BMRBj - BMREntryMaster

Found 1 Document (2.966 seconds)

BMRB: 5975

1R2U

1H and 15N chemical shift assignments for the N-domain of trout cardiac troponin C at 30 and 7 C

Authors

Blumenschein, T.M. A., Gillis, T.E., Tibbits, G.F., Sykes, B.D.

Assembly

N-terminal domain of trout cardiac troponin C

Entity

1. trout troponin C (polymer, Thiol state: all free), 89 monomers, 10134.26 Da Detail

MNDIYKAAVE QLTDEQKNEF KAAFDIFIQD AEDGCISTKE LGKVMRMLGQ NPTPEELQEM IDEVDEDGSG TVDFDEFLVM MVRCMKDDS

2. CA (non-polymer), 40.078 Da

Total weight

10174.338 Da

Max. entity weight

10134.26 Da

Source organism

Oncorhynchus mykiss

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete1

Sequence coverage: 98.9 %, Completeness: 74.2 %, Completeness (bb): 74.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	74.2 % (757 of 1020)	85.9 % (457 of 532)	53.4 % (210 of 393)	94.7 % (90 of 95)
Backbone	74.3 % (394 of 530)	95.0 % (172 of 181)	53.4 % (140 of 262)	94.3 % (82 of 87)
Sidechain	73.9 % (424 of 574)	81.2 % (285 of 351)	60.9 % (131 of 215)	100.0 % (8 of 8)
Aromatic	24.1 % (14 of 58)	48.3 % (14 of 29)	0.0 % (0 of 29)
Methyl	85.4 % (70 of 82)	95.1 % (39 of 41)	75.6 % (31 of 41)

1. trout troponin C

MNDIYKAAVE QLTDEQKNEF KAAFDIFIQD AEDGCISTKE LGKVMRMLGQ NPTPEELQEM IDEVDEDGSG TVDFDEFLVM MVRCMKDDS

Show sample condition

Sample #1

Temperature 303 (±1) K, pH 6.9 (±0.1)

#	Name	Isotope labeling	Concentration
1	trout troponin C	[U-90% 15N]	1.34 mM
2	imidazole		10 mM
3	KCl		100 mM
4	dithiothreitol		20 mM
5	NaN3		0.03 %
6	2,2-dimethyl-2-silapentane-5-sulfonic_acid_(DSS)		0.2 mM

Sample #2

Temperature 303 (±1) K, pH 6.9 (±0.1)

#	Name	Isotope labeling	Concentration
7	trout troponin C	[U-96% 15N]	1.34 mM
8	imidazole		10 mM
9	KCl		100 mM
10	dithiothreitol		20 mM
11	NaN3		0.03 %
12	2,2-dimethyl-2-silapentane-5-sulfonic_acid_(DSS)		0.2 mM

Chem. Shift Complete2

Sequence coverage: 100.0 %, Completeness: 72.8 %, Completeness (bb): 70.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	72.8 % (1485 of 2040)	87.5 % (931 of 1064)	47.3 % (372 of 786)	95.8 % (182 of 190)
Backbone	70.1 % (743 of 1060)	96.7 % (350 of 362)	43.3 % (227 of 524)	95.4 % (166 of 174)
Sidechain	73.4 % (843 of 1148)	82.8 % (581 of 702)	57.2 % (246 of 430)	100.0 % (16 of 16)
Aromatic	27.6 % (32 of 116)	50.0 % (29 of 58)	5.2 % (3 of 58)
Methyl	88.4 % (145 of 164)	96.3 % (79 of 82)	80.5 % (66 of 82)

1. trout troponin C

MNDIYKAAVE QLTDEQKNEF KAAFDIFIQD AEDGCISTKE LGKVMRMLGQ NPTPEELQEM IDEVDEDGSG TVDFDEFLVM MVRCMKDDS

Show sample condition

Sample

Temperature 280 (±1) K, pH 6.9 (±0.1)

#	Name	Isotope labeling	Concentration
7	trout troponin C	[U-96% 15N]	1.34 mM
8	imidazole		10 mM
9	KCl		100 mM
10	dithiothreitol		20 mM
11	NaN3		0.03 %
12	2,2-dimethyl-2-silapentane-5-sulfonic_acid_(DSS)		0.2 mM

Protein Blocks Logo

Calculated from 40 models in PDB: 1R2U, Strand ID: A Detail

Release date

2003-12-07

Citation

Effect of temperature and the F27W mutation on the Ca2+ activated structural transition of trout cardiac troponin C
Gillis, T.E., Blumenschein, T.M. A., Sykes, B.D., Tibbits, G.F.
Biochemistry (2003), 42, 6418-6426, PubMed 12767223 , DOI 10.1021/bi0340494 , Abstract

Related entities 1. trout troponin C, : 1 : 1 : 1 : 297 entities Detail

Interaction partners 1. trout troponin C, : 8 interactors Detail

More details for 8 interactors identified by 5 citations

Experiments performed 6 experiments Detail

1 1H-15N HSQC

Instrument

#1 Model Varian INOVA (500 MHz)

#2 Model Varian Unity (600 MHz)

#3 Model Varian INOVA (800 MHz)

Sample #1

pH 6.9 (±0.1)

#	Name	Isotope labeling	Concentration
1	trout troponin C	[U-90% 15N]	1.34 mM
2	imidazole		10 mM
3	KCl		100 mM
4	dithiothreitol		20 mM
5	NaN3		0.03 %
6	2,2-dimethyl-2-silapentane-5-sulfonic_acid_(DSS)		0.2 mM

Sample #2

pH 6.9 (±0.1)

#	Name	Isotope labeling	Concentration
7	trout troponin C	[U-96% 15N]	1.34 mM
8	imidazole		10 mM
9	KCl		100 mM
10	dithiothreitol		20 mM
11	NaN3		0.03 %
12	2,2-dimethyl-2-silapentane-5-sulfonic_acid_(DSS)		0.2 mM

2 15N DIPSI-HSQC

Instrument

#1 Model Varian INOVA (500 MHz)

#2 Model Varian Unity (600 MHz)

#3 Model Varian INOVA (800 MHz)

Sample #1

pH 6.9 (±0.1)

#	Name	Isotope labeling	Concentration
1	trout troponin C	[U-90% 15N]	1.34 mM
2	imidazole		10 mM
3	KCl		100 mM
4	dithiothreitol		20 mM
5	NaN3		0.03 %
6	2,2-dimethyl-2-silapentane-5-sulfonic_acid_(DSS)		0.2 mM

Sample #2

pH 6.9 (±0.1)

#	Name	Isotope labeling	Concentration
7	trout troponin C	[U-96% 15N]	1.34 mM
8	imidazole		10 mM
9	KCl		100 mM
10	dithiothreitol		20 mM
11	NaN3		0.03 %
12	2,2-dimethyl-2-silapentane-5-sulfonic_acid_(DSS)		0.2 mM

3 15N NOESY-HSQC

Instrument

#1 Model Varian INOVA (500 MHz)

#2 Model Varian Unity (600 MHz)

#3 Model Varian INOVA (800 MHz)

Sample #1

pH 6.9 (±0.1)

#	Name	Isotope labeling	Concentration
1	trout troponin C	[U-90% 15N]	1.34 mM
2	imidazole		10 mM
3	KCl		100 mM
4	dithiothreitol		20 mM
5	NaN3		0.03 %
6	2,2-dimethyl-2-silapentane-5-sulfonic_acid_(DSS)		0.2 mM

Sample #2

pH 6.9 (±0.1)

#	Name	Isotope labeling	Concentration
7	trout troponin C	[U-96% 15N]	1.34 mM
8	imidazole		10 mM
9	KCl		100 mM
10	dithiothreitol		20 mM
11	NaN3		0.03 %
12	2,2-dimethyl-2-silapentane-5-sulfonic_acid_(DSS)		0.2 mM

4 HNHA

Instrument

#1 Model Varian INOVA (500 MHz)

#2 Model Varian Unity (600 MHz)

#3 Model Varian INOVA (800 MHz)

Sample #1

pH 6.9 (±0.1)

#	Name	Isotope labeling	Concentration
1	trout troponin C	[U-90% 15N]	1.34 mM
2	imidazole		10 mM
3	KCl		100 mM
4	dithiothreitol		20 mM
5	NaN3		0.03 %
6	2,2-dimethyl-2-silapentane-5-sulfonic_acid_(DSS)		0.2 mM

Sample #2

pH 6.9 (±0.1)

#	Name	Isotope labeling	Concentration
7	trout troponin C	[U-96% 15N]	1.34 mM
8	imidazole		10 mM
9	KCl		100 mM
10	dithiothreitol		20 mM
11	NaN3		0.03 %
12	2,2-dimethyl-2-silapentane-5-sulfonic_acid_(DSS)		0.2 mM

5 HNHB

Instrument

#1 Model Varian INOVA (500 MHz)

#2 Model Varian Unity (600 MHz)

#3 Model Varian INOVA (800 MHz)

Sample #1

pH 6.9 (±0.1)

#	Name	Isotope labeling	Concentration
1	trout troponin C	[U-90% 15N]	1.34 mM
2	imidazole		10 mM
3	KCl		100 mM
4	dithiothreitol		20 mM
5	NaN3		0.03 %
6	2,2-dimethyl-2-silapentane-5-sulfonic_acid_(DSS)		0.2 mM

Sample #2

pH 6.9 (±0.1)

#	Name	Isotope labeling	Concentration
7	trout troponin C	[U-96% 15N]	1.34 mM
8	imidazole		10 mM
9	KCl		100 mM
10	dithiothreitol		20 mM
11	NaN3		0.03 %
12	2,2-dimethyl-2-silapentane-5-sulfonic_acid_(DSS)		0.2 mM

6 2D-NOESY

Instrument

#1 Model Varian INOVA (500 MHz)

#2 Model Varian Unity (600 MHz)

#3 Model Varian INOVA (800 MHz)

Sample #1

pH 6.9 (±0.1)

#	Name	Isotope labeling	Concentration
1	trout troponin C	[U-90% 15N]	1.34 mM
2	imidazole		10 mM
3	KCl		100 mM
4	dithiothreitol		20 mM
5	NaN3		0.03 %
6	2,2-dimethyl-2-silapentane-5-sulfonic_acid_(DSS)		0.2 mM

Sample #2

pH 6.9 (±0.1)

#	Name	Isotope labeling	Concentration
7	trout troponin C	[U-96% 15N]	1.34 mM
8	imidazole		10 mM
9	KCl		100 mM
10	dithiothreitol		20 mM
11	NaN3		0.03 %
12	2,2-dimethyl-2-silapentane-5-sulfonic_acid_(DSS)		0.2 mM

nullKeywords EF-hand, troponin

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Found 1 Document (2.966 seconds)

BMRB: 5975

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Related entities 1. trout troponin C, : 1 : 1 : 1 : 297 entities Detail

Interaction partners 1. trout troponin C, : 8 interactors Detail

Experiments performed 6 experiments Detail

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