BMRB: 5950

1H, 15N and 13C resonance assignment for the Escherichia coli cell division activator CedA

Authors

Chen, H.A., Simpson, P.J., Matthews, S.J.

Assembly

Cell division Activator CedA

Entity

1. Cell division Activator CedA (polymer, Thiol state: not present), 87 monomers, 10200.78 Da Detail

MRLVKPVMKK PLRQQNRQII SYVPRTEPAP PEHAIKMDSF RDVWMLRGKY VAFVLMGESF LRSPAFTVPE SAQRWANQIR QEGEVTE

Formula weight

10200.78 Da

Source organism

Escherichia coli

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 92.7 %, Completeness (bb): 93.9 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	92.7 % (998 of 1077)	94.1 % (538 of 572)	90.1 % (375 of 416)	95.5 % (85 of 89)
Backbone	93.9 % (475 of 506)	97.0 % (164 of 169)	91.1 % (235 of 258)	96.2 % (76 of 79)
Sidechain	92.4 % (605 of 655)	92.8 % (374 of 403)	91.7 % (222 of 242)	90.0 % (9 of 10)
Aromatic	81.0 % (68 of 84)	81.0 % (34 of 42)	80.0 % (32 of 40)	100.0 % (2 of 2)
Methyl	95.3 % (82 of 86)	97.7 % (42 of 43)	93.0 % (40 of 43)

1. Cell division activator

MRLVKPVMKK PLRQQNRQII SYVPRTEPAP PEHAIKMDSF RDVWMLRGKY VAFVLMGESF LRSPAFTVPE SAQRWANQIR QEGEVTE

Show sample condition

Sample

Temperature 303 (±1) K, pH 3.8 (±0.3)

#	Name	Isotope labeling	Type	Concentration
1	Cell division activator			0.5 mM

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LACS Plot; CA

Referencing offset: 0.0 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.0 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.04 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.27 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 1 models in PDB: 2BN8, Strand ID: A Detail

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Release date

2005-05-22

Citation

Solution structure and interactions of the Escherichia coli cell division activator protein CedA
Chen, H.A., Simpson, P.J., Huyton, T., Roper, D., Matthews, S.J.
Biochemistry (2005), 44, 6738-6744, PubMed 15865419 , DOI 10.1021/bi0500269 , Abstract

Related entities 1. Cell division Activator CedA, : 1 : 3 : 5 entities Detail