BMRBj - BMREntryMaster

Found 1 Document (3.092 seconds)

BMRB: 5956

1R05

NMR solution structure of Max in absence of DNA: Insight into the mechanism of DNA recognition by B-HLH-LZ transcription factors

Authors

Sauve, S., Tremblay, L., Lavigne, P.

Assembly

Max*VL homodimer

Entity

1. Max*VL homodimer (polymer, Thiol state: all disulfide bound), 87 monomers, 10151.34 × 2 Da Detail

MADKRAHHNA LERKRRDHIK DSFHSLRDSV PSLQGEKASR AQILDKATEY IQYMRRKVHT LQQDIDDLKR QNALLEQQVR ALEGSGC

Total weight

20302.68 Da

Max. entity weight

10151.34 Da

Entity Connection

disulfide 1 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS87:SG	1:CYS87:SG

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 90.8 %, Completeness: 64.0 %, Completeness (bb): 80.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	64.0 % (661 of 1033)	61.4 % (337 of 549)	63.9 % (248 of 388)	79.2 % (76 of 96)
Backbone	80.4 % (418 of 520)	80.7 % (142 of 176)	79.8 % (206 of 258)	81.4 % (70 of 86)
Sidechain	51.8 % (309 of 597)	52.3 % (195 of 373)	50.5 % (108 of 214)	60.0 % (6 of 10)
Aromatic	30.4 % (14 of 46)	34.8 % (8 of 23)	26.1 % (6 of 23)
Methyl	70.2 % (59 of 84)	78.6 % (33 of 42)	61.9 % (26 of 42)

1. Max

MADKRAHHNA LERKRRDHIK DSFHSLRDSV PSLQGEKASR AQILDKATEY IQYMRRKVHT LQQDIDDLKR QNALLEQQVR ALEGSGC

Show sample condition

Sample #1

Temperature 308 K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
1	Max	[U-15N]		30 mM

Sample #2

Temperature 308 K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
2	Max	[U-13C; U-15N]		30 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.0
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.0

Referencing offset: -0.82 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.0
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.0

Referencing offset: -0.82 ppm, Outliers: 3 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.0
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.0

Referencing offset: 0.16 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.0
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.0

Referencing offset: -0.84 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 6 models in PDB: 1R05, Strand ID: A, B Detail

Release date

2003-09-18

Citation 1

The NMR solution structure of a mutant of the Max b/HLH/LZ free of DNA: insights into the specific and reversible DNA binding mechanism of dimeric transcription factors
Sauve, S., Tremblay, L., Lavigne, P.
J. Mol. Biol. (2004), 342, 813-832, PubMed 15342239 , DOI 10.1016/j.jmb.2004.07.058 , Abstract

Show more 1 citaion

Citation 2

Improving the thermodynamic stability of the leucine zipper of max increases the stability of its b-HLH-LZ:E-box complex
Jean-Francois, N., Frederic, G., Raymund, W., Benoit, C., Lavigne, P.
J. Mol. Biol. (2003), 326, 1577-1595, PubMed 12595267 , DOI: , Abstract

Hide non-primary 1 citaion

Related entities 1. Max*VL homodimer, : 1 : 14 : 166 entities Detail

Interaction partners 1. Max*VL homodimer, : 87 interactors Detail

More details for 87 interactors identified by 20 citations

Experiments performed 11 experiments Detail

1 15N HSQC

Instrument

Model Varian UNITY-INOVA (600 MHz)

Sample #1

Temperature 308 K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
1	Max	[U-15N]		30 mM

Sample #2

Temperature 308 K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
2	Max	[U-13C; U-15N]		30 mM

2 13C HSQC

Instrument

Model Varian UNITY-INOVA (600 MHz)

Sample #1

Temperature 308 K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
1	Max	[U-15N]		30 mM

Sample #2

Temperature 308 K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
2	Max	[U-13C; U-15N]		30 mM

3 15N NOESY HSQC

Instrument

Model Varian UNITY-INOVA (600 MHz)

Sample #1

Temperature 308 K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
1	Max	[U-15N]		30 mM

Sample #2

Temperature 308 K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
2	Max	[U-13C; U-15N]		30 mM

4 13C NOESY HSQC

Instrument

Model Varian UNITY-INOVA (600 MHz)

Sample #1

Temperature 308 K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
1	Max	[U-15N]		30 mM

Sample #2

Temperature 308 K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
2	Max	[U-13C; U-15N]		30 mM

5 13C aromatic NOESY HSQC

Instrument

Model Varian UNITY-INOVA (600 MHz)

Sample #1

Temperature 308 K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
1	Max	[U-15N]		30 mM

Sample #2

Temperature 308 K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
2	Max	[U-13C; U-15N]		30 mM

6 HNCA

Instrument

Model Varian UNITY-INOVA (600 MHz)

Sample #1

Temperature 308 K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
1	Max	[U-15N]		30 mM

Sample #2

Temperature 308 K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
2	Max	[U-13C; U-15N]		30 mM

7 HN(CO)CA

Instrument

Model Varian UNITY-INOVA (600 MHz)

Sample #1

Temperature 308 K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
1	Max	[U-15N]		30 mM

Sample #2

Temperature 308 K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
2	Max	[U-13C; U-15N]		30 mM

8 HNCACB

Instrument

Model Varian UNITY-INOVA (600 MHz)

Sample #1

Temperature 308 K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
1	Max	[U-15N]		30 mM

Sample #2

Temperature 308 K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
2	Max	[U-13C; U-15N]		30 mM

9 CBCA(CO)NH

Instrument

Model Varian UNITY-INOVA (600 MHz)

Sample #1

Temperature 308 K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
1	Max	[U-15N]		30 mM

Sample #2

Temperature 308 K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
2	Max	[U-13C; U-15N]		30 mM

10 HCCH TOCSY

Instrument

Model Varian UNITY-INOVA (600 MHz)

Sample #1

Temperature 308 K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
1	Max	[U-15N]		30 mM

Sample #2

Temperature 308 K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
2	Max	[U-13C; U-15N]		30 mM

11 HCCH COSY

Instrument

Model Varian UNITY-INOVA (600 MHz)

Sample #1

Temperature 308 K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
1	Max	[U-15N]		30 mM

Sample #2

Temperature 308 K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
2	Max	[U-13C; U-15N]		30 mM

null

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Found 1 Document (3.092 seconds)

BMRB: 5956

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Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. Max*VL homodimer, : 1 : 14 : 166 entities Detail

Interaction partners 1. Max*VL homodimer, : 87 interactors Detail

Experiments performed 11 experiments Detail

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