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BMRB: 5946


5946
2FVN
Complete Resonance assignments of a donor-strand complemented AfaD: the afimbrial invasin from Diffusely Adherent E. coli
Authors
Cota, E., Anderson, K., Peter, S., Ho Ann, C., Bogdan, N., Josef, K.
Assembly
afaD-dsc
Entity
1. afaD-dsc (polymer, Thiol state: not reported), 153 monomers, 16601.07 Da Detail

MRGSHHHHHH GSAELHLESR GGSGTQLRDG AKVATGRIIC REAHTGFHVW MNERQVDGRA ERYVVQSKDG RHELRVRTGG DGWSPVKGEG GKGVSRPGQE EQVFFDVMAD GNQDIAPGEY RFSVGGACVV PQEDNKQGFT PSGTTGTTKL TVT


Formula weight
16601.07 Da
Source organism
Escherichia coli
Exptl. method
NMR
Refine. method
Aria-implemented distance geometry, simulated annealing
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 92.8 %, Completeness: 83.7 %, Completeness (bb): 89.4 % Detail
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Polymer type: polypeptide(L)

Total1H13C15N
All83.7 % (1396 of 1667)82.9 % (719 of 867)84.8 % (542 of 639)83.9 % (135 of 161)
Backbone89.4 % (812 of 908)90.8 % (296 of 326)88.2 % (383 of 434)89.9 % (133 of 148)
Sidechain79.0 % (701 of 887)78.2 % (423 of 541)82.9 % (276 of 333)15.4 % (2 of 13)
Aromatic66.2 % (86 of 130)66.2 % (43 of 65)65.1 % (41 of 63)100.0 % (2 of 2)
Methyl88.9 % (112 of 126)88.9 % (56 of 63)88.9 % (56 of 63)

1. afaD-III

MRGSHHHHHH GSAELHLESR GGSGTQLRDG AKVATGRIIC REAHTGFHVW MNERQVDGRA ERYVVQSKDG RHELRVRTGG DGWSPVKGEG GKGVSRPGQE EQVFFDVMAD GNQDIAPGEY RFSVGGACVV PQEDNKQGFT PSGTTGTTKL TVT

Show sample condition
Sample

Temperature 303 (±0.2) K, pH 7.0 (±0.1)


#NameIsotope labelingTypeConcentration
1afaD-III0.8 mM

LACS Plot; CA
Referencing offset: -0.54 ppm, Outliers: 1 Detail
LACS Plot; CB
Referencing offset: -0.54 ppm, Outliers: 1 Detail
LACS Plot; HA
Referencing offset: -0.08 ppm, Outliers: 1 Detail
LACS Plot; CO
Referencing offset: 1.17 ppm, Outliers: 1 Detail
Protein Blocks Logo
Calculated from 1 models in PDB: 2FVN, Strand ID: A Detail

Release date
2004-07-05
Citation 1
Complete resonance assignments of the 'donor-strand complemented' AfaD: the afimbrial invasin from Diffusely Adherent E. coli
Cota, E., Chen, H., Anderson, K.L., Simpson, P., du Merle, L., Bernier-Febreau, C., Piatek, R., Zalewska, B., Nowicki, B., Kur, J., Le Bouguenec, C., Matthews, S.
J. Biomol. NMR (2004), 29, 411-412, PubMed 15213444 , DOI 10.1023/B:JNMR.0000032499.61022.45 ,
Show more 1 citaion
Citation 2
Nucleotide sequence of the afimbrial-adhesin-encoding afa-3 gene cluster and its translocation via flanking IS1 insertion sequences
Garcia, M.I., Labigne, A., Le Bouguenec, C.
J. Bacteriol. (1994), 176, 7601-7613, PubMed 8002584 , DOI 10.1128/jb.176.24.7601-7613.1994 ,
Entries sharing articles Swiss-Prot: 7 entries Detail
  Swiss-Prot: Q47036 released on 1997-11-01
    Title INTL_ECOLX Entity Probable site-specific recombinase in afa region
  Swiss-Prot: Q47037 released on 1997-11-01
    Title AFAF_ECOLX Entity Dr hemagglutinin AFA-III operon regulatory protein AfaF
  Swiss-Prot: Q47038 released on 1997-11-01
    Title AFAD_ECOLX Entity Protein AfaD
  Swiss-Prot: Q57254 released on 1997-11-01
    Title AFAE3_ECOLX Entity Afimbrial adhesin AFA-III
  Swiss-Prot: P53515 released on 1996-10-01
    Title AFAA_ECOLX Entity AFA-III adhesin operon regulatory protein
  Swiss-Prot: P53516 released on 1996-10-01
    Title AFAB_ECOLX Entity Chaperone protein AfaB
  Swiss-Prot: P53517 released on 1996-10-01
    Title AFAC_ECOLX Entity Outer membrane usher protein AfaC
Related entities 1. afaD-dsc, : 1 : 1 : 8 entities Detail
More details for 10 related entities
Experiments performed 1 experiments Detail
nullKeywords afaD, integrin, afimbrial sheath, DAEC, NMR