BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	82.0 % (1190 of 1452)	91.3 % (691 of 757)	65.7 % (372 of 566)	98.4 % (127 of 129)
Backbone	83.8 % (625 of 746)	97.3 % (253 of 260)	69.0 % (252 of 365)	99.2 % (120 of 121)
Sidechain	82.7 % (677 of 819)	88.1 % (438 of 497)	73.9 % (232 of 314)	87.5 % (7 of 8)
Aromatic	62.5 % (80 of 128)	90.6 % (58 of 64)	33.9 % (21 of 62)	50.0 % (1 of 2)
Methyl	77.5 % (93 of 120)	93.3 % (56 of 60)	61.7 % (37 of 60)

1. murine Spred2 EVH1 domain

GSMTEETHPD DDSYIVRVKA VVMTRDDSSG GWFPQEGGGI SRVGVCKVMH PEGNGRSGFL IHGERQKDKL VVLECYVRKD LVYTKANPTF HHWKVDNRKF GLTFQSPADA RAFDRGVRKA IEDLIE

Show sample condition

Sample #1

Temperature 300 (±2) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	murine Spred2 EVH1 domain	[U-15N]		1.0 mM
2	H2O			100 %

Sample #2

Temperature 300 (±2) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
3	murine Spred2 EVH1 domain	[U-15N; U-13C]		1.0 mM
4	H2O			100 %

Protein Blocks Logo

Calculated from 20 models in PDB: 2JP2, Strand ID: A Detail

Release date

2005-02-28

Citation

1H, 13C and 15N resonance assignment of the human Spred2 EVH1 domain
Zimmermann, J., Jarchau, T., Walter, U., Oschkinat, H., Ball, L.J.
J. Biomol. NMR (2004), 29, 435-436, PubMed 15213456 , DOI 10.1023/B:JNMR.0000032526.17586.8c , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Swiss-Prot: Q7Z698 released on 2005-01-04
Title SPRE2_HUMAN Entity Sprouty-related, EVH1 domain-containing protein 2

Related entities 1. Spred2 EVH1 domain, : 1 : 7 : 76 entities Detail

Interaction partners 1. Spred2 EVH1 domain, : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Experiments performed 12 experiments Detail

1 15N-NOESY-HSQC

Instrument

#1 Model Bruker DMX (750 MHz)

#2 Model Bruker DRX (600 MHz)

Sample #1

Temperature 300 (±2) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	murine Spred2 EVH1 domain	[U-15N]		1.0 mM
2	H2O			100 %

Sample #2

Temperature 300 (±2) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
3	murine Spred2 EVH1 domain	[U-15N; U-13C]		1.0 mM
4	H2O			100 %

Sample #3

Temperature 300 (±2) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
5	murine Spred2 EVH1 domain	[U-15N; U-13C]		1.0 mM
6	D2O			100 %

2 13C-NOESY-HSQC

Instrument

#1 Model Bruker DMX (750 MHz)

#2 Model Bruker DRX (600 MHz)

Sample #1

Temperature 300 (±2) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	murine Spred2 EVH1 domain	[U-15N]		1.0 mM
2	H2O			100 %

Sample #2

Temperature 300 (±2) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
3	murine Spred2 EVH1 domain	[U-15N; U-13C]		1.0 mM
4	H2O			100 %

Sample #3

Temperature 300 (±2) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
5	murine Spred2 EVH1 domain	[U-15N; U-13C]		1.0 mM
6	D2O			100 %

3 13C-HMQC-NOESY

Instrument

#1 Model Bruker DMX (750 MHz)

#2 Model Bruker DRX (600 MHz)

Sample #1

Temperature 300 (±2) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	murine Spred2 EVH1 domain	[U-15N]		1.0 mM
2	H2O			100 %

Sample #2

Temperature 300 (±2) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
3	murine Spred2 EVH1 domain	[U-15N; U-13C]		1.0 mM
4	H2O			100 %

Sample #3

Temperature 300 (±2) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
5	murine Spred2 EVH1 domain	[U-15N; U-13C]		1.0 mM
6	D2O			100 %

4 CBCACONNH

Instrument

#1 Model Bruker DMX (750 MHz)

#2 Model Bruker DRX (600 MHz)

Sample #1

Temperature 300 (±2) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	murine Spred2 EVH1 domain	[U-15N]		1.0 mM
2	H2O			100 %

Sample #2

Temperature 300 (±2) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
3	murine Spred2 EVH1 domain	[U-15N; U-13C]		1.0 mM
4	H2O			100 %

Sample #3

Temperature 300 (±2) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
5	murine Spred2 EVH1 domain	[U-15N; U-13C]		1.0 mM
6	D2O			100 %

5 CBCANNH

Instrument

#1 Model Bruker DMX (750 MHz)

#2 Model Bruker DRX (600 MHz)

Sample #1

Temperature 300 (±2) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	murine Spred2 EVH1 domain	[U-15N]		1.0 mM
2	H2O			100 %

Sample #2

Temperature 300 (±2) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
3	murine Spred2 EVH1 domain	[U-15N; U-13C]		1.0 mM
4	H2O			100 %

Sample #3

Temperature 300 (±2) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
5	murine Spred2 EVH1 domain	[U-15N; U-13C]		1.0 mM
6	D2O			100 %

6 15N-HSQC

Instrument

#1 Model Bruker DMX (750 MHz)

#2 Model Bruker DRX (600 MHz)

Sample #1

Temperature 300 (±2) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	murine Spred2 EVH1 domain	[U-15N]		1.0 mM
2	H2O			100 %

Sample #2

Temperature 300 (±2) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
3	murine Spred2 EVH1 domain	[U-15N; U-13C]		1.0 mM
4	H2O			100 %

Sample #3

Temperature 300 (±2) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
5	murine Spred2 EVH1 domain	[U-15N; U-13C]		1.0 mM
6	D2O			100 %

7 CCCONNH

Instrument

#1 Model Bruker DMX (750 MHz)

#2 Model Bruker DRX (600 MHz)

Sample #1

Temperature 300 (±2) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	murine Spred2 EVH1 domain	[U-15N]		1.0 mM
2	H2O			100 %

Sample #2

Temperature 300 (±2) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
3	murine Spred2 EVH1 domain	[U-15N; U-13C]		1.0 mM
4	H2O			100 %

Sample #3

Temperature 300 (±2) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
5	murine Spred2 EVH1 domain	[U-15N; U-13C]		1.0 mM
6	D2O			100 %

8 HCCCONNH

Instrument

#1 Model Bruker DMX (750 MHz)

#2 Model Bruker DRX (600 MHz)

Sample #1

Temperature 300 (±2) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	murine Spred2 EVH1 domain	[U-15N]		1.0 mM
2	H2O			100 %

Sample #2

Temperature 300 (±2) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
3	murine Spred2 EVH1 domain	[U-15N; U-13C]		1.0 mM
4	H2O			100 %

Sample #3

Temperature 300 (±2) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
5	murine Spred2 EVH1 domain	[U-15N; U-13C]		1.0 mM
6	D2O			100 %

9 2D-NOESY

Instrument

#1 Model Bruker DMX (750 MHz)

#2 Model Bruker DRX (600 MHz)

Sample #1

Temperature 300 (±2) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	murine Spred2 EVH1 domain	[U-15N]		1.0 mM
2	H2O			100 %

Sample #2

Temperature 300 (±2) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
3	murine Spred2 EVH1 domain	[U-15N; U-13C]		1.0 mM
4	H2O			100 %

Sample #3

Temperature 300 (±2) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
5	murine Spred2 EVH1 domain	[U-15N; U-13C]		1.0 mM
6	D2O			100 %

10 2D-TOCSY

Instrument

#1 Model Bruker DMX (750 MHz)

#2 Model Bruker DRX (600 MHz)

Sample #1

Temperature 300 (±2) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	murine Spred2 EVH1 domain	[U-15N]		1.0 mM
2	H2O			100 %

Sample #2

Temperature 300 (±2) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
3	murine Spred2 EVH1 domain	[U-15N; U-13C]		1.0 mM
4	H2O			100 %

Sample #3

Temperature 300 (±2) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
5	murine Spred2 EVH1 domain	[U-15N; U-13C]		1.0 mM
6	D2O			100 %

11 2D-COSY

Instrument

#1 Model Bruker DMX (750 MHz)

#2 Model Bruker DRX (600 MHz)

Sample #1

Temperature 300 (±2) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	murine Spred2 EVH1 domain	[U-15N]		1.0 mM
2	H2O			100 %

Sample #2

Temperature 300 (±2) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
3	murine Spred2 EVH1 domain	[U-15N; U-13C]		1.0 mM
4	H2O			100 %

Sample #3

Temperature 300 (±2) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
5	murine Spred2 EVH1 domain	[U-15N; U-13C]		1.0 mM
6	D2O			100 %

12 15N-TOCSY

Instrument

#1 Model Bruker DMX (750 MHz)

#2 Model Bruker DRX (600 MHz)

Sample #1

Temperature 300 (±2) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	murine Spred2 EVH1 domain	[U-15N]		1.0 mM
2	H2O			100 %

Sample #2

Temperature 300 (±2) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
3	murine Spred2 EVH1 domain	[U-15N; U-13C]		1.0 mM
4	H2O			100 %

Sample #3

Temperature 300 (±2) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
5	murine Spred2 EVH1 domain	[U-15N; U-13C]		1.0 mM
6	D2O			100 %

nullKeywords NMR structure, EVH1 domains, automated assignment

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BMRB: 5939

Sample #1

Sample #2

Entries sharing articles Swiss-Prot: 1 entries Detail

Related entities 1. Spred2 EVH1 domain, : 1 : 7 : 76 entities Detail

Interaction partners 1. Spred2 EVH1 domain, : 1 interactors Detail

Experiments performed 12 experiments Detail

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