BMRBj - BMREntryMaster

Found 1 Document (2.692 seconds)

BMRB: 5992

2A4J

1H, 13C and 15N resonance assignment and secondary structure of the C-terminal domain of the human centrin 2 in complex with a 17 residue peptide from Xeroderma Pigmentosum group C protein

Authors

Miron, S., Yang, A., Blouquit, Y., Duchambon, P., Craescu, C.T.

Assembly

C-terminal domain of human centrin 2-Ca2+ - P1-XPC Peptide Complex

Entity

1. C-terminal domain of human centrin 2 (polymer, Thiol state: not present), 79 monomers, 9220.201 Da Detail

TQKMSEKDTK EEILKAFKLF DDDETGKISF KNLKRVAKEL GENLTDEELQ EMIDEADRDG DGEVSEQEFL RIMKKTSLY

2. P1-XPC peptide (polymer), 17 monomers, 2107.590 Da Detail

NWKLLAKGLL IRERLKR

3. CA (non-polymer), 40.078 Da

Total weight

11367.869 Da

Max. entity weight

9220.201 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete1

Sequence coverage: 83.3 %, Completeness: 70.9 %, Completeness (bb): 69.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	70.9 % (840 of 1185)	79.4 % (500 of 630)	56.9 % (257 of 452)	80.6 % (83 of 103)
Backbone	69.6 % (401 of 576)	82.2 % (162 of 197)	56.5 % (160 of 283)	82.3 % (79 of 96)
Sidechain	73.3 % (513 of 700)	78.1 % (338 of 433)	65.8 % (171 of 260)	57.1 % (4 of 7)
Aromatic	40.0 % (24 of 60)	80.0 % (24 of 30)	0.0 % (0 of 29)	0.0 % (0 of 1)
Methyl	73.5 % (72 of 98)	73.5 % (36 of 49)	73.5 % (36 of 49)

1. C-terminal domain of human centrin 2

TQKMSEKDTK EEILKAFKLF DDDETGKISF KNLKRVAKEL GENLTDEELQ EMIDEADRDG DGEVSEQEFL RIMKKTSLY

2. P1-XPC peptide

NWKLLAKGLL IRERLKR

Show sample condition

Sample #1

Temperature 308 (±1) K, pH 6.5 (±0.2)

#	Name	Concentration
1	C-terminal domain of human centrin 2	1.2 mM
2	P1-XPC peptide	1.2 mM
3	H2O	93 %
4	D2O	7 %

Sample #2

Temperature 308 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
5	C-terminal domain of human centrin 2	[U-15N]	1.2 mM
6	P1-XPC peptide	[U-15N]	1.2 mM
7	H2O		93 %
8	D2O		7 %

Sample #3

Temperature 308 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
9	C-terminal domain of human centrin 2	[U-13C; U-15N]	1.2 mM
10	P1-XPC peptide	[U-13C; U-15N]	1.2 mM
11	H2O		93 %
12	D2O		7 %

Chem. Shift Complete2

Sequence coverage: 32.3 %, Completeness: 41.1 %, Completeness (bb): 41.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	41.1 % (975 of 2370)	46.6 % (587 of 1260)	31.9 % (288 of 904)	48.5 % (100 of 206)
Backbone	41.4 % (477 of 1152)	52.0 % (205 of 394)	31.3 % (177 of 566)	49.5 % (95 of 192)
Sidechain	41.4 % (580 of 1400)	44.1 % (382 of 866)	37.1 % (193 of 520)	35.7 % (5 of 14)
Aromatic	25.0 % (30 of 120)	50.0 % (30 of 60)	0.0 % (0 of 58)	0.0 % (0 of 2)
Methyl	41.8 % (82 of 196)	42.9 % (42 of 98)	40.8 % (40 of 98)

1. C-terminal domain of human centrin 2

TQKMSEKDTK EEILKAFKLF DDDETGKISF KNLKRVAKEL GENLTDEELQ EMIDEADRDG DGEVSEQEFL RIMKKTSLY

2. P1-XPC peptide

NWKLLAKGLL IRERLKR

Show sample condition

Sample #1

Temperature 308 (±1) K, pH 6.5 (±0.2)

#	Name	Concentration
1	C-terminal domain of human centrin 2	1.2 mM
2	P1-XPC peptide	1.2 mM
3	H2O	93 %
4	D2O	7 %

Sample #2

Temperature 308 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
5	C-terminal domain of human centrin 2	[U-15N]	1.2 mM
6	P1-XPC peptide	[U-15N]	1.2 mM
7	H2O		93 %
8	D2O		7 %

Sample #3

Temperature 308 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
9	C-terminal domain of human centrin 2	[U-13C; U-15N]	1.2 mM
10	P1-XPC peptide	[U-13C; U-15N]	1.2 mM
11	H2O		93 %
12	D2O		7 %

Protein Blocks Logo

Calculated from 20 models in PDB: 2A4J, Strand ID: A, B Detail

Release date

2004-05-14

Citation

Xeroderma pigmentosum group C protein possesses a high affinity binding site to human centrin 2 and calmodulin
Miron, S., Yang, A., Blouquit, Y., Duchambon, P., Craescu, C.T.
J. Biol. Chem. (2003), 278, 40252-40261, PubMed 12890685 , DOI 10.1074/jbc.M302546200 , Abstract

Related entities 1. C-terminal domain of human centrin 2, : 1 : 3 : 3 : 2 : 359 entities Detail

Related entities 2. P1-XPC peptide, : 1 : 4 : 8 entities Detail

Interaction partners 2. P1-XPC peptide, : 26 interactors Detail

More details for 26 interactors identified by 15 citations

Experiments performed 9 experiments Detail

1 2D 1H COSY

Instrument

Model Varian Unity (500 MHz)

Sample #1

Temperature 308 (±1) K, pH 6.5 (±0.2)

#	Name	Concentration
1	C-terminal domain of human centrin 2	1.2 mM
2	P1-XPC peptide	1.2 mM
3	H2O	93 %
4	D2O	7 %

Sample #2

Temperature 308 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
5	C-terminal domain of human centrin 2	[U-15N]	1.2 mM
6	P1-XPC peptide	[U-15N]	1.2 mM
7	H2O		93 %
8	D2O		7 %

Sample #3

Temperature 308 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
9	C-terminal domain of human centrin 2	[U-13C; U-15N]	1.2 mM
10	P1-XPC peptide	[U-13C; U-15N]	1.2 mM
11	H2O		93 %
12	D2O		7 %

2 TOCSY

Instrument

Model Varian Unity (500 MHz)

Sample #1

Temperature 308 (±1) K, pH 6.5 (±0.2)

#	Name	Concentration
1	C-terminal domain of human centrin 2	1.2 mM
2	P1-XPC peptide	1.2 mM
3	H2O	93 %
4	D2O	7 %

Sample #2

Temperature 308 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
5	C-terminal domain of human centrin 2	[U-15N]	1.2 mM
6	P1-XPC peptide	[U-15N]	1.2 mM
7	H2O		93 %
8	D2O		7 %

Sample #3

Temperature 308 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
9	C-terminal domain of human centrin 2	[U-13C; U-15N]	1.2 mM
10	P1-XPC peptide	[U-13C; U-15N]	1.2 mM
11	H2O		93 %
12	D2O		7 %

3 NOESY

Instrument

Model Varian Unity (500 MHz)

Sample #1

Temperature 308 (±1) K, pH 6.5 (±0.2)

#	Name	Concentration
1	C-terminal domain of human centrin 2	1.2 mM
2	P1-XPC peptide	1.2 mM
3	H2O	93 %
4	D2O	7 %

Sample #2

Temperature 308 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
5	C-terminal domain of human centrin 2	[U-15N]	1.2 mM
6	P1-XPC peptide	[U-15N]	1.2 mM
7	H2O		93 %
8	D2O		7 %

Sample #3

Temperature 308 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
9	C-terminal domain of human centrin 2	[U-13C; U-15N]	1.2 mM
10	P1-XPC peptide	[U-13C; U-15N]	1.2 mM
11	H2O		93 %
12	D2O		7 %

4 1H-15N 2D HSQC

Instrument

Model Varian Unity (500 MHz)

Sample #1

Temperature 308 (±1) K, pH 6.5 (±0.2)

#	Name	Concentration
1	C-terminal domain of human centrin 2	1.2 mM
2	P1-XPC peptide	1.2 mM
3	H2O	93 %
4	D2O	7 %

Sample #2

Temperature 308 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
5	C-terminal domain of human centrin 2	[U-15N]	1.2 mM
6	P1-XPC peptide	[U-15N]	1.2 mM
7	H2O		93 %
8	D2O		7 %

Sample #3

Temperature 308 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
9	C-terminal domain of human centrin 2	[U-13C; U-15N]	1.2 mM
10	P1-XPC peptide	[U-13C; U-15N]	1.2 mM
11	H2O		93 %
12	D2O		7 %

5 3D NOESY-HSQC

Instrument

Model Varian Unity (500 MHz)

Sample #1

Temperature 308 (±1) K, pH 6.5 (±0.2)

#	Name	Concentration
1	C-terminal domain of human centrin 2	1.2 mM
2	P1-XPC peptide	1.2 mM
3	H2O	93 %
4	D2O	7 %

Sample #2

Temperature 308 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
5	C-terminal domain of human centrin 2	[U-15N]	1.2 mM
6	P1-XPC peptide	[U-15N]	1.2 mM
7	H2O		93 %
8	D2O		7 %

Sample #3

Temperature 308 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
9	C-terminal domain of human centrin 2	[U-13C; U-15N]	1.2 mM
10	P1-XPC peptide	[U-13C; U-15N]	1.2 mM
11	H2O		93 %
12	D2O		7 %

6 3D TOCSY-HSQC

Instrument

Model Varian Unity (500 MHz)

Sample #1

Temperature 308 (±1) K, pH 6.5 (±0.2)

#	Name	Concentration
1	C-terminal domain of human centrin 2	1.2 mM
2	P1-XPC peptide	1.2 mM
3	H2O	93 %
4	D2O	7 %

Sample #2

Temperature 308 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
5	C-terminal domain of human centrin 2	[U-15N]	1.2 mM
6	P1-XPC peptide	[U-15N]	1.2 mM
7	H2O		93 %
8	D2O		7 %

Sample #3

Temperature 308 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
9	C-terminal domain of human centrin 2	[U-13C; U-15N]	1.2 mM
10	P1-XPC peptide	[U-13C; U-15N]	1.2 mM
11	H2O		93 %
12	D2O		7 %

7 3D HNCA

Instrument

Model Varian Unity (500 MHz)

Sample #1

Temperature 308 (±1) K, pH 6.5 (±0.2)

#	Name	Concentration
1	C-terminal domain of human centrin 2	1.2 mM
2	P1-XPC peptide	1.2 mM
3	H2O	93 %
4	D2O	7 %

Sample #2

Temperature 308 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
5	C-terminal domain of human centrin 2	[U-15N]	1.2 mM
6	P1-XPC peptide	[U-15N]	1.2 mM
7	H2O		93 %
8	D2O		7 %

Sample #3

Temperature 308 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
9	C-terminal domain of human centrin 2	[U-13C; U-15N]	1.2 mM
10	P1-XPC peptide	[U-13C; U-15N]	1.2 mM
11	H2O		93 %
12	D2O		7 %

8 HN(CO)CA

Instrument

Model Varian Unity (500 MHz)

Sample #1

Temperature 308 (±1) K, pH 6.5 (±0.2)

#	Name	Concentration
1	C-terminal domain of human centrin 2	1.2 mM
2	P1-XPC peptide	1.2 mM
3	H2O	93 %
4	D2O	7 %

Sample #2

Temperature 308 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
5	C-terminal domain of human centrin 2	[U-15N]	1.2 mM
6	P1-XPC peptide	[U-15N]	1.2 mM
7	H2O		93 %
8	D2O		7 %

Sample #3

Temperature 308 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
9	C-terminal domain of human centrin 2	[U-13C; U-15N]	1.2 mM
10	P1-XPC peptide	[U-13C; U-15N]	1.2 mM
11	H2O		93 %
12	D2O		7 %

9 HCCH-TOCSY

Instrument

Model Varian Unity (500 MHz)

Sample #1

Temperature 308 (±1) K, pH 6.5 (±0.2)

#	Name	Concentration
1	C-terminal domain of human centrin 2	1.2 mM
2	P1-XPC peptide	1.2 mM
3	H2O	93 %
4	D2O	7 %

Sample #2

Temperature 308 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
5	C-terminal domain of human centrin 2	[U-15N]	1.2 mM
6	P1-XPC peptide	[U-15N]	1.2 mM
7	H2O		93 %
8	D2O		7 %

Sample #3

Temperature 308 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
9	C-terminal domain of human centrin 2	[U-13C; U-15N]	1.2 mM
10	P1-XPC peptide	[U-13C; U-15N]	1.2 mM
11	H2O		93 %
12	D2O		7 %

nullKeywords NMR, nuclear magnetic resonance, human centrin 2, resonance assignment, xeroderma pigmentosum group C protein

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Found 1 Document (2.692 seconds)

BMRB: 5992

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Related entities 1. C-terminal domain of human centrin 2, : 1 : 3 : 3 : 2 : 359 entities Detail

Related entities 2. P1-XPC peptide, : 1 : 4 : 8 entities Detail

Interaction partners 2. P1-XPC peptide, : 26 interactors Detail

Experiments performed 9 experiments Detail

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