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BMRB: 6001

1XWE

1H, 15N and 13C resonance assignments of the C345C domain of the complement component C5

Authors

Bramham, J., Thai, C., Mark, R., Uhrin, D., Assa-Munt, N., Ogata, R.T., Barlow, P.N.

Assembly

C5 C345C domain

Entity

1. C5 C345C domain (polymer, Thiol state: not reported), 151 monomers, 17129.38 Da Detail

GSHMADCGQM QEELDLTISA ETRKQTACKP EIAYAYKVSI TSITVENVFV KYKATLLDIY KTGEAVAEKD SEITFIKKVT CTNAELVKGR QYLIMGKEAL QIKYNASFRY IYPLDSLTWI EYWPRDTTCS SCQAFLANLD EFAEDIFLNG C

Formula weight

17129.38 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

simulated anneailing, restrained molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.0 %, Completeness: 87.1 %, Completeness (bb): 83.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	87.1 % (1545 of 1773)	92.8 % (847 of 913)	77.4 % (541 of 699)	97.5 % (157 of 161)
Backbone	83.2 % (749 of 900)	94.1 % (287 of 305)	71.1 % (318 of 447)	97.3 % (144 of 148)
Sidechain	92.1 % (938 of 1018)	92.1 % (560 of 608)	91.9 % (365 of 397)	100.0 % (13 of 13)
Aromatic	72.5 % (116 of 160)	77.5 % (62 of 80)	66.7 % (52 of 78)	100.0 % (2 of 2)
Methyl	99.4 % (177 of 178)	98.9 % (88 of 89)	100.0 % (89 of 89)

1. C terminal domain of C5

GSHMADCGQM QEELDLTISA ETRKQTACKP EIAYAYKVSI TSITVENVFV KYKATLLDIY KTGEAVAEKD SEITFIKKVT CTNAELVKGR QYLIMGKEAL QIKYNASFRY IYPLDSLTWI EYWPRDTTCS SCQAFLANLD EFAEDIFLNG C

Show sample condition

Sample

Temperature 303 (±0.5) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Concentration
1	C terminal domain of C5	[U->98% 13C; U->98% 15N]	0.5 ~ 1.0 mM
2	NaCl		100 mM
3	Na phosphate		20 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	H2O	protons	4.766 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
¹³C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

Referencing offset: -0.05 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	H2O	protons	4.766 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
¹³C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

Referencing offset: -0.05 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	H2O	protons	4.766 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
¹³C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

Referencing offset: -0.04 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 40 models in PDB: 1XWE, Strand ID: A Detail

Release date

2004-04-06

Citation

1H, 15N and 13C resonance assignments of the C345C domain of the complement component C5
Bramham, J., Rance, M., Thai, C., Uhrin, D., Assa-Munt, N., Ogata, R.T., Barlow, P.N.
J. Biomol. NMR (2004), 29, 217-218, PubMed 15014240 , DOI 10.1023/B:JNMR.0000019243.25787.15 , Abstract

Related entities 1. C5 C345C domain, : 1 : 17 : 77 entities Detail

Interaction partners 1. C5 C345C domain, : 4 interactors Detail

More details for 4 interactors identified by 3 citations

Experiments performed 11 experiments Detail

1 1H,15N-HSQC