BMRB: 5935

Sequence-specific 1H, 13C and 15N resonance assignments of rat liver fructose-2,6-bisphosphatase domain

Authors

Zangger, K., Pervushin, K., Sterk, H., Lange, A.J., Okar, D.A.

Assembly

fructose-2,6-bisphosphatase

Entity

1. fructose-2,6-bisphosphatase (polymer, Thiol state: all free), 221 monomers, 25553.69 Da Detail

MRSIYLCRHG ESELNLRGRI GGDSGLSARG KQYAYALANF IRSQGISSLK VWTSHMKRTI QTAEALGVPY EQWKALNEID AGVCEEMTYE EIQEHYPEEF ALRDQDKYRY RYPKGESYED LVQRLEPVIM ELERQENVLV ICHQAVMRCL LAYFLDKSSD ELPYLKCPLH TVLKLTPVAY GCRVESIYLN VEAVNTHRDK PENVDITREA EEALDTVPAH Y

Formula weight

25553.69 Da

Source organism

Rattus norvegicus

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 91.0 %, Completeness: 39.3 %, Completeness (bb): 69.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	39.3 % (1023 of 2603)	19.5 % (265 of 1357)	58.1 % (590 of 1016)	73.0 % (168 of 230)
Backbone	69.0 % (903 of 1308)	42.8 % (190 of 444)	83.7 % (546 of 652)	78.8 % (167 of 212)
Sidechain	18.8 % (283 of 1505)	8.2 % (75 of 913)	36.1 % (207 of 574)	5.6 % (1 of 18)
Aromatic	3.0 % (6 of 202)	4.0 % (4 of 101)	2.0 % (2 of 99)	0.0 % (0 of 2)
Methyl	17.5 % (43 of 246)	8.1 % (10 of 123)	26.8 % (33 of 123)

1. Fructose-2,6-bisphosphatase

MRSIYLCRHG ESELNLRGRI GGDSGLSARG KQYAYALANF IRSQGISSLK VWTSHMKRTI QTAEALGVPY EQWKALNEID AGVCEEMTYE EIQEHYPEEF ALRDQDKYRY RYPKGESYED LVQRLEPVIM ELERQENVLV ICHQAVMRCL LAYFLDKSSD ELPYLKCPLH TVLKLTPVAY GCRVESIYLN VEAVNTHRDK PENVDITREA EEALDTVPAH Y

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Sample

Temperature 298 (±1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Fructose-2,6-bisphosphatase	[U-95% 13C; U-95 15N]		2.7 mM
2	sodium phosphate			100 mM

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Calculated from 1 models in PDB: 1TIP, Strand ID: A, B Detail

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Release date

2003-10-05

Citation 1

Sequence-specific 1H, 13C and 15N resonance assignments of the rat liver fructose-2,6-bisphosphatase domain
Zangger, K., Pervushin, K., Sterk, H., Lange, A.J., Okar, D.A.
J. Biomol. NMR (2003), 27, 281-282, PubMed 12975587 , DOI: , Abstract

Show more 3 citaions

Citation 2

NMRPipe: a multidimensional spectral processing system based on UNIX pipes
Delaglio, F., Grzesiek, S., Vuister, G.W., Zhu, G., Pfeifer, J., Bax, A.
J. Biomol. NMR (1995), 6, 277-293, PubMed 8520220 , Abstract

Citation 3

Crystal structure of the rat liver fructose-2,6-bisphosphatase based on selenomethionine multiwavelength anomalous dispersion phases
Lee, Y.H., Ogata, C., Pflugrath, J.W., Levitt, D.G., Sarma, R., Banaszak, L.J., Pilkis, S.J.
Biochemistry (1996), 35, 6010-6019, PubMed 8634242 , DOI 10.1021/bi9600613 , Abstract

Citation 4

The crystal structure of the bifunctional enzyme 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase reveals distinct domain homologies
Hasemann, C.A., Istvan, E.S., Uyeda, K., Deisenhofer, J.
Structure (1996), 4, 1017-1029, PubMed 8805587 , DOI: , Abstract

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Related entities 1. fructose-2,6-bisphosphatase, : 1 : 8 : 108 entities Detail

Interaction partners 1. fructose-2,6-bisphosphatase, : 2 interactors Detail

Experiments performed 2 experiments Detail