BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	94.5 % (829 of 877)	94.7 % (427 of 451)	93.7 % (329 of 351)	97.3 % (73 of 75)
Backbone	97.0 % (427 of 440)	97.3 % (144 of 148)	96.8 % (215 of 222)	97.1 % (68 of 70)
Sidechain	92.7 % (472 of 509)	93.4 % (283 of 303)	91.5 % (184 of 201)	100.0 % (5 of 5)
Aromatic	85.0 % (85 of 100)	90.0 % (45 of 50)	79.6 % (39 of 49)	100.0 % (1 of 1)
Methyl	100.0 % (82 of 82)	100.0 % (41 of 41)	100.0 % (41 of 41)

1. polymer

PPPYPTDCSI VSFLARLGCS SCLDYFTTQG LTTIYQIEHY SMDDLASLKI PEQFRHAIWK GILDHRQLHE FAAAS

Show sample condition

Sample #1

Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Concentration
1	polymer	[U-15N]	0.75 mM
2	sodium posphate		25 mM
3	sodium chloride		150 mM
4	AEBSF		1 mM
5	DTT		3 mM

Sample #2

Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Concentration
6	polymer	[U-13C; U-15N]	0.25 mM
7	sodium posphate		25 mM
8	sodium chloride		150 mM
9	AEBSF		1 mM
10	DTT		3 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.09 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.09 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.05 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.49 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 1RG6, Strand ID: A Detail

Release date

2007-03-21

Citation

NMR structure of the p63 SAM domain and dynamical properties of G534V and T537P pathological mutants, identified in the AEC syndrome
Cicero, D.O., Falconi, M., Candi, E., Mele, S., Cadot, B., Di Venere, A., Rufini, S., Melino, G., Desideri, A.
Cell Biochem. Biophys. (2006), 44, 475-489, PubMed 16679535 , DOI 10.1385/CBB:44:3:475 , Abstract

Related entities 1. p63 monomer, : 1 : 22 entities Detail

Interaction partners 1. p63 monomer, : 19 interactors Detail

More details for 19 interactors identified by 13 citations

Experiments performed 17 experiments Detail

1 2D 1H-15N HSQC

Instrument

#1 Model Bruker Avance (700 MHz)

#2 Model Bruker Avance (400 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Concentration
1	polymer	[U-15N]	0.75 mM
2	sodium posphate		25 mM
3	sodium chloride		150 mM
4	AEBSF		1 mM
5	DTT		3 mM

Sample #2

Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Concentration
6	polymer	[U-13C; U-15N]	0.25 mM
7	sodium posphate		25 mM
8	sodium chloride		150 mM
9	AEBSF		1 mM
10	DTT		3 mM

2 3D 1H-1H-15N TOCSY

Instrument

#1 Model Bruker Avance (700 MHz)

#2 Model Bruker Avance (400 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Concentration
1	polymer	[U-15N]	0.75 mM
2	sodium posphate		25 mM
3	sodium chloride		150 mM
4	AEBSF		1 mM
5	DTT		3 mM

Sample #2

Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Concentration
6	polymer	[U-13C; U-15N]	0.25 mM
7	sodium posphate		25 mM
8	sodium chloride		150 mM
9	AEBSF		1 mM
10	DTT		3 mM

3 3D 1H-1H-15N NOESY

Instrument

#1 Model Bruker Avance (700 MHz)

#2 Model Bruker Avance (400 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Concentration
1	polymer	[U-15N]	0.75 mM
2	sodium posphate		25 mM
3	sodium chloride		150 mM
4	AEBSF		1 mM
5	DTT		3 mM

Sample #2

Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Concentration
6	polymer	[U-13C; U-15N]	0.25 mM
7	sodium posphate		25 mM
8	sodium chloride		150 mM
9	AEBSF		1 mM
10	DTT		3 mM

4 3D HNHA

Instrument

#1 Model Bruker Avance (700 MHz)

#2 Model Bruker Avance (400 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Concentration
1	polymer	[U-15N]	0.75 mM
2	sodium posphate		25 mM
3	sodium chloride		150 mM
4	AEBSF		1 mM
5	DTT		3 mM

Sample #2

Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Concentration
6	polymer	[U-13C; U-15N]	0.25 mM
7	sodium posphate		25 mM
8	sodium chloride		150 mM
9	AEBSF		1 mM
10	DTT		3 mM

5 3D HNCA

Instrument

#1 Model Bruker Avance (700 MHz)

#2 Model Bruker Avance (400 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Concentration
1	polymer	[U-15N]	0.75 mM
2	sodium posphate		25 mM
3	sodium chloride		150 mM
4	AEBSF		1 mM
5	DTT		3 mM

Sample #2

Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Concentration
6	polymer	[U-13C; U-15N]	0.25 mM
7	sodium posphate		25 mM
8	sodium chloride		150 mM
9	AEBSF		1 mM
10	DTT		3 mM

6 3D HNCO

Instrument

#1 Model Bruker Avance (700 MHz)

#2 Model Bruker Avance (400 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Concentration
1	polymer	[U-15N]	0.75 mM
2	sodium posphate		25 mM
3	sodium chloride		150 mM
4	AEBSF		1 mM
5	DTT		3 mM

Sample #2

Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Concentration
6	polymer	[U-13C; U-15N]	0.25 mM
7	sodium posphate		25 mM
8	sodium chloride		150 mM
9	AEBSF		1 mM
10	DTT		3 mM

7 3D CBCA(co)NH

Instrument

#1 Model Bruker Avance (700 MHz)

#2 Model Bruker Avance (400 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Concentration
1	polymer	[U-15N]	0.75 mM
2	sodium posphate		25 mM
3	sodium chloride		150 mM
4	AEBSF		1 mM
5	DTT		3 mM

Sample #2

Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Concentration
6	polymer	[U-13C; U-15N]	0.25 mM
7	sodium posphate		25 mM
8	sodium chloride		150 mM
9	AEBSF		1 mM
10	DTT		3 mM

8 3D CBCANH

Instrument

#1 Model Bruker Avance (700 MHz)

#2 Model Bruker Avance (400 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Concentration
1	polymer	[U-15N]	0.75 mM
2	sodium posphate		25 mM
3	sodium chloride		150 mM
4	AEBSF		1 mM
5	DTT		3 mM

Sample #2

Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Concentration
6	polymer	[U-13C; U-15N]	0.25 mM
7	sodium posphate		25 mM
8	sodium chloride		150 mM
9	AEBSF		1 mM
10	DTT		3 mM

9 3D HBHA(co)NH

Instrument

#1 Model Bruker Avance (700 MHz)

#2 Model Bruker Avance (400 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Concentration
1	polymer	[U-15N]	0.75 mM
2	sodium posphate		25 mM
3	sodium chloride		150 mM
4	AEBSF		1 mM
5	DTT		3 mM

Sample #2

Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Concentration
6	polymer	[U-13C; U-15N]	0.25 mM
7	sodium posphate		25 mM
8	sodium chloride		150 mM
9	AEBSF		1 mM
10	DTT		3 mM

10 3D HACACO

Instrument

#1 Model Bruker Avance (700 MHz)

#2 Model Bruker Avance (400 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Concentration
1	polymer	[U-15N]	0.75 mM
2	sodium posphate		25 mM
3	sodium chloride		150 mM
4	AEBSF		1 mM
5	DTT		3 mM

Sample #2

Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Concentration
6	polymer	[U-13C; U-15N]	0.25 mM
7	sodium posphate		25 mM
8	sodium chloride		150 mM
9	AEBSF		1 mM
10	DTT		3 mM

11 2D HACA

Instrument

#1 Model Bruker Avance (700 MHz)

#2 Model Bruker Avance (400 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Concentration
1	polymer	[U-15N]	0.75 mM
2	sodium posphate		25 mM
3	sodium chloride		150 mM
4	AEBSF		1 mM
5	DTT		3 mM

Sample #2

Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Concentration
6	polymer	[U-13C; U-15N]	0.25 mM
7	sodium posphate		25 mM
8	sodium chloride		150 mM
9	AEBSF		1 mM
10	DTT		3 mM

12 3D hCCH TOCSY

Instrument

#1 Model Bruker Avance (700 MHz)

#2 Model Bruker Avance (400 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Concentration
1	polymer	[U-15N]	0.75 mM
2	sodium posphate		25 mM
3	sodium chloride		150 mM
4	AEBSF		1 mM
5	DTT		3 mM

Sample #2

Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Concentration
6	polymer	[U-13C; U-15N]	0.25 mM
7	sodium posphate		25 mM
8	sodium chloride		150 mM
9	AEBSF		1 mM
10	DTT		3 mM

13 3D HcCH TOCSY

Instrument

#1 Model Bruker Avance (700 MHz)

#2 Model Bruker Avance (400 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Concentration
1	polymer	[U-15N]	0.75 mM
2	sodium posphate		25 mM
3	sodium chloride		150 mM
4	AEBSF		1 mM
5	DTT		3 mM

Sample #2

Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Concentration
6	polymer	[U-13C; U-15N]	0.25 mM
7	sodium posphate		25 mM
8	sodium chloride		150 mM
9	AEBSF		1 mM
10	DTT		3 mM

14 3D HcCH COSY

Instrument

#1 Model Bruker Avance (700 MHz)

#2 Model Bruker Avance (400 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Concentration
1	polymer	[U-15N]	0.75 mM
2	sodium posphate		25 mM
3	sodium chloride		150 mM
4	AEBSF		1 mM
5	DTT		3 mM

Sample #2

Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Concentration
6	polymer	[U-13C; U-15N]	0.25 mM
7	sodium posphate		25 mM
8	sodium chloride		150 mM
9	AEBSF		1 mM
10	DTT		3 mM

15 3D hCCH COSY

Instrument

#1 Model Bruker Avance (700 MHz)

#2 Model Bruker Avance (400 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Concentration
1	polymer	[U-15N]	0.75 mM
2	sodium posphate		25 mM
3	sodium chloride		150 mM
4	AEBSF		1 mM
5	DTT		3 mM

Sample #2

Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Concentration
6	polymer	[U-13C; U-15N]	0.25 mM
7	sodium posphate		25 mM
8	sodium chloride		150 mM
9	AEBSF		1 mM
10	DTT		3 mM

16 3D C13,N15 edited NOESY

Instrument

#1 Model Bruker Avance (700 MHz)

#2 Model Bruker Avance (400 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Concentration
1	polymer	[U-15N]	0.75 mM
2	sodium posphate		25 mM
3	sodium chloride		150 mM
4	AEBSF		1 mM
5	DTT		3 mM

Sample #2

Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Concentration
6	polymer	[U-13C; U-15N]	0.25 mM
7	sodium posphate		25 mM
8	sodium chloride		150 mM
9	AEBSF		1 mM
10	DTT		3 mM

17 2D 1H-13C HSQC

Instrument

#1 Model Bruker Avance (700 MHz)

#2 Model Bruker Avance (400 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Concentration
1	polymer	[U-15N]	0.75 mM
2	sodium posphate		25 mM
3	sodium chloride		150 mM
4	AEBSF		1 mM
5	DTT		3 mM

Sample #2

Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Concentration
6	polymer	[U-13C; U-15N]	0.25 mM
7	sodium posphate		25 mM
8	sodium chloride		150 mM
9	AEBSF		1 mM
10	DTT		3 mM

nullKeywords NMR spectroscopy, p53, SAM domain, tumor suppressor

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Found 1 Document (2.032 seconds)

BMRB: 6002

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Sample #2

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. p63 monomer, : 1 : 22 entities Detail

Interaction partners 1. p63 monomer, : 19 interactors Detail

Experiments performed 17 experiments Detail

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