BMRBj - BMREntryMaster

Found 1 Document (2.116 seconds)

BMRB: 5933

1X9X

Sequence-specific backbone and sidechain resonance assignments of the Ste50 binding domain of the MAPKKK Ste11

Authors

Bhattacharjya, S., Xu, P., Shaykhutdinov, R., Gingras, R., Ni, F.

Assembly

Ste11 Regulatory Domain

Entity

1. Ste11 Regulatory Domain (polymer, Thiol state: all free), 68 monomers, 7950.100 × 2 Da Detail

DEKTNDLPFV QLFLEEIGCT QYLDSFIQCN LVTEEEIKYL DKDILIALGV NKIGDRLKIL RKSKSFQR

Total weight

15900.2 Da

Max. entity weight

7950.1 Da

Source organism

Saccharomyces cerevisiae

Exptl. method

NMR

Refine. method

distant geometry

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 92.4 %, Completeness (bb): 96.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	92.4 % (782 of 846)	91.9 % (408 of 444)	92.4 % (303 of 328)	95.9 % (71 of 74)
Backbone	96.8 % (393 of 406)	96.4 % (133 of 138)	97.0 % (195 of 201)	97.0 % (65 of 67)
Sidechain	89.7 % (453 of 505)	89.9 % (275 of 306)	89.6 % (172 of 192)	85.7 % (6 of 7)
Aromatic	42.9 % (24 of 56)	42.9 % (12 of 28)	42.9 % (12 of 28)
Methyl	95.5 % (84 of 88)	95.5 % (42 of 44)	95.5 % (42 of 44)

1. SAM domain of Ste11

DEKTNDLPFV QLFLEEIGCT QYLDSFIQCN LVTEEEIKYL DKDILIALGV NKIGDRLKIL RKSKSFQR

Show sample condition

Sample #1

Temperature 288 (±1.0) K, pH 5.8 (±0.1)

#	Name	Isotope labeling	Concentration
1	SAM domain of Ste11	[U-15N]	0.8 mM
2	K-phosphate buffer		10 mM
3	NaCl		300 mM

Sample #2

Temperature 288 (±1.0) K, pH 5.8 (±0.1)

#	Name	Isotope labeling	Concentration
4	SAM domain of Ste11	[U-15N; U-13C]	0.8 mM
5	K-phosphate buffer		10 mM
6	NaCl		300 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.101329
¹³C	DSS	methyl protons	null	indirect	0.251449

Referencing offset: -0.16 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.101329
¹³C	DSS	methyl protons	null	indirect	0.251449

Referencing offset: -0.16 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.101329
¹³C	DSS	methyl protons	null	indirect	0.251449

Referencing offset: -0.04 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.101329
¹³C	DSS	methyl protons	null	indirect	0.251449

Referencing offset: -0.54 ppm, Outliers: 3 Detail

Protein Blocks Logo

Calculated from 8 models in PDB: 1X9X, Strand ID: A, B Detail

Release date

2005-02-28

Citation

Solution structure of the dimeric SAM domain of MAPKKK Ste11 and its interactions with the adaptor protein Ste50 from the budding yeast: implications for Ste11 activation and signal transmission through the Ste50-Ste11 complex
Bhattacharjya, S., Xu, P., Gingras, R., Shaykhutdinov, R., Wu, C., Whiteway, M., Ni, F.
J. Mol. Biol. (2004), 344, 1071-1087, PubMed 15544813 , DOI 10.1016/j.jmb.2004.09.018 , Abstract

Related entities 1. Ste11 Regulatory Domain, : 1 : 5 : 49 entities Detail

Interaction partners 1. Ste11 Regulatory Domain, : 31 interactors Detail

More details for 31 interactors identified by 15 citations

Experiments performed 11 experiments Detail

1 2D (1H-15N) HSQC

Instrument

Model Bruker DRX (800 MHz)

Sample #1

Temperature 288 (±1.0) K, pH 5.8 (±0.1)

#	Name	Isotope labeling	Concentration
1	SAM domain of Ste11	[U-15N]	0.8 mM
2	K-phosphate buffer		10 mM
3	NaCl		300 mM

Sample #2

Temperature 288 (±1.0) K, pH 5.8 (±0.1)

#	Name	Isotope labeling	Concentration
4	SAM domain of Ste11	[U-15N; U-13C]	0.8 mM
5	K-phosphate buffer		10 mM
6	NaCl		300 mM

2 2D (1H-1H) NOESY

Instrument

Model Bruker DRX (800 MHz)

Sample #1

Temperature 288 (±1.0) K, pH 5.8 (±0.1)

#	Name	Isotope labeling	Concentration
1	SAM domain of Ste11	[U-15N]	0.8 mM
2	K-phosphate buffer		10 mM
3	NaCl		300 mM

Sample #2

Temperature 288 (±1.0) K, pH 5.8 (±0.1)

#	Name	Isotope labeling	Concentration
4	SAM domain of Ste11	[U-15N; U-13C]	0.8 mM
5	K-phosphate buffer		10 mM
6	NaCl		300 mM

3 3D (1H-15N) NOESY-HSQC

Instrument

Model Bruker DRX (800 MHz)

Sample #1

Temperature 288 (±1.0) K, pH 5.8 (±0.1)

#	Name	Isotope labeling	Concentration
1	SAM domain of Ste11	[U-15N]	0.8 mM
2	K-phosphate buffer		10 mM
3	NaCl		300 mM

Sample #2

Temperature 288 (±1.0) K, pH 5.8 (±0.1)

#	Name	Isotope labeling	Concentration
4	SAM domain of Ste11	[U-15N; U-13C]	0.8 mM
5	K-phosphate buffer		10 mM
6	NaCl		300 mM

4 3D (1H-15N) TOCSY-HSQC

Instrument

Model Bruker DRX (800 MHz)

Sample #1

Temperature 288 (±1.0) K, pH 5.8 (±0.1)

#	Name	Isotope labeling	Concentration
1	SAM domain of Ste11	[U-15N]	0.8 mM
2	K-phosphate buffer		10 mM
3	NaCl		300 mM

Sample #2

Temperature 288 (±1.0) K, pH 5.8 (±0.1)

#	Name	Isotope labeling	Concentration
4	SAM domain of Ste11	[U-15N; U-13C]	0.8 mM
5	K-phosphate buffer		10 mM
6	NaCl		300 mM

5 3D HNCA

Instrument

Model Bruker DRX (800 MHz)

Sample #1

Temperature 288 (±1.0) K, pH 5.8 (±0.1)

#	Name	Isotope labeling	Concentration
1	SAM domain of Ste11	[U-15N]	0.8 mM
2	K-phosphate buffer		10 mM
3	NaCl		300 mM

Sample #2

Temperature 288 (±1.0) K, pH 5.8 (±0.1)

#	Name	Isotope labeling	Concentration
4	SAM domain of Ste11	[U-15N; U-13C]	0.8 mM
5	K-phosphate buffer		10 mM
6	NaCl		300 mM

6 3D HN(CO)CA

Instrument

Model Bruker DRX (800 MHz)

Sample #1

Temperature 288 (±1.0) K, pH 5.8 (±0.1)

#	Name	Isotope labeling	Concentration
1	SAM domain of Ste11	[U-15N]	0.8 mM
2	K-phosphate buffer		10 mM
3	NaCl		300 mM

Sample #2

Temperature 288 (±1.0) K, pH 5.8 (±0.1)

#	Name	Isotope labeling	Concentration
4	SAM domain of Ste11	[U-15N; U-13C]	0.8 mM
5	K-phosphate buffer		10 mM
6	NaCl		300 mM

7 3D CBCA(CO)NH

Instrument

Model Bruker DRX (800 MHz)

Sample #1

Temperature 288 (±1.0) K, pH 5.8 (±0.1)

#	Name	Isotope labeling	Concentration
1	SAM domain of Ste11	[U-15N]	0.8 mM
2	K-phosphate buffer		10 mM
3	NaCl		300 mM

Sample #2

Temperature 288 (±1.0) K, pH 5.8 (±0.1)

#	Name	Isotope labeling	Concentration
4	SAM domain of Ste11	[U-15N; U-13C]	0.8 mM
5	K-phosphate buffer		10 mM
6	NaCl		300 mM

8 3D HNCO

Instrument

Model Bruker DRX (800 MHz)

Sample #1

Temperature 288 (±1.0) K, pH 5.8 (±0.1)

#	Name	Isotope labeling	Concentration
1	SAM domain of Ste11	[U-15N]	0.8 mM
2	K-phosphate buffer		10 mM
3	NaCl		300 mM

Sample #2

Temperature 288 (±1.0) K, pH 5.8 (±0.1)

#	Name	Isotope labeling	Concentration
4	SAM domain of Ste11	[U-15N; U-13C]	0.8 mM
5	K-phosphate buffer		10 mM
6	NaCl		300 mM

9 3D HBHA(C0)NH

Instrument

Model Bruker DRX (800 MHz)

Sample #1

Temperature 288 (±1.0) K, pH 5.8 (±0.1)

#	Name	Isotope labeling	Concentration
1	SAM domain of Ste11	[U-15N]	0.8 mM
2	K-phosphate buffer		10 mM
3	NaCl		300 mM

Sample #2

Temperature 288 (±1.0) K, pH 5.8 (±0.1)

#	Name	Isotope labeling	Concentration
4	SAM domain of Ste11	[U-15N; U-13C]	0.8 mM
5	K-phosphate buffer		10 mM
6	NaCl		300 mM

10 3D (H)CCH-COSY

Instrument

Model Bruker DRX (800 MHz)

Sample #1

Temperature 288 (±1.0) K, pH 5.8 (±0.1)

#	Name	Isotope labeling	Concentration
1	SAM domain of Ste11	[U-15N]	0.8 mM
2	K-phosphate buffer		10 mM
3	NaCl		300 mM

Sample #2

Temperature 288 (±1.0) K, pH 5.8 (±0.1)

#	Name	Isotope labeling	Concentration
4	SAM domain of Ste11	[U-15N; U-13C]	0.8 mM
5	K-phosphate buffer		10 mM
6	NaCl		300 mM

11 3D (1H-13C) NOESY-HSQC

Instrument

Model Bruker DRX (800 MHz)

Sample #1

Temperature 288 (±1.0) K, pH 5.8 (±0.1)

#	Name	Isotope labeling	Concentration
1	SAM domain of Ste11	[U-15N]	0.8 mM
2	K-phosphate buffer		10 mM
3	NaCl		300 mM

Sample #2

Temperature 288 (±1.0) K, pH 5.8 (±0.1)

#	Name	Isotope labeling	Concentration
4	SAM domain of Ste11	[U-15N; U-13C]	0.8 mM
5	K-phosphate buffer		10 mM
6	NaCl		300 mM

nullKeywords Ste11, SAM domain, Ste50, heteronuclear NMR

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Found 1 Document (2.116 seconds)

BMRB: 5933

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Related entities 1. Ste11 Regulatory Domain, : 1 : 5 : 49 entities Detail

Interaction partners 1. Ste11 Regulatory Domain, : 31 interactors Detail

Experiments performed 11 experiments Detail

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