BMRBj - BMREntryMaster

Found 1 Document (1.36 seconds)

BMRB: 5979

1R2L

A parallel stranded DNA duplex with an A-G mismatch base-pair

Authors

Venkitakrishnan, R.P., Bhaumik, S.R., Chary, K.V.R., Govil, G., Liu, K., Howard, F.B., Miles, T.H.

Assembly

DNA

Entity

1. A-G mismatch base-pair parallel DNA duplex, strand A (polymer, Thiol state: not present), 12 monomers, 3644.340 Da Detail

CCATAATTTA CC

2. A-G mismatch base-pair parallel DNA duplex, strand B (polymer, Thiol state: not present), 12 monomers, 3669.353 Da Detail

CCTATGAAAT CC

Total weight

7313.6934 Da

Max. entity weight

3669.353 Da

Exptl. method

Theoretical

Data set

assigned_chemical_shifts

Chem. Shift Complete1

Sequence coverage: 50.0 %, Completeness: 45.2 %, Completeness (bb): 50.0 % Detail

Polymer type: polydeoxyribonucleotide

	Total	¹H
All	45.2 % (108 of 239)	45.2 % (108 of 239)
Suger, PO4	50.0 % (84 of 168)	50.0 % (84 of 168)
Nucleobase	33.8 % (24 of 71)	33.8 % (24 of 71)
Aromatic	41.7 % (20 of 48)	41.7 % (20 of 48)
Methyl	57.1 % (4 of 7)	57.1 % (4 of 7)

1. A-G mismatch base-pair parallel DNA duplex, strand A

CCATAATTTA CC

2. A-G mismatch base-pair parallel DNA duplex, strand B

CCTATGAAAT CC

Show sample condition

Sample

Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	A-G mismatch base-pair parallel DNA duplex, strand A		5 mM
2	A-G mismatch base-pair parallel DNA duplex, strand B		5 mM
3	sodium acetate buffer	[U-2H]	0.05 M
4	NaCl		0.1 M
5	D2O		100 %

Chem. Shift Complete2

Sequence coverage: 50.0 %, Completeness: 45.0 %, Completeness (bb): 50.0 % Detail

Polymer type: polydeoxyribonucleotide

	Total	¹H
All	45.0 % (215 of 478)	45.0 % (215 of 478)
Suger, PO4	50.0 % (168 of 336)	50.0 % (168 of 336)
Nucleobase	33.1 % (47 of 142)	33.1 % (47 of 142)
Aromatic	41.7 % (40 of 96)	41.7 % (40 of 96)
Methyl	50.0 % (7 of 14)	50.0 % (7 of 14)

1. A-G mismatch base-pair parallel DNA duplex, strand A

CCATAATTTA CC

2. A-G mismatch base-pair parallel DNA duplex, strand B

CCTATGAAAT CC

Show sample condition

Sample

Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	A-G mismatch base-pair parallel DNA duplex, strand A		5 mM
2	A-G mismatch base-pair parallel DNA duplex, strand B		5 mM
3	sodium acetate buffer	[U-2H]	0.05 M
4	NaCl		0.1 M
5	D2O		100 %

Release date

2003-01-19

Citation

A Parallel Stranded DNA Duplex with an A-G Mismatch Base-Pair: (Ccataatttacc:Cctatgaaatcc)
Venkitakrishnan, R.P., Bhaumik, S.R., Chary, K.V.R., Govil, G., Liu, K., Howard, F.B., Miles, T.H.
Recent Trends in Biophys. Res. (2004), 1

Experiments performed 4 experiments Detail

1 2D NOESY

Instrument

Model Bruker AMX (500 MHz)

Sample #1

Pressure 1 atm

#	Name	Isotope labeling	Concentration
1	A-G mismatch base-pair parallel DNA duplex, strand A		5 mM
2	A-G mismatch base-pair parallel DNA duplex, strand B		5 mM
3	sodium acetate buffer	[U-2H]	0.05 M
4	NaCl		0.1 M
5	D2O		100 %

Sample #2

Pressure 1 atm

#	Name	Isotope labeling	Concentration
6	A-G mismatch base-pair parallel DNA duplex, strand A		5 mM
7	A-G mismatch base-pair parallel DNA duplex, strand B		5 mM
8	sodium acetate buffer	[U-2H]	0.05 M
9	NaCl		0.1 M
10	D2O		10 %
11	H2O		90 %

2 2D TOCSY

Instrument

Model Bruker AMX (500 MHz)

Sample #1

Pressure 1 atm

#	Name	Isotope labeling	Concentration
1	A-G mismatch base-pair parallel DNA duplex, strand A		5 mM
2	A-G mismatch base-pair parallel DNA duplex, strand B		5 mM
3	sodium acetate buffer	[U-2H]	0.05 M
4	NaCl		0.1 M
5	D2O		100 %

Sample #2

Pressure 1 atm

#	Name	Isotope labeling	Concentration
6	A-G mismatch base-pair parallel DNA duplex, strand A		5 mM
7	A-G mismatch base-pair parallel DNA duplex, strand B		5 mM
8	sodium acetate buffer	[U-2H]	0.05 M
9	NaCl		0.1 M
10	D2O		10 %
11	H2O		90 %

3 jump return NOESY

Instrument

Model Bruker AMX (500 MHz)

Sample #1

Pressure 1 atm

#	Name	Isotope labeling	Concentration
1	A-G mismatch base-pair parallel DNA duplex, strand A		5 mM
2	A-G mismatch base-pair parallel DNA duplex, strand B		5 mM
3	sodium acetate buffer	[U-2H]	0.05 M
4	NaCl		0.1 M
5	D2O		100 %

Sample #2

Pressure 1 atm

#	Name	Isotope labeling	Concentration
6	A-G mismatch base-pair parallel DNA duplex, strand A		5 mM
7	A-G mismatch base-pair parallel DNA duplex, strand B		5 mM
8	sodium acetate buffer	[U-2H]	0.05 M
9	NaCl		0.1 M
10	D2O		10 %
11	H2O		90 %

4 E-COSY

Instrument

Model Bruker AMX (500 MHz)

Sample #1

Pressure 1 atm

#	Name	Isotope labeling	Concentration
1	A-G mismatch base-pair parallel DNA duplex, strand A		5 mM
2	A-G mismatch base-pair parallel DNA duplex, strand B		5 mM
3	sodium acetate buffer	[U-2H]	0.05 M
4	NaCl		0.1 M
5	D2O		100 %

Sample #2

Pressure 1 atm

#	Name	Isotope labeling	Concentration
6	A-G mismatch base-pair parallel DNA duplex, strand A		5 mM
7	A-G mismatch base-pair parallel DNA duplex, strand B		5 mM
8	sodium acetate buffer	[U-2H]	0.05 M
9	NaCl		0.1 M
10	D2O		10 %
11	H2O		90 %

nullKeywords A:G mismatch, parallel DNA duplex, ps-DNA

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Found 1 Document (1.36 seconds)

BMRB: 5979

Sample

Sample

Experiments performed 4 experiments Detail

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2