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Found 1 Document (6.927 seconds)

BMRB: 6004

1Q8G

1H, 13C, 15N assignments of human Cofilin

Authors

Zierler-Gould, K.M., Pope, B.J., Weeds, A.G., Ball, L.J.

Assembly

Cofilin, non-muscle isoform

Entity

1. Cofilin, non-muscle isoform (polymer, Thiol state: all free), 166 monomers, 18502.27 Da Detail

MASGVAVSDG VIKVFNDMKV RKSSTPEEVK KRKKAVLFCL SEDKKNIILE EGKEILVGDV GQTVDDPYAT FVKMLPDKDC RYALYDATYE TKESKKEDLV FIFWAPESAP LKSKMIYASS KDAIKKKLTG IKHELQANCY EEVKDRCTLA EKLGGSAVIS LEGKPL

Formula weight

18502.27 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.8 %, Completeness: 86.9 %, Completeness (bb): 84.5 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	86.9 % (1705 of 1961)	94.6 % (971 of 1026)	74.5 % (573 of 769)	97.0 % (161 of 166)
Backbone	84.5 % (831 of 984)	97.0 % (325 of 335)	71.6 % (350 of 489)	97.5 % (156 of 160)
Sidechain	90.9 % (1031 of 1134)	93.8 % (648 of 691)	86.3 % (377 of 437)	100.0 % (6 of 6)
Aromatic	88.6 % (101 of 114)	96.5 % (55 of 57)	80.4 % (45 of 56)	100.0 % (1 of 1)
Methyl	98.9 % (186 of 188)	98.9 % (93 of 94)	98.9 % (93 of 94)

1. cofilin

MASGVAVSDG VIKVFNDMKV RKSSTPEEVK KRKKAVLFCL SEDKKNIILE EGKEILVGDV GQTVDDPYAT FVKMLPDKDC RYALYDATYE TKESKKEDLV FIFWAPESAP LKSKMIYASS KDAIKKKLTG IKHELQANCY EEVKDRCTLA EKLGGSAVIS LEGKPL

Show sample condition

Sample #1

Pressure 1 atm, Temperature 300 (±2) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Concentration
1	cofilin	[U-15N; U-13C]	0.8 mM
2	phosphate buffer		10 mM
3	H2O		90 %
4	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 300 (±2) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Concentration
5	cofilin	[U-15N]	1 mM
6	phosphate buffer		10 mM
7	H2O		90 %
8	D2O		10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.07 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.07 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.05 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 1Q8G, Strand ID: A Detail

Release date

2004-11-14

Citation

Backbone and sidechain 1H, 13C and 15N resonance assignments of human cofilin
Zierler-Gould, K.M., Pope, B.J., Weeds, A.G., Ball, L.J.
J. Biomol. NMR (2004), 29, 429-430, PubMed 15213453 , DOI 10.1023/B:JNMR.0000032508.54841.0f , Abstract

Related entities 1. Cofilin, non-muscle isoform, : 1 : 8 : 8 : 148 entities Detail

Interaction partners 1. Cofilin, non-muscle isoform, : 102 interactors Detail

More details for 102 interactors identified by 40 citations

Experiments performed 8 experiments Detail

1 3D 13C-separated NOESY-HSQC in H2O (aliphatic centred)

Instrument

Model Bruker DMX (750 MHz)

Sample #1

Pressure 1 atm, Temperature 300 (±2) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Concentration
1	cofilin	[U-15N; U-13C]	0.8 mM
2	phosphate buffer		10 mM
3	H2O		90 %
4	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 300 (±2) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Concentration
5	cofilin	[U-15N]	1 mM
6	phosphate buffer		10 mM
7	H2O		90 %
8	D2O		10 %

2 3D 13C-separated NOESY-HSQC in H2O (aromatic centred)

Instrument

Model Bruker DMX (750 MHz)

Sample #1

Pressure 1 atm, Temperature 300 (±2) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Concentration
1	cofilin	[U-15N; U-13C]	0.8 mM
2	phosphate buffer		10 mM
3	H2O		90 %
4	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 300 (±2) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Concentration
5	cofilin	[U-15N]	1 mM
6	phosphate buffer		10 mM
7	H2O		90 %
8	D2O		10 %

3 3D 15N-separated NOESY-HSQC

Instrument

Model Bruker DMX (750 MHz)

Sample #1

Pressure 1 atm, Temperature 300 (±2) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Concentration
1	cofilin	[U-15N; U-13C]	0.8 mM
2	phosphate buffer		10 mM
3	H2O		90 %
4	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 300 (±2) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Concentration
5	cofilin	[U-15N]	1 mM
6	phosphate buffer		10 mM
7	H2O		90 %
8	D2O		10 %

4 3D HNHA

Instrument

Model Bruker DMX (750 MHz)

Sample #1

Pressure 1 atm, Temperature 300 (±2) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Concentration
1	cofilin	[U-15N; U-13C]	0.8 mM
2	phosphate buffer		10 mM
3	H2O		90 %
4	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 300 (±2) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Concentration
5	cofilin	[U-15N]	1 mM
6	phosphate buffer		10 mM
7	H2O		90 %
8	D2O		10 %

5 3D HNHB

Instrument

Model Bruker DMX (750 MHz)

Sample #1

Pressure 1 atm, Temperature 300 (±2) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Concentration
1	cofilin	[U-15N; U-13C]	0.8 mM
2	phosphate buffer		10 mM
3	H2O		90 %
4	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 300 (±2) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Concentration
5	cofilin	[U-15N]	1 mM
6	phosphate buffer		10 mM
7	H2O		90 %
8	D2O		10 %

6 2D NOESY

Instrument

Model Bruker DMX (750 MHz)

Sample #1

Pressure 1 atm, Temperature 300 (±2) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Concentration
1	cofilin	[U-15N; U-13C]	0.8 mM
2	phosphate buffer		10 mM
3	H2O		90 %
4	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 300 (±2) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Concentration
5	cofilin	[U-15N]	1 mM
6	phosphate buffer		10 mM
7	H2O		90 %
8	D2O		10 %

7 2D TOCSY

Instrument

Model Bruker DMX (750 MHz)

Sample #1

Pressure 1 atm, Temperature 300 (±2) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Concentration
1	cofilin	[U-15N; U-13C]	0.8 mM
2	phosphate buffer		10 mM
3	H2O		90 %
4	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 300 (±2) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Concentration
5	cofilin	[U-15N]	1 mM
6	phosphate buffer		10 mM
7	H2O		90 %
8	D2O		10 %

8 2D DQF-COSY

Instrument

Model Bruker DMX (750 MHz)

Sample #1

Pressure 1 atm, Temperature 300 (±2) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Concentration
1	cofilin	[U-15N; U-13C]	0.8 mM
2	phosphate buffer		10 mM
3	H2O		90 %
4	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 300 (±2) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Concentration
5	cofilin	[U-15N]	1 mM
6	phosphate buffer		10 mM
7	H2O		90 %
8	D2O		10 %

nullKeywords cofilin/ADF, NMR, chemical shift, actin binding, cytoskeleton

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Found 1 Document (6.927 seconds)

BMRB: 6004

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Chemical shift reference

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Chemical shift reference

Related entities 1. Cofilin, non-muscle isoform, : 1 : 8 : 8 : 148 entities Detail

Interaction partners 1. Cofilin, non-muscle isoform, : 102 interactors Detail

Experiments performed 8 experiments Detail

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