BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	90.4 % (767 of 848)	92.0 % (414 of 450)	86.7 % (287 of 331)	98.5 % (66 of 67)
Backbone	97.5 % (388 of 398)	97.0 % (131 of 135)	97.5 % (195 of 200)	98.4 % (62 of 63)
Sidechain	83.7 % (430 of 514)	87.0 % (274 of 315)	77.9 % (152 of 195)	100.0 % (4 of 4)
Aromatic	48.6 % (35 of 72)	94.4 % (34 of 36)	0.0 % (0 of 35)	100.0 % (1 of 1)
Methyl	100.0 % (68 of 68)	100.0 % (34 of 34)	100.0 % (34 of 34)

1. dematin

PGLQIYPYEM LVVTNKGRTK LPPGVDRMRL ERHLSAEDFS RVFAMSPEEF GKLALWKRNE LKKKASLF

Show sample condition

Sample

Pressure 1 atm, Temperature 293 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	dematin	[U-13C; U-15N]	1 mM
2	phosphat		10 mM
3	TMSP		0.5 mM
4	sodium azide		0.01 %
5	D2O		10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type
¹H	na	na	null	null
¹⁵N	na	na	null	null
¹³C	na	na	null	null

Referencing offset: -0.1 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type
¹H	na	na	null	null
¹⁵N	na	na	null	null
¹³C	na	na	null	null

Referencing offset: -0.1 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type
¹H	na	na	null	null
¹⁵N	na	na	null	null
¹³C	na	na	null	null

Referencing offset: 0.03 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type
¹H	na	na	null	null
¹⁵N	na	na	null	null
¹³C	na	na	null	null

Referencing offset: 1.13 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 13 models in PDB: 1QZP, Strand ID: A Detail

Release date

2004-02-11

Citation

The NMR structure of dematin headpiece reveals a dynamic loop that is conformationally altered upon phosphorylation at a distal site
Frank, B.S., Vardar, D., Chishti, A.H., McKnight, C.J.
J. Biol. Chem. (2004), 279, 7909-7916, PubMed 14660664 , DOI 10.1074/jbc.M310524200 , Abstract

Related entities 1. dematin, : 1 : 1 : 107 entities Detail

Interaction partners 1. dematin, : 7 interactors Detail

More details for 7 interactors identified by 2 citations

Experiments performed 10 experiments Detail

1 3D 13C-separated NOESY

Instrument

Model Bruker DMX (500 MHz)

Sample #1

Pressure 1 atm, Temperature 293 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	dematin	[U-13C; U-15N]	1 mM
2	phosphat		10 mM
3	TMSP		0.5 mM
4	sodium azide		0.01 %
5	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 293 K, pH 6.0

#	Name	Isotope labeling	Concentration
6	dematin	[U-15N]	4 mM
7	phosphat		10 mM
8	TMSP		0.5 mM
9	sodium azide		0.01 %
10	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 293 K, pH 6.0

#	Name	Isotope labeling	Concentration
11	dematin	[U-10% 13C; U-15N]	0.7 mM
12	phosphat		10 mM
13	TMSP		0.5 mM
14	sodium azide		0.01 %
15	D2O		10 %

2 HNCA

Instrument

Model Bruker DMX (500 MHz)

Sample #1

Pressure 1 atm, Temperature 293 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	dematin	[U-13C; U-15N]	1 mM
2	phosphat		10 mM
3	TMSP		0.5 mM
4	sodium azide		0.01 %
5	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 293 K, pH 6.0

#	Name	Isotope labeling	Concentration
6	dematin	[U-15N]	4 mM
7	phosphat		10 mM
8	TMSP		0.5 mM
9	sodium azide		0.01 %
10	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 293 K, pH 6.0

#	Name	Isotope labeling	Concentration
11	dematin	[U-10% 13C; U-15N]	0.7 mM
12	phosphat		10 mM
13	TMSP		0.5 mM
14	sodium azide		0.01 %
15	D2O		10 %

3 HNCOCA

Instrument

Model Bruker DMX (500 MHz)

Sample #1

Pressure 1 atm, Temperature 293 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	dematin	[U-13C; U-15N]	1 mM
2	phosphat		10 mM
3	TMSP		0.5 mM
4	sodium azide		0.01 %
5	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 293 K, pH 6.0

#	Name	Isotope labeling	Concentration
6	dematin	[U-15N]	4 mM
7	phosphat		10 mM
8	TMSP		0.5 mM
9	sodium azide		0.01 %
10	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 293 K, pH 6.0

#	Name	Isotope labeling	Concentration
11	dematin	[U-10% 13C; U-15N]	0.7 mM
12	phosphat		10 mM
13	TMSP		0.5 mM
14	sodium azide		0.01 %
15	D2O		10 %

4 HNCO

Instrument

Model Bruker DMX (500 MHz)

Sample #1

Pressure 1 atm, Temperature 293 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	dematin	[U-13C; U-15N]	1 mM
2	phosphat		10 mM
3	TMSP		0.5 mM
4	sodium azide		0.01 %
5	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 293 K, pH 6.0

#	Name	Isotope labeling	Concentration
6	dematin	[U-15N]	4 mM
7	phosphat		10 mM
8	TMSP		0.5 mM
9	sodium azide		0.01 %
10	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 293 K, pH 6.0

#	Name	Isotope labeling	Concentration
11	dematin	[U-10% 13C; U-15N]	0.7 mM
12	phosphat		10 mM
13	TMSP		0.5 mM
14	sodium azide		0.01 %
15	D2O		10 %

5 HNCACO

Instrument

Model Bruker DMX (500 MHz)

Sample #1

Pressure 1 atm, Temperature 293 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	dematin	[U-13C; U-15N]	1 mM
2	phosphat		10 mM
3	TMSP		0.5 mM
4	sodium azide		0.01 %
5	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 293 K, pH 6.0

#	Name	Isotope labeling	Concentration
6	dematin	[U-15N]	4 mM
7	phosphat		10 mM
8	TMSP		0.5 mM
9	sodium azide		0.01 %
10	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 293 K, pH 6.0

#	Name	Isotope labeling	Concentration
11	dematin	[U-10% 13C; U-15N]	0.7 mM
12	phosphat		10 mM
13	TMSP		0.5 mM
14	sodium azide		0.01 %
15	D2O		10 %

6 3D 15N-separated NOESY

Instrument

Model Bruker DMX (500 MHz)

Sample #1

Pressure 1 atm, Temperature 293 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	dematin	[U-13C; U-15N]	1 mM
2	phosphat		10 mM
3	TMSP		0.5 mM
4	sodium azide		0.01 %
5	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 293 K, pH 6.0

#	Name	Isotope labeling	Concentration
6	dematin	[U-15N]	4 mM
7	phosphat		10 mM
8	TMSP		0.5 mM
9	sodium azide		0.01 %
10	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 293 K, pH 6.0

#	Name	Isotope labeling	Concentration
11	dematin	[U-10% 13C; U-15N]	0.7 mM
12	phosphat		10 mM
13	TMSP		0.5 mM
14	sodium azide		0.01 %
15	D2O		10 %

7 3D 15N-separated TOCSY

Instrument

Model Bruker DMX (500 MHz)

Sample #1

Pressure 1 atm, Temperature 293 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	dematin	[U-13C; U-15N]	1 mM
2	phosphat		10 mM
3	TMSP		0.5 mM
4	sodium azide		0.01 %
5	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 293 K, pH 6.0

#	Name	Isotope labeling	Concentration
6	dematin	[U-15N]	4 mM
7	phosphat		10 mM
8	TMSP		0.5 mM
9	sodium azide		0.01 %
10	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 293 K, pH 6.0

#	Name	Isotope labeling	Concentration
11	dematin	[U-10% 13C; U-15N]	0.7 mM
12	phosphat		10 mM
13	TMSP		0.5 mM
14	sodium azide		0.01 %
15	D2O		10 %

8 HCCH-COSY

Instrument

Model Bruker DMX (500 MHz)

Sample #1

Pressure 1 atm, Temperature 293 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	dematin	[U-13C; U-15N]	1 mM
2	phosphat		10 mM
3	TMSP		0.5 mM
4	sodium azide		0.01 %
5	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 293 K, pH 6.0

#	Name	Isotope labeling	Concentration
6	dematin	[U-15N]	4 mM
7	phosphat		10 mM
8	TMSP		0.5 mM
9	sodium azide		0.01 %
10	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 293 K, pH 6.0

#	Name	Isotope labeling	Concentration
11	dematin	[U-10% 13C; U-15N]	0.7 mM
12	phosphat		10 mM
13	TMSP		0.5 mM
14	sodium azide		0.01 %
15	D2O		10 %

9 2D NOESY

Instrument

Model Bruker DMX (500 MHz)

Sample #1

Pressure 1 atm, Temperature 293 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	dematin	[U-13C; U-15N]	1 mM
2	phosphat		10 mM
3	TMSP		0.5 mM
4	sodium azide		0.01 %
5	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 293 K, pH 6.0

#	Name	Isotope labeling	Concentration
6	dematin	[U-15N]	4 mM
7	phosphat		10 mM
8	TMSP		0.5 mM
9	sodium azide		0.01 %
10	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 293 K, pH 6.0

#	Name	Isotope labeling	Concentration
11	dematin	[U-10% 13C; U-15N]	0.7 mM
12	phosphat		10 mM
13	TMSP		0.5 mM
14	sodium azide		0.01 %
15	D2O		10 %

10 2D TOCSY

Instrument

Model Bruker DMX (500 MHz)

Sample #1

Pressure 1 atm, Temperature 293 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	dematin	[U-13C; U-15N]	1 mM
2	phosphat		10 mM
3	TMSP		0.5 mM
4	sodium azide		0.01 %
5	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 293 K, pH 6.0

#	Name	Isotope labeling	Concentration
6	dematin	[U-15N]	4 mM
7	phosphat		10 mM
8	TMSP		0.5 mM
9	sodium azide		0.01 %
10	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 293 K, pH 6.0

#	Name	Isotope labeling	Concentration
11	dematin	[U-10% 13C; U-15N]	0.7 mM
12	phosphat		10 mM
13	TMSP		0.5 mM
14	sodium azide		0.01 %
15	D2O		10 %

nullKeywords DEMATIN HEADPIECE, VILLIN HEADPIECE, ACTIN BINDING DOMAIN

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BMRB: 5968

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. dematin, : 1 : 1 : 107 entities Detail

Interaction partners 1. dematin, : 7 interactors Detail

Experiments performed 10 experiments Detail

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