BMRB: 5994

Solution structure of Human Orexin-A:Regulator of Appetite and Wakefulness

Authors

Kim, H., Hong, E., Kim, J., Lee, W.

Assembly

Orexin-A

Entity

1. Orexin-A (polymer, Thiol state: all disulfide bound), 33 monomers, 3583.149 Da Detail

QPLPDCCRQK TCSCRLYELL HGAGNHAAGI LTL

Formula weight

3583.149 Da

Entity Connection

disulfide 2 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS6:SG	1:CYS12:SG
2	disulfide	sing	1:CYS7:SG	1:CYS14:SG

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

distance geometry, simulated annealing, molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 69.3 %, Completeness (bb): 95.5 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	69.3 % (131 of 189)	69.3 % (131 of 189)
Backbone	95.5 % (64 of 67)	95.5 % (64 of 67)
Sidechain	54.9 % (67 of 122)	54.9 % (67 of 122)
Aromatic	25.0 % (2 of 8)	25.0 % (2 of 8)
Methyl	73.7 % (14 of 19)	73.7 % (14 of 19)

1. human orexin-A

QPLPDCCRQK TCSCRLYELL HGAGNHAAGI LTL

Show sample condition

Sample

Pressure 1 atm, Temperature 278 (±1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	human orexin-A			2 mM
2	phosphate buffer			50 mM
3	H2O			90 %
4	D2O			10 %

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Calculated from 1 models in PDB: 1R02, Strand ID: A Detail

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Release date

2003-11-04

Citation

Solution structure of human orexin-A: regulator of appetite and wakefulness
Kim, H., Hong, E., Kim, J., Lee, W.
J. Biochem. Mol. Biol. (2004), 37, 565-573, PubMed 15479620 , DOI: , Abstract

Related entities 1. Orexin-A, : 1 : 10 : 1 entities Detail

Experiments performed 3 experiments Detail