BMRB: 5963

1H, 13C, 15N resonance assignment of hypothetical protein hi1723 from Haemophilus Influenzae

Authors

Yeh, D., Orban, J.

Assembly

hypothetical protein hi1723

Entity

1. hypothetical protein hi1723 (polymer, Thiol state: all free), 114 monomers, 12193.48 Da Detail

MIDDMAVPLT FTDAAANKVK SLISEEENTD LKLRVYITGG GCSGFQYGFT FDEKVNDGDL TIEKSGVQLV IDPMSLQYLI GGTVDYTEGL EGSRFTVNNP NATSTCGCGS SFSI

Formula weight

12193.48 Da

Source organism

Haemophilus influenzae

Exptl. method

NMR

Refine. method

CNS 1.1

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 88.5 %, Completeness (bb): 94.1 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	88.5 % (1103 of 1247)	94.2 % (599 of 636)	78.6 % (386 of 491)	98.3 % (118 of 120)
Backbone	94.1 % (638 of 678)	97.5 % (232 of 238)	90.3 % (297 of 329)	98.2 % (109 of 111)
Sidechain	83.7 % (561 of 670)	92.2 % (367 of 398)	70.3 % (185 of 263)	100.0 % (9 of 9)
Aromatic	56.5 % (52 of 92)	84.8 % (39 of 46)	28.3 % (13 of 46)
Methyl	93.0 % (119 of 128)	100.0 % (64 of 64)	85.9 % (55 of 64)

1. hypothetical protein hi1723

MIDDMAVPLT FTDAAANKVK SLISEEENTD LKLRVYITGG GCSGFQYGFT FDEKVNDGDL TIEKSGVQLV IDPMSLQYLI GGTVDYTEGL EGSRFTVNNP NATSTCGCGS SFSI

Show sample condition

Sample

Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
1	hypothetical protein hi1723	[U-95% 13C; U-90% 15N]		1.0 mM

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LACS Plot; CA

Referencing offset: 1.73 ppm, Outliers: 4 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	H2O	protons	0.0 ppm	null	indirect	0.2514495
1H	H2O	protons	4.75 ppm	internal	direct	1.0
15N	H2O	protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 1.73 ppm, Outliers: 4 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	H2O	protons	0.0 ppm	null	indirect	0.2514495
1H	H2O	protons	4.75 ppm	internal	direct	1.0
15N	H2O	protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.08 ppm, Outliers: 4 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	H2O	protons	0.0 ppm	null	indirect	0.2514495
1H	H2O	protons	4.75 ppm	internal	direct	1.0
15N	H2O	protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.17 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	H2O	protons	0.0 ppm	null	indirect	0.2514495
1H	H2O	protons	4.75 ppm	internal	direct	1.0
15N	H2O	protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2APN, Strand ID: A Detail

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Release date

2004-04-06

Citation 1

NMR assignment of HI1723 from Haemophilus influenzae--a sequence homologue from the iron sulfur cluster assembly (IscA) family
Yeh, D., Liu, F., Bonander, N., Eisenstein, E., Orban, J.
J. Biomol. NMR (2004), 29, 213-214, PubMed 15014238 , DOI 10.1023/B:JNMR.0000019242.88149.c5 , Abstract

Show more 1 citaion

Citation 2

Whole-genome random sequencing and assembly of Haemophilus influenzae Rd
Fleischmann, R.D., Adams, M.D., White, O., Clayton, R.A., Kirkness, E.F., Kerlavage, A.R., Bult, C.J., Tomb, J.F., Dougherty, B.A., Merrick, J.M.
Science (1995), 269, 496-512, PubMed 7542800 , DOI: , Abstract

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Related entities 1. hypothetical protein hi1723, : 1 : 1 : 2 : 147 entities Detail