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BMRB: 5991

1R36

Backbone and Sidechain 1H, 13C, and 15N Chemical Shift Assignments and Backbone 15N Relaxation Parameters for Murine Ets-1 deltaN301

Authors

Lee, G.M., Donaldson, L.W., Pufall, M.A., Kang, H., Gentile, L.N., Pot, I., Graves, B.J., McIntosh, L.P.

Assembly

Murine Ets-1 deltaN301

Entity

1. Murine Ets-1 deltaN301 (polymer, Thiol state: all free), 140 monomers, 16226.30 Da Detail

KGTFKDYVRD RADLNKDKPV IPAAALAGYT GSGPIQLWQF LLELLTDKSC QSFISWTGDG WEFKLSDPDE VARRWGKRKN KPKMNYEKLS RGLRYYYDKN IIHKTAGKRY VYRFVSDLQS LLGYTPEELH AMLDVKPDAD

Formula weight

16226.3 Da

Source organism

Mus musculus

Exptl. method

NMR

Refine. method

molecular dynamics

Data set

assigned_chemical_shifts, heteronucl_T1_relaxation, heteronucl_T2_relaxation, heteronucl_NOEs, order_parameters

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 97.7 %, Completeness (bb): 97.5 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	97.7 % (1663 of 1703)	98.0 % (876 of 894)	96.8 % (643 of 664)	99.3 % (144 of 145)
Backbone	97.5 % (805 of 826)	98.6 % (279 of 283)	96.1 % (394 of 410)	99.2 % (132 of 133)
Sidechain	98.1 % (988 of 1007)	97.9 % (598 of 611)	98.4 % (378 of 384)	100.0 % (12 of 12)
Aromatic	97.8 % (174 of 178)	97.8 % (87 of 89)	97.6 % (83 of 85)	100.0 % (4 of 4)
Methyl	97.0 % (130 of 134)	97.0 % (65 of 67)	97.0 % (65 of 67)

1. Ets-1

KGTFKDYVRD RADLNKDKPV IPAAALAGYT GSGPIQLWQF LLELLTDKSC QSFISWTGDG WEFKLSDPDE VARRWGKRKN KPKMNYEKLS RGLRYYYDKN IIHKTAGKRY VYRFVSDLQS LLGYTPEELH AMLDVKPDAD

Show sample condition

Sample #1

Temperature 301 (±1) K, pH 6.5 (±0.1), Details Samples irreversibly aggregated at NMR conditions after approximately 1 week.

#	Name	Isotope labeling	Concentration
1	Ets-1	[U-99% 15N; U-99% 13C]	0.3 ~ 0.8 mM
2	phosphate buffer		20 mM
3	NaCl		500 mM
4	DTT		5 mM
5	NaN3		0.02 %

Sample #2

Temperature 301 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
6	Ets-1	[U-99% 15N]		0.3 ~ 0.8 mM

Sample #3

Temperature 301 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
7	Ets-1	[U-98% 15N; U-98% 13C; U-97% 2H]		0.3 ~ 0.8 mM

Sample #4

Temperature 301 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
8	Ets-1	[U-99% 15N; U-10% 13C]		0.3 ~ 0.8 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.13 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.13 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.04 ppm, Outliers: 3 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.08 ppm, Outliers: 3 Detail

Protein Blocks Logo

Calculated from 25 models in PDB: 1R36, Strand ID: A Detail

Heteronucl. T₁

125 T₁ values in 1 lists
Coherence Sz, Field strength (¹H) 500 MHz, Temperature 301 (±1) K, pH 6.5 (±0.1) Detail

Heteronucl. T₂

125 T₂ values in 1 lists
Coherence S(+,-), Field strength (¹H) 500 MHz, Temperature 301 (±1) K, pH 6.5 (±0.1) Detail

Heteronucl. NOE

125 NOE values in 1 lists
Value type na, Field strength (¹H) 500 MHz, Temperature 301 (±1) K, pH 6.5 (±0.1) Detail

Heteronucl. T₁/T₂

125 T₁/T₂ values in 1 lists
Field strength (¹H) 500 MHz, Temperature 301 (±1) K, pH 6.5 (±0.1) Detail

Order parameters

124 S² values in 1 lists
Temperature 301 (±1) K, pH 6.5 (±0.1) Detail

Release date

2005-04-25

Citation

The structural and dynamic basis of Ets-1 DNA binding autoinhibition
Lee, G.M., Donaldson, L.W., Pufall, M.A., Kang, H., Pot, I., Graves, B.J., McIntosh, L.P.
J. Biol. Chem. (2005), 280, 7088-7099, PubMed 15591056 , DOI 10.1074/jbc.M410722200 , Abstract

Related entities 1. Murine Ets-1 deltaN301, : 1 : 26 : 31 entities Detail

Interaction partners 1. Murine Ets-1 deltaN301, : 3 interactors Detail

More details for 3 interactors identified by 2 citations

Experiments performed 18 experiments Detail

1 1H-15N HSQC

Instrument

#1 Model Varian Unity (500 MHz)

#2 Model Varian Inova (600 MHz)

#3 Model Varian Inova (800 MHz)

Sample #1

Temperature 301 (±1) K, pH 6.5 (±0.1), Details Samples irreversibly aggregated at NMR conditions after approximately 1 week.

#	Name	Isotope labeling	Concentration
1	Ets-1	[U-99% 15N; U-99% 13C]	0.3 ~ 0.8 mM
2	phosphate buffer		20 mM
3	NaCl		500 mM
4	DTT		5 mM
5	NaN3		0.02 %

Sample #2

Temperature 301 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
6	Ets-1	[U-99% 15N]		0.3 ~ 0.8 mM

Sample #3

Temperature 301 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
7	Ets-1	[U-98% 15N; U-98% 13C; U-97% 2H]		0.3 ~ 0.8 mM

Sample #4

Temperature 301 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
8	Ets-1	[U-99% 15N; U-10% 13C]		0.3 ~ 0.8 mM

2 1H-13C CT HSQC

Instrument

#1 Model Varian Unity (500 MHz)

#2 Model Varian Inova (600 MHz)

#3 Model Varian Inova (800 MHz)

Sample #1

Temperature 301 (±1) K, pH 6.5 (±0.1), Details Samples irreversibly aggregated at NMR conditions after approximately 1 week.

#	Name	Isotope labeling	Concentration
1	Ets-1	[U-99% 15N; U-99% 13C]	0.3 ~ 0.8 mM
2	phosphate buffer		20 mM
3	NaCl		500 mM
4	DTT		5 mM
5	NaN3		0.02 %

Sample #2

Temperature 301 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
6	Ets-1	[U-99% 15N]		0.3 ~ 0.8 mM

Sample #3

Temperature 301 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
7	Ets-1	[U-98% 15N; U-98% 13C; U-97% 2H]		0.3 ~ 0.8 mM

Sample #4

Temperature 301 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
8	Ets-1	[U-99% 15N; U-10% 13C]		0.3 ~ 0.8 mM

3 1H-15N NOESY-HSQC

Instrument

#1 Model Varian Unity (500 MHz)

#2 Model Varian Inova (600 MHz)

#3 Model Varian Inova (800 MHz)

Sample #1

Temperature 301 (±1) K, pH 6.5 (±0.1), Details Samples irreversibly aggregated at NMR conditions after approximately 1 week.

#	Name	Isotope labeling	Concentration
1	Ets-1	[U-99% 15N; U-99% 13C]	0.3 ~ 0.8 mM
2	phosphate buffer		20 mM
3	NaCl		500 mM
4	DTT		5 mM
5	NaN3		0.02 %

Sample #2

Temperature 301 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
6	Ets-1	[U-99% 15N]		0.3 ~ 0.8 mM

Sample #3

Temperature 301 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
7	Ets-1	[U-98% 15N; U-98% 13C; U-97% 2H]		0.3 ~ 0.8 mM

Sample #4

Temperature 301 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
8	Ets-1	[U-99% 15N; U-10% 13C]		0.3 ~ 0.8 mM

4 1H-15N TOCSY-HSQC

Instrument

#1 Model Varian Unity (500 MHz)

#2 Model Varian Inova (600 MHz)

#3 Model Varian Inova (800 MHz)

Sample #1

Temperature 301 (±1) K, pH 6.5 (±0.1), Details Samples irreversibly aggregated at NMR conditions after approximately 1 week.

#	Name	Isotope labeling	Concentration
1	Ets-1	[U-99% 15N; U-99% 13C]	0.3 ~ 0.8 mM
2	phosphate buffer		20 mM
3	NaCl		500 mM
4	DTT		5 mM
5	NaN3		0.02 %

Sample #2

Temperature 301 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
6	Ets-1	[U-99% 15N]		0.3 ~ 0.8 mM

Sample #3

Temperature 301 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
7	Ets-1	[U-98% 15N; U-98% 13C; U-97% 2H]		0.3 ~ 0.8 mM

Sample #4

Temperature 301 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
8	Ets-1	[U-99% 15N; U-10% 13C]		0.3 ~ 0.8 mM

5 HNCACB

Instrument

#1 Model Varian Unity (500 MHz)

#2 Model Varian Inova (600 MHz)

#3 Model Varian Inova (800 MHz)

Sample #1

Temperature 301 (±1) K, pH 6.5 (±0.1), Details Samples irreversibly aggregated at NMR conditions after approximately 1 week.

#	Name	Isotope labeling	Concentration
1	Ets-1	[U-99% 15N; U-99% 13C]	0.3 ~ 0.8 mM
2	phosphate buffer		20 mM
3	NaCl		500 mM
4	DTT		5 mM
5	NaN3		0.02 %

Sample #2

Temperature 301 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
6	Ets-1	[U-99% 15N]		0.3 ~ 0.8 mM

Sample #3

Temperature 301 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
7	Ets-1	[U-98% 15N; U-98% 13C; U-97% 2H]		0.3 ~ 0.8 mM

Sample #4

Temperature 301 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
8	Ets-1	[U-99% 15N; U-10% 13C]		0.3 ~ 0.8 mM

6 CBCA(CO)NNH

Instrument

#1 Model Varian Unity (500 MHz)

#2 Model Varian Inova (600 MHz)

#3 Model Varian Inova (800 MHz)

Sample #1

Temperature 301 (±1) K, pH 6.5 (±0.1), Details Samples irreversibly aggregated at NMR conditions after approximately 1 week.

#	Name	Isotope labeling	Concentration
1	Ets-1	[U-99% 15N; U-99% 13C]	0.3 ~ 0.8 mM
2	phosphate buffer		20 mM
3	NaCl		500 mM
4	DTT		5 mM
5	NaN3		0.02 %

Sample #2

Temperature 301 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
6	Ets-1	[U-99% 15N]		0.3 ~ 0.8 mM

Sample #3

Temperature 301 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
7	Ets-1	[U-98% 15N; U-98% 13C; U-97% 2H]		0.3 ~ 0.8 mM

Sample #4

Temperature 301 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
8	Ets-1	[U-99% 15N; U-10% 13C]		0.3 ~ 0.8 mM

7 HNHA

Instrument

#1 Model Varian Unity (500 MHz)

#2 Model Varian Inova (600 MHz)

#3 Model Varian Inova (800 MHz)

Sample #1

Temperature 301 (±1) K, pH 6.5 (±0.1), Details Samples irreversibly aggregated at NMR conditions after approximately 1 week.

#	Name	Isotope labeling	Concentration
1	Ets-1	[U-99% 15N; U-99% 13C]	0.3 ~ 0.8 mM
2	phosphate buffer		20 mM
3	NaCl		500 mM
4	DTT		5 mM
5	NaN3		0.02 %

Sample #2

Temperature 301 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
6	Ets-1	[U-99% 15N]		0.3 ~ 0.8 mM

Sample #3

Temperature 301 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
7	Ets-1	[U-98% 15N; U-98% 13C; U-97% 2H]		0.3 ~ 0.8 mM

Sample #4

Temperature 301 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
8	Ets-1	[U-99% 15N; U-10% 13C]		0.3 ~ 0.8 mM

8 HNHB

Instrument

#1 Model Varian Unity (500 MHz)

#2 Model Varian Inova (600 MHz)

#3 Model Varian Inova (800 MHz)

Sample #1

Temperature 301 (±1) K, pH 6.5 (±0.1), Details Samples irreversibly aggregated at NMR conditions after approximately 1 week.

#	Name	Isotope labeling	Concentration
1	Ets-1	[U-99% 15N; U-99% 13C]	0.3 ~ 0.8 mM
2	phosphate buffer		20 mM
3	NaCl		500 mM
4	DTT		5 mM
5	NaN3		0.02 %

Sample #2

Temperature 301 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
6	Ets-1	[U-99% 15N]		0.3 ~ 0.8 mM

Sample #3

Temperature 301 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
7	Ets-1	[U-98% 15N; U-98% 13C; U-97% 2H]		0.3 ~ 0.8 mM

Sample #4

Temperature 301 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
8	Ets-1	[U-99% 15N; U-10% 13C]		0.3 ~ 0.8 mM

9 HACAN

Instrument

#1 Model Varian Unity (500 MHz)

#2 Model Varian Inova (600 MHz)

#3 Model Varian Inova (800 MHz)

Sample #1

Temperature 301 (±1) K, pH 6.5 (±0.1), Details Samples irreversibly aggregated at NMR conditions after approximately 1 week.

#	Name	Isotope labeling	Concentration
1	Ets-1	[U-99% 15N; U-99% 13C]	0.3 ~ 0.8 mM
2	phosphate buffer		20 mM
3	NaCl		500 mM
4	DTT		5 mM
5	NaN3		0.02 %

Sample #2

Temperature 301 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
6	Ets-1	[U-99% 15N]		0.3 ~ 0.8 mM

Sample #3

Temperature 301 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
7	Ets-1	[U-98% 15N; U-98% 13C; U-97% 2H]		0.3 ~ 0.8 mM

Sample #4

Temperature 301 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
8	Ets-1	[U-99% 15N; U-10% 13C]		0.3 ~ 0.8 mM

10 HCCH-TOCSY

Instrument

#1 Model Varian Unity (500 MHz)

#2 Model Varian Inova (600 MHz)

#3 Model Varian Inova (800 MHz)

Sample #1

Temperature 301 (±1) K, pH 6.5 (±0.1), Details Samples irreversibly aggregated at NMR conditions after approximately 1 week.

#	Name	Isotope labeling	Concentration
1	Ets-1	[U-99% 15N; U-99% 13C]	0.3 ~ 0.8 mM
2	phosphate buffer		20 mM
3	NaCl		500 mM
4	DTT		5 mM
5	NaN3		0.02 %

Sample #2

Temperature 301 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
6	Ets-1	[U-99% 15N]		0.3 ~ 0.8 mM

Sample #3

Temperature 301 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
7	Ets-1	[U-98% 15N; U-98% 13C; U-97% 2H]		0.3 ~ 0.8 mM

Sample #4

Temperature 301 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
8	Ets-1	[U-99% 15N; U-10% 13C]		0.3 ~ 0.8 mM

11 HCC-TOCSY

Instrument

#1 Model Varian Unity (500 MHz)

#2 Model Varian Inova (600 MHz)

#3 Model Varian Inova (800 MHz)

Sample #1

Temperature 301 (±1) K, pH 6.5 (±0.1), Details Samples irreversibly aggregated at NMR conditions after approximately 1 week.

#	Name	Isotope labeling	Concentration
1	Ets-1	[U-99% 15N; U-99% 13C]	0.3 ~ 0.8 mM
2	phosphate buffer		20 mM
3	NaCl		500 mM
4	DTT		5 mM
5	NaN3		0.02 %

Sample #2

Temperature 301 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
6	Ets-1	[U-99% 15N]		0.3 ~ 0.8 mM

Sample #3

Temperature 301 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
7	Ets-1	[U-98% 15N; U-98% 13C; U-97% 2H]		0.3 ~ 0.8 mM

Sample #4

Temperature 301 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
8	Ets-1	[U-99% 15N; U-10% 13C]		0.3 ~ 0.8 mM

12 HNCOCA

Instrument

#1 Model Varian Unity (500 MHz)

#2 Model Varian Inova (600 MHz)

#3 Model Varian Inova (800 MHz)

Sample #1

Temperature 301 (±1) K, pH 6.5 (±0.1), Details Samples irreversibly aggregated at NMR conditions after approximately 1 week.

#	Name	Isotope labeling	Concentration
1	Ets-1	[U-99% 15N; U-99% 13C]	0.3 ~ 0.8 mM
2	phosphate buffer		20 mM
3	NaCl		500 mM
4	DTT		5 mM
5	NaN3		0.02 %

Sample #2

Temperature 301 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
6	Ets-1	[U-99% 15N]		0.3 ~ 0.8 mM

Sample #3

Temperature 301 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
7	Ets-1	[U-98% 15N; U-98% 13C; U-97% 2H]		0.3 ~ 0.8 mM

Sample #4

Temperature 301 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
8	Ets-1	[U-99% 15N; U-10% 13C]		0.3 ~ 0.8 mM

13 HNCOCACB

Instrument

#1 Model Varian Unity (500 MHz)

#2 Model Varian Inova (600 MHz)

#3 Model Varian Inova (800 MHz)

Sample #1

Temperature 301 (±1) K, pH 6.5 (±0.1), Details Samples irreversibly aggregated at NMR conditions after approximately 1 week.

#	Name	Isotope labeling	Concentration
1	Ets-1	[U-99% 15N; U-99% 13C]	0.3 ~ 0.8 mM
2	phosphate buffer		20 mM
3	NaCl		500 mM
4	DTT		5 mM
5	NaN3		0.02 %

Sample #2

Temperature 301 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
6	Ets-1	[U-99% 15N]		0.3 ~ 0.8 mM

Sample #3

Temperature 301 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
7	Ets-1	[U-98% 15N; U-98% 13C; U-97% 2H]		0.3 ~ 0.8 mM

Sample #4

Temperature 301 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
8	Ets-1	[U-99% 15N; U-10% 13C]		0.3 ~ 0.8 mM

14 HNCA

Instrument

#1 Model Varian Unity (500 MHz)

#2 Model Varian Inova (600 MHz)

#3 Model Varian Inova (800 MHz)

Sample #1

Temperature 301 (±1) K, pH 6.5 (±0.1), Details Samples irreversibly aggregated at NMR conditions after approximately 1 week.

#	Name	Isotope labeling	Concentration
1	Ets-1	[U-99% 15N; U-99% 13C]	0.3 ~ 0.8 mM
2	phosphate buffer		20 mM
3	NaCl		500 mM
4	DTT		5 mM
5	NaN3		0.02 %

Sample #2

Temperature 301 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
6	Ets-1	[U-99% 15N]		0.3 ~ 0.8 mM

Sample #3

Temperature 301 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
7	Ets-1	[U-98% 15N; U-98% 13C; U-97% 2H]		0.3 ~ 0.8 mM

Sample #4

Temperature 301 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
8	Ets-1	[U-99% 15N; U-10% 13C]		0.3 ~ 0.8 mM

15 HNCO

Instrument

#1 Model Varian Unity (500 MHz)

#2 Model Varian Inova (600 MHz)

#3 Model Varian Inova (800 MHz)

Sample #1

Temperature 301 (±1) K, pH 6.5 (±0.1), Details Samples irreversibly aggregated at NMR conditions after approximately 1 week.

#	Name	Isotope labeling	Concentration
1	Ets-1	[U-99% 15N; U-99% 13C]	0.3 ~ 0.8 mM
2	phosphate buffer		20 mM
3	NaCl		500 mM
4	DTT		5 mM
5	NaN3		0.02 %

Sample #2

Temperature 301 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
6	Ets-1	[U-99% 15N]		0.3 ~ 0.8 mM

Sample #3

Temperature 301 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
7	Ets-1	[U-98% 15N; U-98% 13C; U-97% 2H]		0.3 ~ 0.8 mM

Sample #4

Temperature 301 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
8	Ets-1	[U-99% 15N; U-10% 13C]		0.3 ~ 0.8 mM

16 EZ-HMQC-NH2

Instrument

#1 Model Varian Unity (500 MHz)

#2 Model Varian Inova (600 MHz)

#3 Model Varian Inova (800 MHz)

Sample #1

Temperature 301 (±1) K, pH 6.5 (±0.1), Details Samples irreversibly aggregated at NMR conditions after approximately 1 week.

#	Name	Isotope labeling	Concentration
1	Ets-1	[U-99% 15N; U-99% 13C]	0.3 ~ 0.8 mM
2	phosphate buffer		20 mM
3	NaCl		500 mM
4	DTT		5 mM
5	NaN3		0.02 %

Sample #2

Temperature 301 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
6	Ets-1	[U-99% 15N]		0.3 ~ 0.8 mM

Sample #3

Temperature 301 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
7	Ets-1	[U-98% 15N; U-98% 13C; U-97% 2H]		0.3 ~ 0.8 mM

Sample #4

Temperature 301 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
8	Ets-1	[U-99% 15N; U-10% 13C]		0.3 ~ 0.8 mM

17 methyl CN NOESY

Instrument

#1 Model Varian Unity (500 MHz)

#2 Model Varian Inova (600 MHz)

#3 Model Varian Inova (800 MHz)

Sample #1

Temperature 301 (±1) K, pH 6.5 (±0.1), Details Samples irreversibly aggregated at NMR conditions after approximately 1 week.

#	Name	Isotope labeling	Concentration
1	Ets-1	[U-99% 15N; U-99% 13C]	0.3 ~ 0.8 mM
2	phosphate buffer		20 mM
3	NaCl		500 mM
4	DTT		5 mM
5	NaN3		0.02 %

Sample #2

Temperature 301 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
6	Ets-1	[U-99% 15N]		0.3 ~ 0.8 mM

Sample #3

Temperature 301 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
7	Ets-1	[U-98% 15N; U-98% 13C; U-97% 2H]		0.3 ~ 0.8 mM

Sample #4

Temperature 301 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
8	Ets-1	[U-99% 15N; U-10% 13C]		0.3 ~ 0.8 mM

18 simultaneous CN NOESY

Instrument

#1 Model Varian Unity (500 MHz)

#2 Model Varian Inova (600 MHz)

#3 Model Varian Inova (800 MHz)

Sample #1

Temperature 301 (±1) K, pH 6.5 (±0.1), Details Samples irreversibly aggregated at NMR conditions after approximately 1 week.

#	Name	Isotope labeling	Concentration
1	Ets-1	[U-99% 15N; U-99% 13C]	0.3 ~ 0.8 mM
2	phosphate buffer		20 mM
3	NaCl		500 mM
4	DTT		5 mM
5	NaN3		0.02 %

Sample #2

Temperature 301 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
6	Ets-1	[U-99% 15N]		0.3 ~ 0.8 mM

Sample #3

Temperature 301 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
7	Ets-1	[U-98% 15N; U-98% 13C; U-97% 2H]		0.3 ~ 0.8 mM

Sample #4

Temperature 301 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
8	Ets-1	[U-99% 15N; U-10% 13C]		0.3 ~ 0.8 mM

nullKeywords Ets-1, ETS domain, transcription factor, DNA-binding, autoinhibition, protein dynamics, nuclear magnetic resonance

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BMRB: 5991

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Chemical shift reference

Chemical shift reference

Chemical shift reference

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Related entities 1. Murine Ets-1 deltaN301, : 1 : 26 : 31 entities Detail

Interaction partners 1. Murine Ets-1 deltaN301, : 3 interactors Detail

Experiments performed 18 experiments Detail

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