BMRB: 5985

Solution Conformation of alpha-Conotoxin GIC, a Novel Potent Antagonist of alpha3beta2 Nicotinic Acetylcholine Receptors

Authors

Chi, S., Kim, D., Olivera, B.M., McIntosh, J.M., Han, K.

Assembly

alpha-Conotoxin GIC

Entity

1. alpha-Conotoxin GIC (polymer, Thiol state: all disulfide bound), 17 monomers, 1612.815 Da Detail

GCCSHPACAG NNQHICX

Formula weight

1612.815 Da

Entity Connection

disulfide 2 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS2:SG	1:CYS8:SG
2	disulfide	sing	1:CYS3:SG	1:CYS16:SG

Source organism

Conus geographus

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts, coupling_constants

Chem. Shift Complete

Sequence coverage: 94.1 %, Completeness: 98.7 %, Completeness (bb): 97.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	98.7 % (77 of 78)	98.7 % (77 of 78)
Backbone	97.0 % (32 of 33)	97.0 % (32 of 33)
Sidechain	100.0 % (45 of 45)	100.0 % (45 of 45)
Aromatic	100.0 % (4 of 4)	100.0 % (4 of 4)
Methyl	100.0 % (4 of 4)	100.0 % (4 of 4)

1. alpha-conotoxin GIC

GCCSHPACAG NNQHICX

Show sample condition

Sample

Pressure 1 atm, Temperature 293 (±0.5) K, pH 3.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	alpha-conotoxin GIC			11.5 mM

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Protein Blocks Logo

Calculated from 20 models in PDB: 1UL2, Strand ID: A Detail

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Coupling constant

13 J values in 1 lists
Field strength (¹H) 600 MHz, Pressure 1 atm, Temperature 293 (±0.5) K, pH 3.5 (±0.1) Detail

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Pressure 1 atm, Temperature 293 (±0.5) K, pH 3.5 (±0.1)

Experiment name 2D NOESY

List #1 J_values_set_1, Field strength (¹H) 600 MHz

Release date

2003-10-27

Citation

Solution conformation of alpha-conotoxin GIC, a novel potent antagonist of alpha3beta2 nicotinic acetylcholine receptors
Chi, S., Kim, D., Olivera, B.M., McIntosh, J.M., Han, K.
Biochem. J. (2004), 380, 347-352, PubMed 14992691 , DOI 10.1042/BJ20031792 , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Related entities 1. alpha-Conotoxin GIC, : 1 : 2 : 43 entities Detail

Experiments performed 3 experiments Detail