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BMRB: 5928

2DCP

NMR assignment of the hypothetical ENTH-VHS domain At3g16270 from Arabidopsis thaliana

Authors

Lopez-Mendez, B., Pantoja-Uceda, D., Tomizawa, T., Koshiba, S., Kigawa, T., Shirouzu, M., Terada, T., Inoue, M., Yabuki, T., Aoki, M., Seki, E., Matsuda, T., Hirota, H., Yoshida, M., Tanaka, A., Osanai, T., Seki, M., Shinozaki, K., Yokoyama, S., Guntert, P.

Assembly

enth-vhs domain

Entity

1. enth-vhs domain (polymer, Thiol state: all free), 140 monomers, 15557.35 Da Detail

GSSGSSGESY WRSRMIDAVT SDEDKVAPVY KLEEICDLLR SSHVSIVKEF SEFILKRLDN KSPIVKQKAL RLIKYAVGKS GSEFRREMQR NSVAVRNLFH YKGHPDPLKG DALNKAVRET AHETISAIFS EENGSGPSSG

Formula weight

15557.35 Da

Source organism

Arabidopsis thaliana

Exptl. method

NMR

Refine. method

TORSION ANGLE DYNAMICS

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.7 %, Completeness: 93.9 %, Completeness (bb): 93.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	93.9 % (1515 of 1613)	93.5 % (790 of 845)	95.0 % (594 of 625)	91.6 % (131 of 143)
Backbone	93.6 % (777 of 830)	93.3 % (266 of 285)	94.4 % (387 of 410)	91.9 % (124 of 135)
Sidechain	94.4 % (862 of 913)	93.6 % (524 of 560)	95.9 % (331 of 345)	87.5 % (7 of 8)
Aromatic	90.9 % (100 of 110)	90.9 % (50 of 55)	90.7 % (49 of 54)	100.0 % (1 of 1)
Methyl	100.0 % (136 of 136)	100.0 % (68 of 68)	100.0 % (68 of 68)

1. enth-vhs domain

GSSGSSGESY WRSRMIDAVT SDEDKVAPVY KLEEICDLLR SSHVSIVKEF SEFILKRLDN KSPIVKQKAL RLIKYAVGKS GSEFRREMQR NSVAVRNLFH YKGHPDPLKG DALNKAVRET AHETISAIFS EENGSGPSSG

Show sample condition

Sample

Temperature 298 (±0.5) K, pH 7.5 (±0.2)

#	Name	Isotope labeling	Concentration
1	enth-vhs domain	[U-13C; U-15N]	1.20 mM
2	Tris buffer		20 mM
3	NaCl		100 mM
4	dithiothreitol		1 mM
5	sodium azide		0.02 % v/v
6	H2O		90 % v/v
7	D2O		10 % v/v

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
¹H	H2O	protons	4.7 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Referencing offset: 1.95 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
¹H	H2O	protons	4.7 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Referencing offset: 1.95 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
¹H	H2O	protons	4.7 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Referencing offset: 0.08 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
¹H	H2O	protons	4.7 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Referencing offset: 1.38 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 1VDY, Strand ID: A Detail

Release date

2004-04-06

Citation

NMR assignment of the hypothetical ENTH-VHS domain At3g16270 from Arabidopsis thaliana
Lopez-Mendez, B., Pantoja-Uceda, D., Tomizawa, T., Koshiba, S., Kigawa, T., Shirouzu, M., Terada, T., Inoue, M., Yabuki, T., Aoki, M., Seki, E., Matsuda, T., Hirota, H., Yoshida, M., Tanaka, A., Osanai, T., Seki, M., Shinozaki, K., Yokoyama, S., Guntert, P.
J. Biomol. NMR (2004), 29, 205-206, PubMed 15014234 , DOI 10.1023/B:JNMR.0000019239.44783.66 , Abstract

Related entities 1. enth-vhs domain, : 1 : 1 : 21 entities Detail

Interaction partners 1. enth-vhs domain, : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Experiments performed 16 experiments Detail

1 2D [1H,15N ]-HSQC