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Found 1 Document (1.366 seconds)

BMRB: 5932

1Q75

YNMG tetraloop formation by a dyskeratosis congenita mutation in human telomerase RNA

Authors

Theimer, C.A., Finger, L.D., Feigon, J.

Assembly

DKC mutant P2b telomerase RNA

Entity

1. DKC mutant P2b telomerase RNA (polymer, Thiol state: not present), 15 monomers, 4846.861 Da Detail

GGCUCUCAGU GAGCC

Formula weight

4846.861 Da

Source organism

Homo sapiens

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 97.5 %, Completeness (bb): 100.0 % Detail

Polymer type: polyribonucleotide

	Total	¹H	¹³C	¹⁵N
All	97.5 % (235 of 241)	97.7 % (130 of 133)	100.0 % (100 of 100)	62.5 % (5 of 8)
Suger, PO4	100.0 % (165 of 165)	100.0 % (90 of 90)	100.0 % (75 of 75)
Nucleobase	92.1 % (70 of 76)	93.0 % (40 of 43)	100.0 % (25 of 25)	62.5 % (5 of 8)
Aromatic	90.9 % (60 of 66)	90.9 % (30 of 33)	100.0 % (25 of 25)	62.5 % (5 of 8)

1. human telomerase RNA P2b loop

GGCUCUCAGU GAGCC

Show sample condition

Sample

Pressure 1 atm, Temperature 283 K, pH 6.3

#	Name	Concentration
1	human telomerase RNA P2b loop	1 mM
2	sodium phosphate buffer	10 mM
3	KCl	200 mM
4	EDTA	50 uM
5	sodium azide	0.2 %
6	H2O	95 %
7	D2O	5 %

Release date

2003-12-18

Citation

YNMG tetraloop formation by a dyskeratosis congenita mutation in human telomerase RNA
Theimer, C.A., Finger, L.D., Feigon, J.
RNA (2003), 9, 1446-1455, PubMed 14624001 , Abstract

Related entities 1. DKC mutant P2b telomerase RNA, : 1 entities Detail

Experiments performed 12 experiments Detail

1 2D NOESY (1,1echo and watergate)

Instrument

#1 Model Bruker DRX (600 MHz)

#2 Model Bruker DRX (500 MHz)

Sample #1

Pressure 1 atm, Temperature 283 K, pH 6.3

#	Name	Concentration
1	human telomerase RNA P2b loop	1 mM
2	sodium phosphate buffer	10 mM
3	KCl	200 mM
4	EDTA	50 uM
5	sodium azide	0.2 %
6	H2O	95 %
7	D2O	5 %

Sample #2

Pressure 1 atm, Temperature 283 K, pH 6.3

#	Name	Isotope labeling	Concentration
8	human telomerase RNA P2b loop	[U-13C; U-15N]	1 mM
9	sodium phosphate buffer		10 mM
10	KCl		200 mM
11	EDTA		50 uM
12	sodium azide		0.2 %
13	H2O		95 %
14	D2O		5 %

Sample #3

Pressure 1 atm, Temperature 283 K, pH 6.3

#	Name	Concentration
15	human telomerase RNA P2b loop	1 mM
16	sodium phosphate buffer	10 mM
17	KCl	200 mM
18	EDTA	50 uM
19	sodium azide	0.2 %
20	D2O	100 %

Sample #4

Pressure 1 atm, Temperature 283 K, pH 6.3

#	Name	Isotope labeling	Concentration
21	human telomerase RNA P2b loop	[U-13C; U-15N]	1 mM
22	sodium phosphate buffer		10 mM
23	KCl		200 mM
24	EDTA		50 uM
25	sodium azide		0.2 %
26	D2O		100 %

2 2D 15N-HMQC

Instrument

#1 Model Bruker DRX (600 MHz)

#2 Model Bruker DRX (500 MHz)

Sample #1

Pressure 1 atm, Temperature 283 K, pH 6.3

#	Name	Concentration
1	human telomerase RNA P2b loop	1 mM
2	sodium phosphate buffer	10 mM
3	KCl	200 mM
4	EDTA	50 uM
5	sodium azide	0.2 %
6	H2O	95 %
7	D2O	5 %

Sample #2

Pressure 1 atm, Temperature 283 K, pH 6.3

#	Name	Isotope labeling	Concentration
8	human telomerase RNA P2b loop	[U-13C; U-15N]	1 mM
9	sodium phosphate buffer		10 mM
10	KCl		200 mM
11	EDTA		50 uM
12	sodium azide		0.2 %
13	H2O		95 %
14	D2O		5 %

Sample #3

Pressure 1 atm, Temperature 283 K, pH 6.3

#	Name	Concentration
15	human telomerase RNA P2b loop	1 mM
16	sodium phosphate buffer	10 mM
17	KCl	200 mM
18	EDTA	50 uM
19	sodium azide	0.2 %
20	D2O	100 %

Sample #4

Pressure 1 atm, Temperature 283 K, pH 6.3

#	Name	Isotope labeling	Concentration
21	human telomerase RNA P2b loop	[U-13C; U-15N]	1 mM
22	sodium phosphate buffer		10 mM
23	KCl		200 mM
24	EDTA		50 uM
25	sodium azide		0.2 %
26	D2O		100 %

3 2D 15N-CPMG-NOESY

Instrument

#1 Model Bruker DRX (600 MHz)

#2 Model Bruker DRX (500 MHz)

Sample #1

Pressure 1 atm, Temperature 283 K, pH 6.3

#	Name	Concentration
1	human telomerase RNA P2b loop	1 mM
2	sodium phosphate buffer	10 mM
3	KCl	200 mM
4	EDTA	50 uM
5	sodium azide	0.2 %
6	H2O	95 %
7	D2O	5 %

Sample #2

Pressure 1 atm, Temperature 283 K, pH 6.3

#	Name	Isotope labeling	Concentration
8	human telomerase RNA P2b loop	[U-13C; U-15N]	1 mM
9	sodium phosphate buffer		10 mM
10	KCl		200 mM
11	EDTA		50 uM
12	sodium azide		0.2 %
13	H2O		95 %
14	D2O		5 %

Sample #3

Pressure 1 atm, Temperature 283 K, pH 6.3

#	Name	Concentration
15	human telomerase RNA P2b loop	1 mM
16	sodium phosphate buffer	10 mM
17	KCl	200 mM
18	EDTA	50 uM
19	sodium azide	0.2 %
20	D2O	100 %

Sample #4

Pressure 1 atm, Temperature 283 K, pH 6.3

#	Name	Isotope labeling	Concentration
21	human telomerase RNA P2b loop	[U-13C; U-15N]	1 mM
22	sodium phosphate buffer		10 mM
23	KCl		200 mM
24	EDTA		50 uM
25	sodium azide		0.2 %
26	D2O		100 %

4 2D JNN-HNN-COSY

Instrument

#1 Model Bruker DRX (600 MHz)

#2 Model Bruker DRX (500 MHz)

Sample #1

Pressure 1 atm, Temperature 283 K, pH 6.3

#	Name	Concentration
1	human telomerase RNA P2b loop	1 mM
2	sodium phosphate buffer	10 mM
3	KCl	200 mM
4	EDTA	50 uM
5	sodium azide	0.2 %
6	H2O	95 %
7	D2O	5 %

Sample #2

Pressure 1 atm, Temperature 283 K, pH 6.3

#	Name	Isotope labeling	Concentration
8	human telomerase RNA P2b loop	[U-13C; U-15N]	1 mM
9	sodium phosphate buffer		10 mM
10	KCl		200 mM
11	EDTA		50 uM
12	sodium azide		0.2 %
13	H2O		95 %
14	D2O		5 %

Sample #3

Pressure 1 atm, Temperature 283 K, pH 6.3

#	Name	Concentration
15	human telomerase RNA P2b loop	1 mM
16	sodium phosphate buffer	10 mM
17	KCl	200 mM
18	EDTA	50 uM
19	sodium azide	0.2 %
20	D2O	100 %

Sample #4

Pressure 1 atm, Temperature 283 K, pH 6.3

#	Name	Isotope labeling	Concentration
21	human telomerase RNA P2b loop	[U-13C; U-15N]	1 mM
22	sodium phosphate buffer		10 mM
23	KCl		200 mM
24	EDTA		50 uM
25	sodium azide		0.2 %
26	D2O		100 %

5 2D NOESY

Instrument

#1 Model Bruker DRX (600 MHz)

#2 Model Bruker DRX (500 MHz)

Sample #1

Pressure 1 atm, Temperature 283 K, pH 6.3

#	Name	Concentration
1	human telomerase RNA P2b loop	1 mM
2	sodium phosphate buffer	10 mM
3	KCl	200 mM
4	EDTA	50 uM
5	sodium azide	0.2 %
6	H2O	95 %
7	D2O	5 %

Sample #2

Pressure 1 atm, Temperature 283 K, pH 6.3

#	Name	Isotope labeling	Concentration
8	human telomerase RNA P2b loop	[U-13C; U-15N]	1 mM
9	sodium phosphate buffer		10 mM
10	KCl		200 mM
11	EDTA		50 uM
12	sodium azide		0.2 %
13	H2O		95 %
14	D2O		5 %

Sample #3

Pressure 1 atm, Temperature 283 K, pH 6.3

#	Name	Concentration
15	human telomerase RNA P2b loop	1 mM
16	sodium phosphate buffer	10 mM
17	KCl	200 mM
18	EDTA	50 uM
19	sodium azide	0.2 %
20	D2O	100 %

Sample #4

Pressure 1 atm, Temperature 283 K, pH 6.3

#	Name	Isotope labeling	Concentration
21	human telomerase RNA P2b loop	[U-13C; U-15N]	1 mM
22	sodium phosphate buffer		10 mM
23	KCl		200 mM
24	EDTA		50 uM
25	sodium azide		0.2 %
26	D2O		100 %

6 2D TOCSY

Instrument

#1 Model Bruker DRX (600 MHz)

#2 Model Bruker DRX (500 MHz)

Sample #1

Pressure 1 atm, Temperature 283 K, pH 6.3

#	Name	Concentration
1	human telomerase RNA P2b loop	1 mM
2	sodium phosphate buffer	10 mM
3	KCl	200 mM
4	EDTA	50 uM
5	sodium azide	0.2 %
6	H2O	95 %
7	D2O	5 %

Sample #2

Pressure 1 atm, Temperature 283 K, pH 6.3

#	Name	Isotope labeling	Concentration
8	human telomerase RNA P2b loop	[U-13C; U-15N]	1 mM
9	sodium phosphate buffer		10 mM
10	KCl		200 mM
11	EDTA		50 uM
12	sodium azide		0.2 %
13	H2O		95 %
14	D2O		5 %

Sample #3

Pressure 1 atm, Temperature 283 K, pH 6.3

#	Name	Concentration
15	human telomerase RNA P2b loop	1 mM
16	sodium phosphate buffer	10 mM
17	KCl	200 mM
18	EDTA	50 uM
19	sodium azide	0.2 %
20	D2O	100 %

Sample #4

Pressure 1 atm, Temperature 283 K, pH 6.3

#	Name	Isotope labeling	Concentration
21	human telomerase RNA P2b loop	[U-13C; U-15N]	1 mM
22	sodium phosphate buffer		10 mM
23	KCl		200 mM
24	EDTA		50 uM
25	sodium azide		0.2 %
26	D2O		100 %

7 31P-spin echo difference CT HSQC

Instrument

#1 Model Bruker DRX (600 MHz)

#2 Model Bruker DRX (500 MHz)

Sample #1

Pressure 1 atm, Temperature 283 K, pH 6.3

#	Name	Concentration
1	human telomerase RNA P2b loop	1 mM
2	sodium phosphate buffer	10 mM
3	KCl	200 mM
4	EDTA	50 uM
5	sodium azide	0.2 %
6	H2O	95 %
7	D2O	5 %

Sample #2

Pressure 1 atm, Temperature 283 K, pH 6.3

#	Name	Isotope labeling	Concentration
8	human telomerase RNA P2b loop	[U-13C; U-15N]	1 mM
9	sodium phosphate buffer		10 mM
10	KCl		200 mM
11	EDTA		50 uM
12	sodium azide		0.2 %
13	H2O		95 %
14	D2O		5 %

Sample #3

Pressure 1 atm, Temperature 283 K, pH 6.3

#	Name	Concentration
15	human telomerase RNA P2b loop	1 mM
16	sodium phosphate buffer	10 mM
17	KCl	200 mM
18	EDTA	50 uM
19	sodium azide	0.2 %
20	D2O	100 %

Sample #4

Pressure 1 atm, Temperature 283 K, pH 6.3

#	Name	Isotope labeling	Concentration
21	human telomerase RNA P2b loop	[U-13C; U-15N]	1 mM
22	sodium phosphate buffer		10 mM
23	KCl		200 mM
24	EDTA		50 uM
25	sodium azide		0.2 %
26	D2O		100 %

8 3D 13C-NOESY HMQC

Instrument

#1 Model Bruker DRX (600 MHz)

#2 Model Bruker DRX (500 MHz)

Sample #1

Pressure 1 atm, Temperature 283 K, pH 6.3

#	Name	Concentration
1	human telomerase RNA P2b loop	1 mM
2	sodium phosphate buffer	10 mM
3	KCl	200 mM
4	EDTA	50 uM
5	sodium azide	0.2 %
6	H2O	95 %
7	D2O	5 %

Sample #2

Pressure 1 atm, Temperature 283 K, pH 6.3

#	Name	Isotope labeling	Concentration
8	human telomerase RNA P2b loop	[U-13C; U-15N]	1 mM
9	sodium phosphate buffer		10 mM
10	KCl		200 mM
11	EDTA		50 uM
12	sodium azide		0.2 %
13	H2O		95 %
14	D2O		5 %

Sample #3

Pressure 1 atm, Temperature 283 K, pH 6.3

#	Name	Concentration
15	human telomerase RNA P2b loop	1 mM
16	sodium phosphate buffer	10 mM
17	KCl	200 mM
18	EDTA	50 uM
19	sodium azide	0.2 %
20	D2O	100 %

Sample #4

Pressure 1 atm, Temperature 283 K, pH 6.3

#	Name	Isotope labeling	Concentration
21	human telomerase RNA P2b loop	[U-13C; U-15N]	1 mM
22	sodium phosphate buffer		10 mM
23	KCl		200 mM
24	EDTA		50 uM
25	sodium azide		0.2 %
26	D2O		100 %

9 2D 13C-HMQC

Instrument

#1 Model Bruker DRX (600 MHz)

#2 Model Bruker DRX (500 MHz)

Sample #1

Pressure 1 atm, Temperature 283 K, pH 6.3

#	Name	Concentration
1	human telomerase RNA P2b loop	1 mM
2	sodium phosphate buffer	10 mM
3	KCl	200 mM
4	EDTA	50 uM
5	sodium azide	0.2 %
6	H2O	95 %
7	D2O	5 %

Sample #2

Pressure 1 atm, Temperature 283 K, pH 6.3

#	Name	Isotope labeling	Concentration
8	human telomerase RNA P2b loop	[U-13C; U-15N]	1 mM
9	sodium phosphate buffer		10 mM
10	KCl		200 mM
11	EDTA		50 uM
12	sodium azide		0.2 %
13	H2O		95 %
14	D2O		5 %

Sample #3

Pressure 1 atm, Temperature 283 K, pH 6.3

#	Name	Concentration
15	human telomerase RNA P2b loop	1 mM
16	sodium phosphate buffer	10 mM
17	KCl	200 mM
18	EDTA	50 uM
19	sodium azide	0.2 %
20	D2O	100 %

Sample #4

Pressure 1 atm, Temperature 283 K, pH 6.3

#	Name	Isotope labeling	Concentration
21	human telomerase RNA P2b loop	[U-13C; U-15N]	1 mM
22	sodium phosphate buffer		10 mM
23	KCl		200 mM
24	EDTA		50 uM
25	sodium azide		0.2 %
26	D2O		100 %

10 2D HCCH COSY

Instrument

#1 Model Bruker DRX (600 MHz)

#2 Model Bruker DRX (500 MHz)

Sample #1

Pressure 1 atm, Temperature 283 K, pH 6.3

#	Name	Concentration
1	human telomerase RNA P2b loop	1 mM
2	sodium phosphate buffer	10 mM
3	KCl	200 mM
4	EDTA	50 uM
5	sodium azide	0.2 %
6	H2O	95 %
7	D2O	5 %

Sample #2

Pressure 1 atm, Temperature 283 K, pH 6.3

#	Name	Isotope labeling	Concentration
8	human telomerase RNA P2b loop	[U-13C; U-15N]	1 mM
9	sodium phosphate buffer		10 mM
10	KCl		200 mM
11	EDTA		50 uM
12	sodium azide		0.2 %
13	H2O		95 %
14	D2O		5 %

Sample #3

Pressure 1 atm, Temperature 283 K, pH 6.3

#	Name	Concentration
15	human telomerase RNA P2b loop	1 mM
16	sodium phosphate buffer	10 mM
17	KCl	200 mM
18	EDTA	50 uM
19	sodium azide	0.2 %
20	D2O	100 %

Sample #4

Pressure 1 atm, Temperature 283 K, pH 6.3

#	Name	Isotope labeling	Concentration
21	human telomerase RNA P2b loop	[U-13C; U-15N]	1 mM
22	sodium phosphate buffer		10 mM
23	KCl		200 mM
24	EDTA		50 uM
25	sodium azide		0.2 %
26	D2O		100 %

11 3D HCCH TOCSY

Instrument

#1 Model Bruker DRX (600 MHz)

#2 Model Bruker DRX (500 MHz)

Sample #1

Pressure 1 atm, Temperature 283 K, pH 6.3

#	Name	Concentration
1	human telomerase RNA P2b loop	1 mM
2	sodium phosphate buffer	10 mM
3	KCl	200 mM
4	EDTA	50 uM
5	sodium azide	0.2 %
6	H2O	95 %
7	D2O	5 %

Sample #2

Pressure 1 atm, Temperature 283 K, pH 6.3

#	Name	Isotope labeling	Concentration
8	human telomerase RNA P2b loop	[U-13C; U-15N]	1 mM
9	sodium phosphate buffer		10 mM
10	KCl		200 mM
11	EDTA		50 uM
12	sodium azide		0.2 %
13	H2O		95 %
14	D2O		5 %

Sample #3

Pressure 1 atm, Temperature 283 K, pH 6.3

#	Name	Concentration
15	human telomerase RNA P2b loop	1 mM
16	sodium phosphate buffer	10 mM
17	KCl	200 mM
18	EDTA	50 uM
19	sodium azide	0.2 %
20	D2O	100 %

Sample #4

Pressure 1 atm, Temperature 283 K, pH 6.3

#	Name	Isotope labeling	Concentration
21	human telomerase RNA P2b loop	[U-13C; U-15N]	1 mM
22	sodium phosphate buffer		10 mM
23	KCl		200 mM
24	EDTA		50 uM
25	sodium azide		0.2 %
26	D2O		100 %

12 CT-CE-HSQC

Instrument

#1 Model Bruker DRX (600 MHz)

#2 Model Bruker DRX (500 MHz)

Sample #1

Pressure 1 atm, Temperature 283 K, pH 6.3

#	Name	Concentration
1	human telomerase RNA P2b loop	1 mM
2	sodium phosphate buffer	10 mM
3	KCl	200 mM
4	EDTA	50 uM
5	sodium azide	0.2 %
6	H2O	95 %
7	D2O	5 %

Sample #2

Pressure 1 atm, Temperature 283 K, pH 6.3

#	Name	Isotope labeling	Concentration
8	human telomerase RNA P2b loop	[U-13C; U-15N]	1 mM
9	sodium phosphate buffer		10 mM
10	KCl		200 mM
11	EDTA		50 uM
12	sodium azide		0.2 %
13	H2O		95 %
14	D2O		5 %

Sample #3

Pressure 1 atm, Temperature 283 K, pH 6.3

#	Name	Concentration
15	human telomerase RNA P2b loop	1 mM
16	sodium phosphate buffer	10 mM
17	KCl	200 mM
18	EDTA	50 uM
19	sodium azide	0.2 %
20	D2O	100 %

Sample #4

Pressure 1 atm, Temperature 283 K, pH 6.3

#	Name	Isotope labeling	Concentration
21	human telomerase RNA P2b loop	[U-13C; U-15N]	1 mM
22	sodium phosphate buffer		10 mM
23	KCl		200 mM
24	EDTA		50 uM
25	sodium azide		0.2 %
26	D2O		100 %

nullKeywords PENTALOOP, TETRALOOP, UUCG, YNMG

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Found 1 Document (1.366 seconds)

BMRB: 5932

Sample

Related entities 1. DKC mutant P2b telomerase RNA, : 1 entities Detail

Experiments performed 12 experiments Detail

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