BMRBj - BMREntryMaster

Found 1 Document (2.28 seconds)

BMRB: 5958

1V49

1H, 13C and 15N resonance assignments of human microtubule-associated protein light chain-3

Authors

Kouno, T., Mizuguchi, M., Tanida, I., Ueno, T., Kominami, E., Kawano, K.

Assembly

MAP-LC3 monomer

Entity

1. MAP-LC3 monomer (polymer, Thiol state: not present), 120 monomers, 14129.11 Da Detail

MPSEKTFKQR RTFEQRVEDV RLIREQHPTK IPVIIERYKG EKQLPVLDKT KFLVPDHVNM SELIKIIRRR LQLNANQAFF LLVNGHSMVS VSTPISEVYE SEKDEDGFLY MVYASQETFG

Formula weight

14129.11 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

distance geometry, simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.2 %, Completeness: 40.7 %, Completeness (bb): 82.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	40.7 % (606 of 1488)	31.8 % (249 of 784)	42.1 % (244 of 579)	90.4 % (113 of 125)
Backbone	82.9 % (587 of 708)	98.7 % (235 of 238)	67.1 % (239 of 356)	99.1 % (113 of 114)
Sidechain	2.2 % (20 of 896)	1.6 % (9 of 546)	3.2 % (11 of 339)	0.0 % (0 of 11)
Aromatic	0.0 % (0 of 114)	0.0 % (0 of 57)	0.0 % (0 of 57)
Methyl	7.5 % (10 of 134)	7.5 % (5 of 67)	7.5 % (5 of 67)

1. microtubule-associated protein light chain-3

MPSEKTFKQR RTFEQRVEDV RLIREQHPTK IPVIIERYKG EKQLPVLDKT KFLVPDHVNM SELIKIIRRR LQLNANQAFF LLVNGHSMVS VSTPISEVYE SEKDEDGFLY MVYASQETFG

Show sample condition

Sample

Temperature 298 (±0.5) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	microtubule-associated protein light chain-3	[U-13C; U-15N]		0.8 ~ 1.0 mM

Protein Blocks Logo

Calculated from 1 models in PDB: 1V49, Strand ID: A Detail

Release date

2004-07-05

Citation

1H, 13C, and 15N resonance assignments of human microtubule-associated protein light chain-3
Kouno, T., Mizuguchi, M., Tanida, I., Ueno, T., Kominami, E., Kawano, K.
J. Biomol. NMR (2004), 29, 415-416, PubMed 15213446 , DOI 10.1023/B:JNMR.0000032505.99071.ea , Abstract

Related entities 1. MAP-LC3 monomer, : 1 : 6 : 13 : 21 : 208 entities Detail

Interaction partners 1. MAP-LC3 monomer, : 110 interactors Detail

More details for 110 interactors identified by 25 citations

Experiments performed 9 experiments Detail

1 1H,15N-HSQC

Instrument

Model Bruker DMX (500 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	microtubule-associated protein light chain-3	[U-13C; U-15N]		0.8 ~ 1.0 mM

Sample #2

Temperature 298 (±0.5) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	microtubule-associated protein light chain-3			0.8 ~ 1.0 mM

2 HNCA

Instrument

Model Bruker DMX (500 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	microtubule-associated protein light chain-3	[U-13C; U-15N]		0.8 ~ 1.0 mM

Sample #2

Temperature 298 (±0.5) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	microtubule-associated protein light chain-3			0.8 ~ 1.0 mM

3 CBCANH

Instrument

Model Bruker DMX (500 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	microtubule-associated protein light chain-3	[U-13C; U-15N]		0.8 ~ 1.0 mM

Sample #2

Temperature 298 (±0.5) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	microtubule-associated protein light chain-3			0.8 ~ 1.0 mM

4 CBCA(CO)NH

Instrument

Model Bruker DMX (500 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	microtubule-associated protein light chain-3	[U-13C; U-15N]		0.8 ~ 1.0 mM

Sample #2

Temperature 298 (±0.5) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	microtubule-associated protein light chain-3			0.8 ~ 1.0 mM

5 HNCO

Instrument

Model Bruker DMX (500 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	microtubule-associated protein light chain-3	[U-13C; U-15N]		0.8 ~ 1.0 mM

Sample #2

Temperature 298 (±0.5) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	microtubule-associated protein light chain-3			0.8 ~ 1.0 mM

6 HN(CA)CO

Instrument

Model Bruker DMX (500 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	microtubule-associated protein light chain-3	[U-13C; U-15N]		0.8 ~ 1.0 mM

Sample #2

Temperature 298 (±0.5) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	microtubule-associated protein light chain-3			0.8 ~ 1.0 mM

7 H(COO)NH

Instrument

Model Bruker DMX (500 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	microtubule-associated protein light chain-3	[U-13C; U-15N]		0.8 ~ 1.0 mM

Sample #2

Temperature 298 (±0.5) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	microtubule-associated protein light chain-3			0.8 ~ 1.0 mM

8 HBHA(CBCA)NH

Instrument

Model Bruker DMX (500 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	microtubule-associated protein light chain-3	[U-13C; U-15N]		0.8 ~ 1.0 mM

Sample #2

Temperature 298 (±0.5) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	microtubule-associated protein light chain-3			0.8 ~ 1.0 mM

9 HBHA(CBCACO)NH

Instrument

Model Bruker DMX (500 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	microtubule-associated protein light chain-3	[U-13C; U-15N]		0.8 ~ 1.0 mM

Sample #2

Temperature 298 (±0.5) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	microtubule-associated protein light chain-3			0.8 ~ 1.0 mM

null

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Found 1 Document (2.28 seconds)

BMRB: 5958

Sample

Related entities 1. MAP-LC3 monomer, : 1 : 6 : 13 : 21 : 208 entities Detail

Interaction partners 1. MAP-LC3 monomer, : 110 interactors Detail

Experiments performed 9 experiments Detail

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