirota, H., Yoshida, M., Tanaka, A., Osanai, T., Seki, M., Shinozaki, K., Yokoyama, S., Guntert, P.
J. Biomol. NMR (2004), 29, 205-206, PubMed 15014234 , DOI 10.1023/B:JNMR.0000019239.44783.66 ,
Related entities 1. enth-vhs domain, : 1 : 1 : 21 entities Detail
Interaction partners 1. enth-vhs domain, : 1 interactors Detail
Experiments performed 16 experiments Detail
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