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Found 1 Document (1.622 seconds)

BMRB: 5971

1Z66

Letter to the Editor: Backbone and side chain resonance assignments of domain III of tick-borne Langat flavivirus Envelope protein

Authors

Mukherjee, M., Dutta, K., Pascal, S.M., Fox, R.O.

Assembly

Domain III of the E protein of Langat flavivirus

Entity

1. Domain III of the E protein of Langat flavivirus (polymer, Thiol state: all disulfide bound), 108 monomers, 11722.17 Da Detail

GSEFGSKGLT YTVCDKTKFT WKRAPTDSGH DTVVMEVGFS GTRPCRIPVR AVAHGVPEVN VAMLITPNPT MENNGGGFIE MQLPPGDNII YVGDLNHQWF QKGSSIGR

Formula weight

11722.17 Da

Entity Connection

disulfide 1 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS14:SG	1:CYS45:SG

Source organism

Langat virus

Exptl. method

NMR

Refine. method

distance geometry and simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.1 %, Completeness: 84.7 %, Completeness (bb): 96.5 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	84.7 % (1030 of 1216)	81.3 % (512 of 630)	86.1 % (409 of 475)	98.2 % (109 of 111)
Backbone	96.5 % (610 of 632)	95.9 % (213 of 222)	96.5 % (299 of 310)	98.0 % (98 of 100)
Sidechain	75.7 % (513 of 678)	73.3 % (299 of 408)	78.4 % (203 of 259)	100.0 % (11 of 11)
Aromatic	27.5 % (28 of 102)	27.5 % (14 of 51)	24.5 % (12 of 49)	100.0 % (2 of 2)
Methyl	84.0 % (89 of 106)	84.9 % (45 of 53)	83.0 % (44 of 53)

1. Langat E-D3

GSEFGSKGLT YTVCDKTKFT WKRAPTDSGH DTVVMEVGFS GTRPCRIPVR AVAHGVPEVN VAMLITPNPT MENNGGGFIE MQLPPGDNII YVGDLNHQWF QKGSSIGR

Show sample condition

Sample

Temperature 298 (±0.1) K, pH 6.1 (±0.1)

#	Name	Isotope labeling	Concentration
1	Langat E-D3	[U-100% 13C; U-100% 15N]	0.7 mM
2	Tris		10 mM
3	Bis-Tris		50 mM
4	NaCL		10 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.14 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.14 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.09 ppm, Outliers: 4 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.41 ppm, Outliers: 3 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 1Z66, Strand ID: A Detail

Release date

2004-07-27

Citation

Backbone and side chain resonance assignments of domain III of the tick-borne Langat flavivirus envelope protein
Mukherjee, M., Dutta, K., Pascal, S.M., Fox, R.O.
J. Biomol. NMR (2004), 29, 535-536, PubMed 15243186 , DOI 10.1023/B:JNMR.0000034345.94232.16 , Abstract

Related entities 1. Domain III of the E protein of Langat flavivirus, : 1 : 3 : 174 entities Detail

Experiments performed 12 experiments Detail

1 15N-HSQC

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Temperature 298 (±0.1) K, pH 6.1 (±0.1)

#	Name	Isotope labeling	Concentration
1	Langat E-D3	[U-100% 13C; U-100% 15N]	0.7 mM
2	Tris		10 mM
3	Bis-Tris		50 mM
4	NaCL		10 mM

Sample #2

Temperature 298 (±0.1) K, pH 6.1 (±0.1)

#	Name	Isotope labeling	Type	Concentration
5	Langat E-D3	[U-99% 15N]		0.8 mM

2 13C-HSQC

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Temperature 298 (±0.1) K, pH 6.1 (±0.1)

#	Name	Isotope labeling	Concentration
1	Langat E-D3	[U-100% 13C; U-100% 15N]	0.7 mM
2	Tris		10 mM
3	Bis-Tris		50 mM
4	NaCL		10 mM

Sample #2

Temperature 298 (±0.1) K, pH 6.1 (±0.1)

#	Name	Isotope labeling	Type	Concentration
5	Langat E-D3	[U-99% 15N]		0.8 mM

3 HNCO

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Temperature 298 (±0.1) K, pH 6.1 (±0.1)

#	Name	Isotope labeling	Concentration
1	Langat E-D3	[U-100% 13C; U-100% 15N]	0.7 mM
2	Tris		10 mM
3	Bis-Tris		50 mM
4	NaCL		10 mM

Sample #2

Temperature 298 (±0.1) K, pH 6.1 (±0.1)

#	Name	Isotope labeling	Type	Concentration
5	Langat E-D3	[U-99% 15N]		0.8 mM

4 CBCACONH

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Temperature 298 (±0.1) K, pH 6.1 (±0.1)

#	Name	Isotope labeling	Concentration
1	Langat E-D3	[U-100% 13C; U-100% 15N]	0.7 mM
2	Tris		10 mM
3	Bis-Tris		50 mM
4	NaCL		10 mM

Sample #2

Temperature 298 (±0.1) K, pH 6.1 (±0.1)

#	Name	Isotope labeling	Type	Concentration
5	Langat E-D3	[U-99% 15N]		0.8 mM

5 HNCACB

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Temperature 298 (±0.1) K, pH 6.1 (±0.1)

#	Name	Isotope labeling	Concentration
1	Langat E-D3	[U-100% 13C; U-100% 15N]	0.7 mM
2	Tris		10 mM
3	Bis-Tris		50 mM
4	NaCL		10 mM

Sample #2

Temperature 298 (±0.1) K, pH 6.1 (±0.1)

#	Name	Isotope labeling	Type	Concentration
5	Langat E-D3	[U-99% 15N]		0.8 mM

6 C(C0)NH

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Temperature 298 (±0.1) K, pH 6.1 (±0.1)

#	Name	Isotope labeling	Concentration
1	Langat E-D3	[U-100% 13C; U-100% 15N]	0.7 mM
2	Tris		10 mM
3	Bis-Tris		50 mM
4	NaCL		10 mM

Sample #2

Temperature 298 (±0.1) K, pH 6.1 (±0.1)

#	Name	Isotope labeling	Type	Concentration
5	Langat E-D3	[U-99% 15N]		0.8 mM

7 HBHACONH

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Temperature 298 (±0.1) K, pH 6.1 (±0.1)

#	Name	Isotope labeling	Concentration
1	Langat E-D3	[U-100% 13C; U-100% 15N]	0.7 mM
2	Tris		10 mM
3	Bis-Tris		50 mM
4	NaCL		10 mM

Sample #2

Temperature 298 (±0.1) K, pH 6.1 (±0.1)

#	Name	Isotope labeling	Type	Concentration
5	Langat E-D3	[U-99% 15N]		0.8 mM

8 HC(CO)NH

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Temperature 298 (±0.1) K, pH 6.1 (±0.1)

#	Name	Isotope labeling	Concentration
1	Langat E-D3	[U-100% 13C; U-100% 15N]	0.7 mM
2	Tris		10 mM
3	Bis-Tris		50 mM
4	NaCL		10 mM

Sample #2

Temperature 298 (±0.1) K, pH 6.1 (±0.1)

#	Name	Isotope labeling	Type	Concentration
5	Langat E-D3	[U-99% 15N]		0.8 mM

9 HCCH-TOCSY

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Temperature 298 (±0.1) K, pH 6.1 (±0.1)

#	Name	Isotope labeling	Concentration
1	Langat E-D3	[U-100% 13C; U-100% 15N]	0.7 mM
2	Tris		10 mM
3	Bis-Tris		50 mM
4	NaCL		10 mM

Sample #2

Temperature 298 (±0.1) K, pH 6.1 (±0.1)

#	Name	Isotope labeling	Type	Concentration
5	Langat E-D3	[U-99% 15N]		0.8 mM

10 N15-NOESY-HSQC

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Temperature 298 (±0.1) K, pH 6.1 (±0.1)

#	Name	Isotope labeling	Concentration
1	Langat E-D3	[U-100% 13C; U-100% 15N]	0.7 mM
2	Tris		10 mM
3	Bis-Tris		50 mM
4	NaCL		10 mM

Sample #2

Temperature 298 (±0.1) K, pH 6.1 (±0.1)

#	Name	Isotope labeling	Type	Concentration
5	Langat E-D3	[U-99% 15N]		0.8 mM

11 N15-TOCSY-HSQC

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Temperature 298 (±0.1) K, pH 6.1 (±0.1)

#	Name	Isotope labeling	Concentration
1	Langat E-D3	[U-100% 13C; U-100% 15N]	0.7 mM
2	Tris		10 mM
3	Bis-Tris		50 mM
4	NaCL		10 mM

Sample #2

Temperature 298 (±0.1) K, pH 6.1 (±0.1)

#	Name	Isotope labeling	Type	Concentration
5	Langat E-D3	[U-99% 15N]		0.8 mM

12 C13-NOESY-HSQC

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Temperature 298 (±0.1) K, pH 6.1 (±0.1)

#	Name	Isotope labeling	Concentration
1	Langat E-D3	[U-100% 13C; U-100% 15N]	0.7 mM
2	Tris		10 mM
3	Bis-Tris		50 mM
4	NaCL		10 mM

Sample #2

Temperature 298 (±0.1) K, pH 6.1 (±0.1)

#	Name	Isotope labeling	Type	Concentration
5	Langat E-D3	[U-99% 15N]		0.8 mM

nullKeywords Flavivirus, Langat, Domain III, NMR

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Found 1 Document (1.622 seconds)

BMRB: 5971

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. Domain III of the E protein of Langat flavivirus, : 1 : 3 : 174 entities Detail

Experiments performed 12 experiments Detail

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