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Found 1 Document (3.112 seconds)

BMRB: 5948

1Q7J

Structure Analysis of Integrin alpha IIb beta 3 - Specific Disintegrin with the AKGDWN Motif

Authors

Chuang, W., Chen, C., Shiu, J., Liu, Y.

Assembly

Disintegrin kistrin, AKGDWN Mutant

Entity

1. Disintegrin kistrin, AKGDWN Mutant (polymer, Thiol state: all disulfide bound), 68 monomers, 7348.221 Da Detail

GKECDCSSPE NPCCDAATCK LRPGAQCGEG LCCEQCKFSR AGKICRIAKG DWNDDRCTGQ SADCPRYH

Formula weight

7348.221 Da

Entity Connection

disulfide 6 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS4:SG	1:CYS19:SG
2	disulfide	sing	1:CYS6:SG	1:CYS14:SG
3	disulfide	sing	1:CYS27:SG	1:CYS33:SG
4	disulfide	sing	1:CYS13:SG	1:CYS36:SG
5	disulfide	sing	1:CYS32:SG	1:CYS57:SG
6	disulfide	sing	1:CYS45:SG	1:CYS64:SG

Source organism

Calloselasma rhodostoma

Exptl. method

NMR

Refine. method

Hybrid distance geometry-dynamical simulated annealing method

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 91.1 %, Completeness (bb): 99.5 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹⁵N
All	91.1 % (409 of 449)	91.0 % (345 of 379)	91.4 % (64 of 70)
Backbone	99.5 % (202 of 203)	99.3 % (138 of 139)	100.0 % (64 of 64)
Sidechain	84.1 % (207 of 246)	86.3 % (207 of 240)	0.0 % (0 of 6)
Aromatic	66.7 % (12 of 18)	70.6 % (12 of 17)	0.0 % (0 of 1)
Methyl	93.8 % (15 of 16)	93.8 % (15 of 16)

1. Rhodostomin

GKECDCSSPE NPCCDAATCK LRPGAQCGEG LCCEQCKFSR AGKICRIAKG DWNDDRCTGQ SADCPRYH

Show sample condition

Sample

Pressure 1 atm, Temperature 300 (±0.01) K, pH 6.0 (±0.01)

#	Name	Concentration
1	Rhodostomin	3 mM
2	H2O	90 %
3	D2O	10 %

Protein Blocks Logo

Calculated from 20 models in PDB: 1Q7J, Strand ID: A Detail

Release date

2006-01-11

Citation 1

Structure Analysis of Integrin alpha IIb beta 3 - Specific Disintegrin with the AKGDWN Motif
Chuang, W., Chen, C., Shiu, J., Liu, Y.

Show more 1 citaion

Citation 2

Expression in Pichia pastoris and characterization by circular dichroism and NMR of rhodostomin
Guo, R.T., Chou, L.J., Chen, Y.C., Chen, C.Y., Pari, K., Jen, C.J., Lo, S.J., Huang, S.L., Lee, C.Y., Chang, T.W., Chaung, W.J.
Proteins (2001), 43, 499-508, PubMed 11340665 , DOI: , Abstract

Hide non-primary 1 citaion

Entries sharing articles BMRB: 2 entries Detail

  BMRB: 5949 released on 2006-01-11
    Title Structure Analysis of Integrin alpha IIb beta 3 - Specific Disintegrin with the AKGDWN Motif
  BMRB: 5117 released on 2003-04-08
    Title Structural and Dynamic Differences of Rhodostomin, an RGD-containing Disintegrin, and its D51E mutant

Related entities 1. Disintegrin kistrin, AKGDWN Mutant, : 1 : 2 : 234 entities Detail

Experiments performed 6 experiments Detail

1 2D NOESY

Instrument

Model Bruker AVANCE (600 MHz)

Sample #1

Pressure 1 atm, Temperature 300 (±0.01) K, pH 6.0 (±0.01)

#	Name	Concentration
1	Rhodostomin	3 mM
2	H2O	90 %
3	D2O	10 %

Sample #2

Pressure 1 atm, Temperature 300 (±0.01) K, pH 6.0 (±0.01)

#	Name	Isotope labeling	Type	Concentration
4	Rhodostomin			3 mM
5	D2O			100 %

Sample #3

Pressure 1 atm, Temperature 300 (±0.01) K, pH 6.0 (±0.01)

#	Name	Isotope labeling	Concentration
6	Rhodostomin	[U-15N]	2.5 mM
7	H2O		90 %
8	D2O		10 %

2 2D TOCSY

Instrument

Model Bruker AVANCE (600 MHz)

Sample #1

Pressure 1 atm, Temperature 300 (±0.01) K, pH 6.0 (±0.01)

#	Name	Concentration
1	Rhodostomin	3 mM
2	H2O	90 %
3	D2O	10 %

Sample #2

Pressure 1 atm, Temperature 300 (±0.01) K, pH 6.0 (±0.01)

#	Name	Isotope labeling	Type	Concentration
4	Rhodostomin			3 mM
5	D2O			100 %

Sample #3

Pressure 1 atm, Temperature 300 (±0.01) K, pH 6.0 (±0.01)

#	Name	Isotope labeling	Concentration
6	Rhodostomin	[U-15N]	2.5 mM
7	H2O		90 %
8	D2O		10 %

3 3D 15N-separated NOESY

Instrument

Model Bruker AVANCE (600 MHz)

Sample #1

Pressure 1 atm, Temperature 300 (±0.01) K, pH 6.0 (±0.01)

#	Name	Concentration
1	Rhodostomin	3 mM
2	H2O	90 %
3	D2O	10 %

Sample #2

Pressure 1 atm, Temperature 300 (±0.01) K, pH 6.0 (±0.01)

#	Name	Isotope labeling	Type	Concentration
4	Rhodostomin			3 mM
5	D2O			100 %

Sample #3

Pressure 1 atm, Temperature 300 (±0.01) K, pH 6.0 (±0.01)

#	Name	Isotope labeling	Concentration
6	Rhodostomin	[U-15N]	2.5 mM
7	H2O		90 %
8	D2O		10 %

4 3D 15N-separated TOCSY

Instrument

Model Bruker AVANCE (600 MHz)

Sample #1

Pressure 1 atm, Temperature 300 (±0.01) K, pH 6.0 (±0.01)

#	Name	Concentration
1	Rhodostomin	3 mM
2	H2O	90 %
3	D2O	10 %

Sample #2

Pressure 1 atm, Temperature 300 (±0.01) K, pH 6.0 (±0.01)

#	Name	Isotope labeling	Type	Concentration
4	Rhodostomin			3 mM
5	D2O			100 %

Sample #3

Pressure 1 atm, Temperature 300 (±0.01) K, pH 6.0 (±0.01)

#	Name	Isotope labeling	Concentration
6	Rhodostomin	[U-15N]	2.5 mM
7	H2O		90 %
8	D2O		10 %

5 HNHA

Instrument

Model Bruker AVANCE (600 MHz)

Sample #1

Pressure 1 atm, Temperature 300 (±0.01) K, pH 6.0 (±0.01)

#	Name	Concentration
1	Rhodostomin	3 mM
2	H2O	90 %
3	D2O	10 %

Sample #2

Pressure 1 atm, Temperature 300 (±0.01) K, pH 6.0 (±0.01)

#	Name	Isotope labeling	Type	Concentration
4	Rhodostomin			3 mM
5	D2O			100 %

Sample #3

Pressure 1 atm, Temperature 300 (±0.01) K, pH 6.0 (±0.01)

#	Name	Isotope labeling	Concentration
6	Rhodostomin	[U-15N]	2.5 mM
7	H2O		90 %
8	D2O		10 %

6 15N-HSQC

Instrument

Model Bruker AVANCE (600 MHz)

Sample #1

Pressure 1 atm, Temperature 300 (±0.01) K, pH 6.0 (±0.01)

#	Name	Concentration
1	Rhodostomin	3 mM
2	H2O	90 %
3	D2O	10 %

Sample #2

Pressure 1 atm, Temperature 300 (±0.01) K, pH 6.0 (±0.01)

#	Name	Isotope labeling	Type	Concentration
4	Rhodostomin			3 mM
5	D2O			100 %

Sample #3

Pressure 1 atm, Temperature 300 (±0.01) K, pH 6.0 (±0.01)

#	Name	Isotope labeling	Concentration
6	Rhodostomin	[U-15N]	2.5 mM
7	H2O		90 %
8	D2O		10 %

nullKeywords disintegrin, integrin, NMR, rhodostomin

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Found 1 Document (3.112 seconds)

BMRB: 5948

Sample

Entries sharing articles BMRB: 2 entries Detail

Related entities 1. Disintegrin kistrin, AKGDWN Mutant, : 1 : 2 : 234 entities Detail

Experiments performed 6 experiments Detail

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