BMRB: 5945

A Zinc Clasp Structure Tethers Lck to T Cell Coreceptors CD4 and CD8

Authors

Kim, P.W., Sun, Z.J., Blacklow, S.C., Wagner, G., Eck, M.J.

Assembly

CD8a cytoplasmic tail - Lck N terminus

Entity

1. CD8a (polymer, Thiol state: free and other bound), 19 monomers, 2254.686 Da Detail

RNRRRVCKCP RPVVKSGDK

2. Lck (polymer, Thiol state: free and other bound), 29 monomers, 3296.552 Da Detail

SHPEDDWLEN IDVCENCHYP IVPLDGKGT

3. ZN (non-polymer), 65.409 Da

Total weight

5616.6475 Da

Max. entity weight

3296.552 Da

Entity Connection

metal coordination 4 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	metal coordination	sing	1:CYS7:SG	3:ZN1:ZN
2	metal coordination	sing	1:CYS9:SG	3:ZN1:ZN
3	metal coordination	sing	2:CYS14:SG	3:ZN1:ZN
4	metal coordination	sing	2:CYS17:SG	3:ZN1:ZN

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

distance geometry, simulated annealing, molecular dynamics, torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete1

Sequence coverage: 45.8 %, Completeness: 34.2 %, Completeness (bb): 32.7 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	34.2 % (190 of 555)	36.1 % (107 of 296)	31.1 % (66 of 212)	36.2 % (17 of 47)
Backbone	32.7 % (91 of 278)	40.4 % (38 of 94)	26.2 % (37 of 141)	37.2 % (16 of 43)
Sidechain	36.3 % (117 of 322)	34.2 % (69 of 202)	40.5 % (47 of 116)	25.0 % (1 of 4)
Aromatic	0.0 % (0 of 28)	0.0 % (0 of 14)	0.0 % (0 of 13)	0.0 % (0 of 1)
Methyl	31.6 % (12 of 38)	31.6 % (6 of 19)	31.6 % (6 of 19)

1. CD8a

RNRRRVCKCP RPVVKSGDK

2. Lck

SHPEDDWLEN IDVCENCHYP IVPLDGKGT

Show sample condition

Sample

Temperature 298 (±1) K, pH 5.1 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	CD8a			0.5 mM
2	Lck			0.5 mM

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Chem. Shift Complete2

Sequence coverage: 58.3 %, Completeness: 39.5 %, Completeness (bb): 39.9 % Detail

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• BMRB/XML (partial)
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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	39.5 % (438 of 1110)	41.0 % (243 of 592)	36.1 % (153 of 424)	44.7 % (42 of 94)
Backbone	39.9 % (222 of 556)	47.9 % (90 of 188)	32.6 % (92 of 282)	46.5 % (40 of 86)
Sidechain	40.4 % (260 of 644)	37.9 % (153 of 404)	45.3 % (105 of 232)	25.0 % (2 of 8)
Aromatic	28.6 % (16 of 56)	39.3 % (11 of 28)	15.4 % (4 of 26)	50.0 % (1 of 2)
Methyl	48.7 % (37 of 76)	50.0 % (19 of 38)	47.4 % (18 of 38)

1. CD8a

RNRRRVCKCP RPVVKSGDK

2. Lck

SHPEDDWLEN IDVCENCHYP IVPLDGKGT

Show sample condition

Sample

Temperature 298 (±1) K, pH 5.1 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	CD8a			0.5 mM
2	Lck			0.5 mM

Hide sample codition

Protein Blocks Logo

Calculated from 10 models in PDB: 1Q69, Strand ID: A, B Detail

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Release date

2003-12-18

Citation

A zinc clasp structure tethers Lck to T cell coreceptors CD4 and CD8
Kim, P.W., Sun, Z.J., Blacklow, S.C., Wagner, G., Eck, M.J.
Science (2003), 301, 1725-1728, PubMed 14500983 , DOI 10.1126/science.1085643 , Abstract

Entries sharing articles BMRB: 1, Swiss-Prot: 1 entries Detail

Related entities 1. CD8a, : 1 : 2 : 1 : 6 entities Detail

Related entities 2. Lck, : 1 : 1 : 2 : 20 entities Detail

Interaction partners 1. CD8a, : 9 interactors Detail

Interaction partners 2. Lck, : 62 interactors Detail

Experiments performed 13 experiments Detail