BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	61.0 % (864 of 1416)	65.5 % (485 of 741)	48.0 % (264 of 550)	92.0 % (115 of 125)
Backbone	78.3 % (562 of 718)	89.9 % (222 of 247)	65.3 % (230 of 352)	92.4 % (110 of 119)
Sidechain	50.2 % (407 of 810)	53.2 % (263 of 494)	44.8 % (139 of 310)	83.3 % (5 of 6)
Aromatic	17.4 % (15 of 86)	34.9 % (15 of 43)	0.0 % (0 of 43)
Methyl	63.7 % (93 of 146)	78.1 % (57 of 73)	49.3 % (36 of 73)

1. Bacillus stearothermophius L18

MITKVDRNAV RKKRHARIRK KIFGTTERPR LSVFRSNKHI YAQIIDDTKS ATIVSASTLD KEFGLDSTNN IEAAKKVGEL VAKRALEKGI KQVVFDRGGY LYHGRVKALA DAAREAGLEF

Show sample condition

Sample

Pressure 1 atm, Temperature 298 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	Bacillus stearothermophius L18	[U-15N]	1.5 mM
2	phosphate buffer		20 mM
3	NaCl		200 mM
4	MgCl2		5 mM
5	H2O		90 %
6	D2O		10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	null	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 1.19 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	null	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 1.19 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	null	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.08 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 1OVY, Strand ID: A Detail

Release date

2004-02-12

Citation

The solution structure of ribosomal protein L18 from Bacillus stearothermophilus
Turner, C.F., Moore, P.B.
J. Mol. Biol. (2004), 335, 679-684, PubMed 14687565 , DOI: , Abstract

Related entities 1. 50S Ribosomal Protein L18, : 1 : 1 : 337 entities Detail

Experiments performed 10 experiments Detail

1 1H-15N HSQC

Instrument

Model Varian UNITY (600 MHz)

Sample #1

Pressure 1 atm, Temperature 298 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	Bacillus stearothermophius L18	[U-15N]	1.5 mM
2	phosphate buffer		20 mM
3	NaCl		200 mM
4	MgCl2		5 mM
5	H2O		90 %
6	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 298 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Concentration
7	Bacillus stearothermophius L18	[U-15N; U-13C]	1.5 mM
8	phosphate buffer		20 mM
9	NaCl		200 mM
10	MgCl2		5 mM
11	H2O		90 %
12	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 298 (±0.1) K, pH 6.0 (±0.1)

#	Name	Concentration
13	Bacillus stearothermophius L18	1 mM
14	phosphate buffer	20 mM
15	NaCl	200 mM
16	MgCl2	5 mM
17	H2O	90 %
18	D2O	10 %

2 HNCA

Instrument

Model Varian UNITY (600 MHz)

Sample #1

Pressure 1 atm, Temperature 298 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	Bacillus stearothermophius L18	[U-15N]	1.5 mM
2	phosphate buffer		20 mM
3	NaCl		200 mM
4	MgCl2		5 mM
5	H2O		90 %
6	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 298 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Concentration
7	Bacillus stearothermophius L18	[U-15N; U-13C]	1.5 mM
8	phosphate buffer		20 mM
9	NaCl		200 mM
10	MgCl2		5 mM
11	H2O		90 %
12	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 298 (±0.1) K, pH 6.0 (±0.1)

#	Name	Concentration
13	Bacillus stearothermophius L18	1 mM
14	phosphate buffer	20 mM
15	NaCl	200 mM
16	MgCl2	5 mM
17	H2O	90 %
18	D2O	10 %

3 HNCACB

Instrument

Model Varian UNITY (600 MHz)

Sample #1

Pressure 1 atm, Temperature 298 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	Bacillus stearothermophius L18	[U-15N]	1.5 mM
2	phosphate buffer		20 mM
3	NaCl		200 mM
4	MgCl2		5 mM
5	H2O		90 %
6	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 298 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Concentration
7	Bacillus stearothermophius L18	[U-15N; U-13C]	1.5 mM
8	phosphate buffer		20 mM
9	NaCl		200 mM
10	MgCl2		5 mM
11	H2O		90 %
12	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 298 (±0.1) K, pH 6.0 (±0.1)

#	Name	Concentration
13	Bacillus stearothermophius L18	1 mM
14	phosphate buffer	20 mM
15	NaCl	200 mM
16	MgCl2	5 mM
17	H2O	90 %
18	D2O	10 %

4 HNCO

Instrument

Model Varian UNITY (600 MHz)

Sample #1

Pressure 1 atm, Temperature 298 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	Bacillus stearothermophius L18	[U-15N]	1.5 mM
2	phosphate buffer		20 mM
3	NaCl		200 mM
4	MgCl2		5 mM
5	H2O		90 %
6	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 298 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Concentration
7	Bacillus stearothermophius L18	[U-15N; U-13C]	1.5 mM
8	phosphate buffer		20 mM
9	NaCl		200 mM
10	MgCl2		5 mM
11	H2O		90 %
12	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 298 (±0.1) K, pH 6.0 (±0.1)

#	Name	Concentration
13	Bacillus stearothermophius L18	1 mM
14	phosphate buffer	20 mM
15	NaCl	200 mM
16	MgCl2	5 mM
17	H2O	90 %
18	D2O	10 %

5 HNCACO

Instrument

Model Varian UNITY (600 MHz)

Sample #1

Pressure 1 atm, Temperature 298 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	Bacillus stearothermophius L18	[U-15N]	1.5 mM
2	phosphate buffer		20 mM
3	NaCl		200 mM
4	MgCl2		5 mM
5	H2O		90 %
6	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 298 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Concentration
7	Bacillus stearothermophius L18	[U-15N; U-13C]	1.5 mM
8	phosphate buffer		20 mM
9	NaCl		200 mM
10	MgCl2		5 mM
11	H2O		90 %
12	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 298 (±0.1) K, pH 6.0 (±0.1)

#	Name	Concentration
13	Bacillus stearothermophius L18	1 mM
14	phosphate buffer	20 mM
15	NaCl	200 mM
16	MgCl2	5 mM
17	H2O	90 %
18	D2O	10 %

6 HCCH-TOCSY

Instrument

Model Varian UNITY (600 MHz)

Sample #1

Pressure 1 atm, Temperature 298 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	Bacillus stearothermophius L18	[U-15N]	1.5 mM
2	phosphate buffer		20 mM
3	NaCl		200 mM
4	MgCl2		5 mM
5	H2O		90 %
6	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 298 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Concentration
7	Bacillus stearothermophius L18	[U-15N; U-13C]	1.5 mM
8	phosphate buffer		20 mM
9	NaCl		200 mM
10	MgCl2		5 mM
11	H2O		90 %
12	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 298 (±0.1) K, pH 6.0 (±0.1)

#	Name	Concentration
13	Bacillus stearothermophius L18	1 mM
14	phosphate buffer	20 mM
15	NaCl	200 mM
16	MgCl2	5 mM
17	H2O	90 %
18	D2O	10 %

7 15N TOCSY-HSQC

Instrument

Model Varian UNITY (600 MHz)

Sample #1

Pressure 1 atm, Temperature 298 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	Bacillus stearothermophius L18	[U-15N]	1.5 mM
2	phosphate buffer		20 mM
3	NaCl		200 mM
4	MgCl2		5 mM
5	H2O		90 %
6	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 298 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Concentration
7	Bacillus stearothermophius L18	[U-15N; U-13C]	1.5 mM
8	phosphate buffer		20 mM
9	NaCl		200 mM
10	MgCl2		5 mM
11	H2O		90 %
12	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 298 (±0.1) K, pH 6.0 (±0.1)

#	Name	Concentration
13	Bacillus stearothermophius L18	1 mM
14	phosphate buffer	20 mM
15	NaCl	200 mM
16	MgCl2	5 mM
17	H2O	90 %
18	D2O	10 %

8 15N NOESY-HSQC

Instrument

Model Varian UNITY (600 MHz)

Sample #1

Pressure 1 atm, Temperature 298 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	Bacillus stearothermophius L18	[U-15N]	1.5 mM
2	phosphate buffer		20 mM
3	NaCl		200 mM
4	MgCl2		5 mM
5	H2O		90 %
6	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 298 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Concentration
7	Bacillus stearothermophius L18	[U-15N; U-13C]	1.5 mM
8	phosphate buffer		20 mM
9	NaCl		200 mM
10	MgCl2		5 mM
11	H2O		90 %
12	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 298 (±0.1) K, pH 6.0 (±0.1)

#	Name	Concentration
13	Bacillus stearothermophius L18	1 mM
14	phosphate buffer	20 mM
15	NaCl	200 mM
16	MgCl2	5 mM
17	H2O	90 %
18	D2O	10 %

9 2D NOESY

Instrument

Model Varian UNITY (600 MHz)

Sample #1

Pressure 1 atm, Temperature 298 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	Bacillus stearothermophius L18	[U-15N]	1.5 mM
2	phosphate buffer		20 mM
3	NaCl		200 mM
4	MgCl2		5 mM
5	H2O		90 %
6	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 298 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Concentration
7	Bacillus stearothermophius L18	[U-15N; U-13C]	1.5 mM
8	phosphate buffer		20 mM
9	NaCl		200 mM
10	MgCl2		5 mM
11	H2O		90 %
12	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 298 (±0.1) K, pH 6.0 (±0.1)

#	Name	Concentration
13	Bacillus stearothermophius L18	1 mM
14	phosphate buffer	20 mM
15	NaCl	200 mM
16	MgCl2	5 mM
17	H2O	90 %
18	D2O	10 %

10 13C NOESY-HSQC

Instrument

Model Varian UNITY (600 MHz)

Sample #1

Pressure 1 atm, Temperature 298 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	Bacillus stearothermophius L18	[U-15N]	1.5 mM
2	phosphate buffer		20 mM
3	NaCl		200 mM
4	MgCl2		5 mM
5	H2O		90 %
6	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 298 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Concentration
7	Bacillus stearothermophius L18	[U-15N; U-13C]	1.5 mM
8	phosphate buffer		20 mM
9	NaCl		200 mM
10	MgCl2		5 mM
11	H2O		90 %
12	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 298 (±0.1) K, pH 6.0 (±0.1)

#	Name	Concentration
13	Bacillus stearothermophius L18	1 mM
14	phosphate buffer	20 mM
15	NaCl	200 mM
16	MgCl2	5 mM
17	H2O	90 %
18	D2O	10 %

nullKeywords Ribosomal Protein

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Found 1 Document (6.958 seconds)

BMRB: 5970

Sample

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Chemical shift reference

Chemical shift reference

Related entities 1. 50S Ribosomal Protein L18, : 1 : 1 : 337 entities Detail

Experiments performed 10 experiments Detail

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