BMRBj - BMREntryMaster

Found 1 Document (1.411 seconds)

BMRB: 5961

2FYJ

Binding site structure of one LRP/RAP complex - implications for a common ligand/receptor binding motif

Authors

Jensen, G.A., Andersen, O.M., Bonvin, A.M.J.J., Bjerrum-Bohr, I., Etzeroth, M., Thoegersen, H.C., Poulsen, F.M., Kragelund, B.B.

Assembly

complement type repeats 5 and 6 from LRP

Entity

1. complement type repeats 5 and 6 (polymer, Thiol state: all disulfide bound), 82 monomers, 8938.567 Da Detail

SARTCPPNQF SCASGRCIPI SWTCDLDDDC GDRSDESASC AYPTCFPLTQ FTCNNGRCIN INWRCDNDND CGDNSDEAGC SH

2. CA (non-polymer), 40.078 × 2 Da

Total weight

9018.724 Da

Max. entity weight

8938.567 Da

Entity Connection

disulfide 6 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS5:SG	1:CYS17:SG
2	disulfide	sing	1:CYS12:SG	1:CYS30:SG
3	disulfide	sing	1:CYS24:SG	1:CYS40:SG
4	disulfide	sing	1:CYS45:SG	1:CYS58:SG
5	disulfide	sing	1:CYS53:SG	1:CYS71:SG
6	disulfide	sing	1:CYS65:SG	1:CYS80:SG

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

SIMMULATED ANNEALING USING XPLOR-NIH, REFINEMENT IN EXPLICIT WATER LAYER USING CNS

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.3 %, Completeness: 89.9 %, Completeness (bb): 92.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹⁵N
All	89.9 % (473 of 526)	89.7 % (392 of 437)	91.0 % (81 of 89)
Backbone	92.1 % (222 of 241)	93.3 % (153 of 164)	89.6 % (69 of 77)
Sidechain	88.1 % (251 of 285)	87.5 % (239 of 273)	100.0 % (12 of 12)
Aromatic	74.3 % (26 of 35)	72.7 % (24 of 33)	100.0 % (2 of 2)
Methyl	100.0 % (22 of 22)	100.0 % (22 of 22)

1. complement type repeats 5 and 6

SARTCPPNQF SCASGRCIPI SWTCDLDDDC GDRSDESASC AYPTCFPLTQ FTCNNGRCIN INWRCDNDND CGDNSDEAGC SH

Show sample condition

Sample #1

Temperature 298 (±1) K, pH 7.0 (±0.1)

#	Name	Concentration
1	complement type repeats 5 and 6	0.5 mM
2	CaCl2	10 mM
3	MgCl2	1 mM
4	NaCl	100 mM

Sample #2

Temperature 298 (±1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
5	complement type repeats 5 and 6	[U-99% 15N]	0.5 mM
6	CaCl2		10 mM
7	MgCl2		1 mM
8	NaCl		100 mM

Protein Blocks Logo

Calculated from 15 models in PDB: 2FYJ, Strand ID: A Detail

Release date

2003-09-30

Citation 1

Binding site structure of one LRP-RAP complex: implications for a common ligand-receptor binding motif
Jensen, G.A., Andersen, O.M., Bonvin, A.M.J.J., Bjerrum-Bohr, I., Etzerodt, M., Thoegersen, H.C., O'Shea, C., Poulsen, F.M., Kragelund, B.B.
J. Mol. Biol. (2006), 362, 700-716, PubMed 16938309 , DOI 10.1016/j.jmb.2006.07.013 , Abstract

Show more 2 citaions

Citation 2

Identification of the minimal functional unit in the low density lipoprotein receptor-related protein for binding the receptor-associated protein (RAP). A conserved acidic residue in the complement-type repeats is important for recognition of RAP
Andersen, O.M., Christensen, L.L., Christensen, P.A., Sorensen, E.S., Jacobsen, C., Moestrup, S.K., Etzerodt, M., Thogersen, H.C.
J. Biol. Chem. (2000), 275, 21017-21024, PubMed 10747921 , DOI 10.1074/jbc.M000507200 , Abstract

Citation 3

The solution structure of the N-terminal domain of alpha2-macroglobulin receptor-associated protein
Nielsen, P.R., Ellgaard, L., Etzerodt, M., Thogersen, H.C., Poulsen, F.M.
Proc. Natl. Acad. Sci. U. S. A. (1997), 94, 7521-7525, PubMed 9207124 , DOI: , Abstract

Hide non-primary 2 citaions

Related entities 1. complement type repeats 5 and 6, : 1 : 3 : 1 : 835 entities Detail

Interaction partners 1. complement type repeats 5 and 6, : 23 interactors Detail

More details for 23 interactors identified by 14 citations

Experiments performed 6 experiments Detail

1 DQF-COSY

Instrument

#1 Model Varian UNITY (750 MHz)

#2 Model Varian UNITY (800 MHz)

Sample #1

Temperature 298 (±1) K, pH 7.0 (±0.1)

#	Name	Concentration
1	complement type repeats 5 and 6	0.5 mM
2	CaCl2	10 mM
3	MgCl2	1 mM
4	NaCl	100 mM

Sample #2

Temperature 298 (±1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
5	complement type repeats 5 and 6	[U-99% 15N]	0.5 mM
6	CaCl2		10 mM
7	MgCl2		1 mM
8	NaCl		100 mM

2 NOESY

Instrument

#1 Model Varian UNITY (750 MHz)

#2 Model Varian UNITY (800 MHz)

Sample #1

Temperature 298 (±1) K, pH 7.0 (±0.1)

#	Name	Concentration
1	complement type repeats 5 and 6	0.5 mM
2	CaCl2	10 mM
3	MgCl2	1 mM
4	NaCl	100 mM

Sample #2

Temperature 298 (±1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
5	complement type repeats 5 and 6	[U-99% 15N]	0.5 mM
6	CaCl2		10 mM
7	MgCl2		1 mM
8	NaCl		100 mM

3 TOCSY

Instrument

#1 Model Varian UNITY (750 MHz)

#2 Model Varian UNITY (800 MHz)

Sample #1

Temperature 298 (±1) K, pH 7.0 (±0.1)

#	Name	Concentration
1	complement type repeats 5 and 6	0.5 mM
2	CaCl2	10 mM
3	MgCl2	1 mM
4	NaCl	100 mM

Sample #2

Temperature 298 (±1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
5	complement type repeats 5 and 6	[U-99% 15N]	0.5 mM
6	CaCl2		10 mM
7	MgCl2		1 mM
8	NaCl		100 mM

4 15N-HSQC

Instrument

#1 Model Varian UNITY (750 MHz)

#2 Model Varian UNITY (800 MHz)

Sample #1

Temperature 298 (±1) K, pH 7.0 (±0.1)

#	Name	Concentration
1	complement type repeats 5 and 6	0.5 mM
2	CaCl2	10 mM
3	MgCl2	1 mM
4	NaCl	100 mM

Sample #2

Temperature 298 (±1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
5	complement type repeats 5 and 6	[U-99% 15N]	0.5 mM
6	CaCl2		10 mM
7	MgCl2		1 mM
8	NaCl		100 mM

5 15N-NOESY-HSQC

Instrument

#1 Model Varian UNITY (750 MHz)

#2 Model Varian UNITY (800 MHz)

Sample #1

Temperature 298 (±1) K, pH 7.0 (±0.1)

#	Name	Concentration
1	complement type repeats 5 and 6	0.5 mM
2	CaCl2	10 mM
3	MgCl2	1 mM
4	NaCl	100 mM

Sample #2

Temperature 298 (±1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
5	complement type repeats 5 and 6	[U-99% 15N]	0.5 mM
6	CaCl2		10 mM
7	MgCl2		1 mM
8	NaCl		100 mM

6 15N-TOCSY-HSQC

Instrument

#1 Model Varian UNITY (750 MHz)

#2 Model Varian UNITY (800 MHz)

Sample #1

Temperature 298 (±1) K, pH 7.0 (±0.1)

#	Name	Concentration
1	complement type repeats 5 and 6	0.5 mM
2	CaCl2	10 mM
3	MgCl2	1 mM
4	NaCl	100 mM

Sample #2

Temperature 298 (±1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
5	complement type repeats 5 and 6	[U-99% 15N]	0.5 mM
6	CaCl2		10 mM
7	MgCl2		1 mM
8	NaCl		100 mM

nullKeywords CR56, HADDOCK, NMR, RAP, receptor, recognition helix

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Found 1 Document (1.411 seconds)

BMRB: 5961

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Related entities 1. complement type repeats 5 and 6, : 1 : 3 : 1 : 835 entities Detail

Interaction partners 1. complement type repeats 5 and 6, : 23 interactors Detail

Experiments performed 6 experiments Detail

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