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BMRB: 6005

1RGO

Structural Basis for Recognition of the mRNA Class II AU-Rich Element by the Tandem Zinc Finger Domain of TIS11d

Authors

Hudson, B.P., Martinez-Yamout, M.A., Dyson, H.JANE., Wright, P.E.

Assembly

TIS11d TZF/ 5'-UUAUUUAUU-3' complex

Entity

1. TIS11d TZF (polymer, Thiol state: all other bound), 70 monomers, 8298.309 Da Detail

STRYKTELCR PFEESGTCKY GEKCQFAHGF HELRSLTRHP KYKTELCRTF HTIGFCPYGP RCHFIHNADE

2. 5'-UUAUUUAUU-3' (polymer, Thiol state: not present), 9 monomers, 2819.587 Da Detail

UUAUUUAUU

3. ZN (non-polymer), 65.409 × 2 Da

Total weight

11248.714 Da

Max. entity weight

8298.309 Da

Entity Connection

na 8 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS9:SG	3:ZN1:ZN
2	na	sing	1:CYS18:SG	3:ZN1:ZN
3	na	sing	1:CYS24:SG	3:ZN1:ZN
4	na	sing	1:HIS28:NE2	3:ZN1:ZN
5	na	sing	1:CYS47:SG	3:ZN1:ZN
6	na	sing	1:CYS56:SG	3:ZN1:ZN
7	na	sing	1:CYS62:SG	3:ZN1:ZN
8	na	sing	1:HIS66:NE2	3:ZN1:ZN

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

Individual zinc finger domains were created in DYANA and docked to UAUU (finger I) and UUAUU (finger II) using five rounds of simulated annealing in AMBER using distance and torsional restraints. Finger I/UAUU and Finger II/UUAUU complexes were connected to form TZF/UUAUUUAUU complexes and refined in AMBER with residual dipolar coupling restraints added. Calculations were then switched from in vacuo to Generalized Born continuum solvent model and one additional round of simulated annealing was performed.

Data set

assigned_chemical_shifts

Chem. Shift Complete1

Sequence coverage: 87.3 %, Completeness: 78.4 %, Completeness (bb): 77.5 % Detail

Polymer type: polypeptide(L) polyribonucleotide

	Total	¹H	¹³C	¹⁵N
All	78.4 % (775 of 989)	81.3 % (423 of 520)	72.6 % (286 of 394)	88.0 % (66 of 75)
Backbone	77.5 % (396 of 511)	70.8 % (138 of 195)	77.6 % (194 of 250)	97.0 % (64 of 66)
Sidechain	81.4 % (442 of 543)	87.7 % (285 of 325)	74.2 % (155 of 209)	22.2 % (2 of 9)
Aromatic	42.8 % (71 of 166)	55.4 % (46 of 83)	32.9 % (25 of 76)	0.0 % (0 of 7)
Methyl	100.0 % (42 of 42)	100.0 % (21 of 21)	100.0 % (21 of 21)

1. TIS11d TZF

STRYKTELCR PFEESGTCKY GEKCQFAHGF HELRSLTRHP KYKTELCRTF HTIGFCPYGP RCHFIHNADE

2. 5'-UUAUUUAUU-3'

UUAUUUAUU

Show sample condition

Sample #1

Temperature 298 (±0.5) K, pH 6.2 (±0.1)

#	Name	Isotope labeling	Concentration
1	TIS11d TZF	[U-15N]	0.5 mM
2	5'-UUAUUUAUU-3'		0.5 mM
3	TrisHCl		10 mM
4	KCl		20 mM
5	DTT		2.5 mM
6	ZnSO4		25 uM
7	H20		95 %
8	D2O		5 %

Sample #2

Temperature 298 (±0.5) K, pH 6.2 (±0.1)

#	Name	Isotope labeling	Concentration
17	TIS11d TZF	[U-13C; U-15N]	0.5 mM
18	5'-UUAUUUAUU-3'		0.5 mM
19	Tris-HCl		10 mM
20	KCl		20 mM
21	DTT		2.5 mM
22	ZnSO4		25 uM
23	D2O		100 %

Chem. Shift Complete2

Sequence coverage: 27.8 %, Completeness: 46.9 %, Completeness (bb): 46.2 % Detail

Polymer type: polypeptide(L) polyribonucleotide

	Total	¹H	¹³C	¹⁵N
All	46.9 % (928 of 1978)	49.5 % (515 of 1040)	42.4 % (334 of 788)	52.7 % (79 of 150)
Backbone	46.2 % (472 of 1022)	43.8 % (171 of 390)	44.8 % (224 of 500)	58.3 % (77 of 132)
Sidechain	48.9 % (531 of 1086)	52.9 % (344 of 650)	44.3 % (185 of 418)	11.1 % (2 of 18)
Aromatic	29.2 % (97 of 332)	40.4 % (67 of 166)	19.7 % (30 of 152)	0.0 % (0 of 14)
Methyl	64.3 % (54 of 84)	64.3 % (27 of 42)	64.3 % (27 of 42)

1. TIS11d TZF

STRYKTELCR PFEESGTCKY GEKCQFAHGF HELRSLTRHP KYKTELCRTF HTIGFCPYGP RCHFIHNADE

2. 5'-UUAUUUAUU-3'

UUAUUUAUU

Show sample condition

Sample #1

Temperature 298 (±0.5) K, pH 6.2 (±0.1)

#	Name	Isotope labeling	Concentration
1	TIS11d TZF	[U-15N]	0.5 mM
2	5'-UUAUUUAUU-3'		0.5 mM
3	TrisHCl		10 mM
4	KCl		20 mM
5	DTT		2.5 mM
6	ZnSO4		25 uM
7	H20		95 %
8	D2O		5 %

Sample #2

Temperature 298 (±0.5) K, pH 6.2 (±0.1)

#	Name	Isotope labeling	Concentration
17	TIS11d TZF	[U-13C; U-15N]	0.5 mM
18	5'-UUAUUUAUU-3'		0.5 mM
19	Tris-HCl		10 mM
20	KCl		20 mM
21	DTT		2.5 mM
22	ZnSO4		25 uM
23	D2O		100 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	direct	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	external	direct	0.1013291

Referencing offset: -0.01 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	direct	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	external	direct	0.1013291

Referencing offset: -0.01 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	direct	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	external	direct	0.1013291

Referencing offset: 0.12 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	direct	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	external	direct	0.1013291

Referencing offset: 0.05 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 1RGO, Strand ID: A Detail

Release date

2005-11-13

Citation 1

Recognition of the mRNA AU-rich element by the zinc finger domain of TIS11d
Hudson, B.P., Martinez-Yamout, M.A., Dyson, H.JANE., Wright, P.E.
Nat. Struct. Mol. Biol. (2004), 11, 257-264, PubMed 14981510 , DOI 10.1038/nsmb738 , Abstract

Show more 2 citaions

Citation 2

ERF-2, the human homologue of the murine Tis11d early response gene
Nie, X.F., Maclean, K.N., Kumar, V., McKay, I.A., Bustin, S.A.
Gene (1995), 152, 285-286, PubMed 7835719 , DOI: , Abstract

Citation 3

Characteristics of the interaction of a synthetic human tristetraprolin tandem zinc finger peptide with AU-rich element-containing RNA substrates
Blackshear, P.J., Lai, W.S., Kennington, E.A., Brewer, G., Wilson, G.M., Guan, X., Zhou, P.
J. Biol. Chem. (2003), 278, 19947-19955, PubMed 12639954 , DOI 10.1074/jbc.M301290200 , Abstract

Hide non-primary 2 citaions

Entries sharing articles Swiss-Prot: 1 entries Detail

Swiss-Prot: P47974 released on 1996-10-01
Title TISD_HUMAN Entity Zinc finger protein 36, C3H1 type-like 2

Related entities 1. TIS11d TZF, : 1 : 6 : 164 entities Detail

Experiments performed 27 experiments Detail

1 1H-15N HSQC

Instrument

#1 Model Bruker Avance (600 MHz)

#2 Model Bruker DMX (750 MHz)

#3 Model Bruker Avance (900 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 6.2 (±0.1)

#	Name	Isotope labeling	Concentration
1	TIS11d TZF	[U-15N]	0.5 mM
2	5'-UUAUUUAUU-3'		0.5 mM
3	TrisHCl		10 mM
4	KCl		20 mM
5	DTT		2.5 mM
6	ZnSO4		25 uM
7	H20		95 %
8	D2O		5 %

Sample #2

Temperature 298 (±0.5) K, pH 6.2 (±0.1)

#	Name	Isotope labeling	Concentration
9	TIS11d TZF	[U-13C; U-15N]	0.5 mM
10	5'-UUAUUUAUU-3'		0.5 mM
11	TrisHCl		10 mM
12	KCl		20 mM
13	DTT		2.5 mM
14	ZnSO4		25 uM
15	H20		95 %
16	D2O		5 %

Sample #3

Temperature 298 (±0.5) K, pH 6.2 (±0.1)

#	Name	Isotope labeling	Concentration
17	TIS11d TZF	[U-13C; U-15N]	0.5 mM
18	5'-UUAUUUAUU-3'		0.5 mM
19	Tris-HCl		10 mM
20	KCl		20 mM
21	DTT		2.5 mM
22	ZnSO4		25 uM
23	D2O		100 %

Sample #4

Temperature 298 (±0.5) K, pH 6.2 (±0.1)

#	Name	Isotope labeling	Concentration
24	TIS11d TZF	[U-13C; U-15N]	0.5 mM
25	5'-UUAUUUAUU-3'		0.5 mM
26	TrisHCl		10 mM
27	KCl		20 mM
28	DTT		2.5 mM
29	ZnSO4		25 uM
30	Pf1 filamentous phage		12 mg/mL
31	D2O		100 %

2 1H-15N-HMQC

Instrument

#1 Model Bruker Avance (600 MHz)

#2 Model Bruker DMX (750 MHz)

#3 Model Bruker Avance (900 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 6.2 (±0.1)

#	Name	Isotope labeling	Concentration
1	TIS11d TZF	[U-15N]	0.5 mM
2	5'-UUAUUUAUU-3'		0.5 mM
3	TrisHCl		10 mM
4	KCl		20 mM
5	DTT		2.5 mM
6	ZnSO4		25 uM
7	H20		95 %
8	D2O		5 %

Sample #2

Temperature 298 (±0.5) K, pH 6.2 (±0.1)

#	Name	Isotope labeling	Concentration
9	TIS11d TZF	[U-13C; U-15N]	0.5 mM
10	5'-UUAUUUAUU-3'		0.5 mM
11	TrisHCl		10 mM
12	KCl		20 mM
13	DTT		2.5 mM
14	ZnSO4		25 uM
15	H20		95 %
16	D2O		5 %

Sample #3

Temperature 298 (±0.5) K, pH 6.2 (±0.1)

#	Name	Isotope labeling	Concentration
17	TIS11d TZF	[U-13C; U-15N]	0.5 mM
18	5'-UUAUUUAUU-3'		0.5 mM
19	Tris-HCl		10 mM
20	KCl		20 mM
21	DTT		2.5 mM
22	ZnSO4		25 uM
23	D2O		100 %

Sample #4

Temperature 298 (±0.5) K, pH 6.2 (±0.1)

#	Name	Isotope labeling	Concentration
24	TIS11d TZF	[U-13C; U-15N]	0.5 mM
25	5'-UUAUUUAUU-3'		0.5 mM
26	TrisHCl		10 mM
27	KCl		20 mM
28	DTT		2.5 mM
29	ZnSO4		25 uM
30	Pf1 filamentous phage		12 mg/mL
31	D2O		100 %

3 1H-13C-HSQC

Instrument

#1 Model Bruker Avance (600 MHz)

#2 Model Bruker DMX (750 MHz)

#3 Model Bruker Avance (900 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 6.2 (±0.1)

#	Name	Isotope labeling	Concentration
1	TIS11d TZF	[U-15N]	0.5 mM
2	5'-UUAUUUAUU-3'		0.5 mM
3	TrisHCl		10 mM
4	KCl		20 mM
5	DTT		2.5 mM
6	ZnSO4		25 uM
7	H20		95 %
8	D2O		5 %

Sample #2

Temperature 298 (±0.5) K, pH 6.2 (±0.1)

#	Name	Isotope labeling	Concentration
9	TIS11d TZF	[U-13C; U-15N]	0.5 mM
10	5'-UUAUUUAUU-3'		0.5 mM
11	TrisHCl		10 mM
12	KCl		20 mM
13	DTT		2.5 mM
14	ZnSO4		25 uM
15	H20		95 %
16	D2O		5 %

Sample #3

Temperature 298 (±0.5) K, pH 6.2 (±0.1)

#	Name	Isotope labeling	Concentration
17	TIS11d TZF	[U-13C; U-15N]	0.5 mM
18	5'-UUAUUUAUU-3'		0.5 mM
19	Tris-HCl		10 mM
20	KCl		20 mM
21	DTT		2.5 mM
22	ZnSO4		25 uM
23	D2O		100 %

Sample #4

Temperature 298 (±0.5) K, pH 6.2 (±0.1)

#	Name	Isotope labeling	Concentration
24	TIS11d TZF	[U-13C; U-15N]	0.5 mM
25	5'-UUAUUUAUU-3'		0.5 mM
26	TrisHCl		10 mM
27	KCl		20 mM
28	DTT		2.5 mM
29	ZnSO4		25 uM
30	Pf1 filamentous phage		12 mg/mL
31	D2O		100 %

4 1H-13C-CT-HSQC

Instrument

#1 Model Bruker Avance (600 MHz)

#2 Model Bruker DMX (750 MHz)

#3 Model Bruker Avance (900 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 6.2 (±0.1)

#	Name	Isotope labeling	Concentration
1	TIS11d TZF	[U-15N]	0.5 mM
2	5'-UUAUUUAUU-3'		0.5 mM
3	TrisHCl		10 mM
4	KCl		20 mM
5	DTT		2.5 mM
6	ZnSO4		25 uM
7	H20		95 %
8	D2O		5 %

Sample #2

Temperature 298 (±0.5) K, pH 6.2 (±0.1)

#	Name	Isotope labeling	Concentration
9	TIS11d TZF	[U-13C; U-15N]	0.5 mM
10	5'-UUAUUUAUU-3'		0.5 mM
11	TrisHCl		10 mM
12	KCl		20 mM
13	DTT		2.5 mM
14	ZnSO4		25 uM
15	H20		95 %
16	D2O		5 %

Sample #3

Temperature 298 (±0.5) K, pH 6.2 (±0.1)

#	Name	Isotope labeling	Concentration
17	TIS11d TZF	[U-13C; U-15N]	0.5 mM
18	5'-UUAUUUAUU-3'		0.5 mM
19	Tris-HCl		10 mM
20	KCl		20 mM
21	DTT		2.5 mM
22	ZnSO4		25 uM
23	D2O		100 %

Sample #4

Temperature 298 (±0.5) K, pH 6.2 (±0.1)

#	Name	Isotope labeling	Concentration
24	TIS11d TZF	[U-13C; U-15N]	0.5 mM
25	5'-UUAUUUAUU-3'		0.5 mM
26	TrisHCl		10 mM
27	KCl		20 mM
28	DTT		2.5 mM
29	ZnSO4		25 uM
30	Pf1 filamentous phage		12 mg/mL
31	D2O		100 %

5 HNCACB

Instrument

#1 Model Bruker Avance (600 MHz)

#2 Model Bruker DMX (750 MHz)

#3 Model Bruker Avance (900 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 6.2 (±0.1)

#	Name	Isotope labeling	Concentration
1	TIS11d TZF	[U-15N]	0.5 mM
2	5'-UUAUUUAUU-3'		0.5 mM
3	TrisHCl		10 mM
4	KCl		20 mM
5	DTT		2.5 mM
6	ZnSO4		25 uM
7	H20		95 %
8	D2O		5 %

Sample #2

Temperature 298 (±0.5) K, pH 6.2 (±0.1)

#	Name	Isotope labeling	Concentration
9	TIS11d TZF	[U-13C; U-15N]	0.5 mM
10	5'-UUAUUUAUU-3'		0.5 mM
11	TrisHCl		10 mM
12	KCl		20 mM
13	DTT		2.5 mM
14	ZnSO4		25 uM
15	H20		95 %
16	D2O		5 %

Sample #3

Temperature 298 (±0.5) K, pH 6.2 (±0.1)

#	Name	Isotope labeling	Concentration
17	TIS11d TZF	[U-13C; U-15N]	0.5 mM
18	5'-UUAUUUAUU-3'		0.5 mM
19	Tris-HCl		10 mM
20	KCl		20 mM
21	DTT		2.5 mM
22	ZnSO4		25 uM
23	D2O		100 %

Sample #4

Temperature 298 (±0.5) K, pH 6.2 (±0.1)

#	Name	Isotope labeling	Concentration
24	TIS11d TZF	[U-13C; U-15N]	0.5 mM
25	5'-UUAUUUAUU-3'		0.5 mM
26	TrisHCl		10 mM
27	KCl		20 mM
28	DTT		2.5 mM
29	ZnSO4		25 uM
30	Pf1 filamentous phage		12 mg/mL
31	D2O		100 %

6 CBCA(CO)NH

Instrument

#1 Model Bruker Avance (600 MHz)

#2 Model Bruker DMX (750 MHz)

#3 Model Bruker Avance (900 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 6.2 (±0.1)

#	Name	Isotope labeling	Concentration
1	TIS11d TZF	[U-15N]	0.5 mM
2	5'-UUAUUUAUU-3'		0.5 mM
3	TrisHCl		10 mM
4	KCl		20 mM
5	DTT		2.5 mM
6	ZnSO4		25 uM
7	H20		95 %
8	D2O		5 %

Sample #2

Temperature 298 (±0.5) K, pH 6.2 (±0.1)

#	Name	Isotope labeling	Concentration
9	TIS11d TZF	[U-13C; U-15N]	0.5 mM
10	5'-UUAUUUAUU-3'		0.5 mM
11	TrisHCl		10 mM
12	KCl		20 mM
13	DTT		2.5 mM
14	ZnSO4		25 uM
15	H20		95 %
16	D2O		5 %

Sample #3

Temperature 298 (±0.5) K, pH 6.2 (±0.1)

#	Name	Isotope labeling	Concentration
17	TIS11d TZF	[U-13C; U-15N]	0.5 mM
18	5'-UUAUUUAUU-3'		0.5 mM
19	Tris-HCl		10 mM
20	KCl		20 mM
21	DTT		2.5 mM
22	ZnSO4		25 uM
23	D2O		100 %

Sample #4

Temperature 298 (±0.5) K, pH 6.2 (±0.1)

#	Name	Isotope labeling	Concentration
24	TIS11d TZF	[U-13C; U-15N]	0.5 mM
25	5'-UUAUUUAUU-3'		0.5 mM
26	TrisHCl		10 mM
27	KCl		20 mM
28	DTT		2.5 mM
29	ZnSO4		25 uM
30	Pf1 filamentous phage		12 mg/mL
31	D2O		100 %

7 HNCO

Instrument

#1 Model Bruker Avance (600 MHz)

#2 Model Bruker DMX (750 MHz)

#3 Model Bruker Avance (900 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 6.2 (±0.1)

#	Name	Isotope labeling	Concentration
1	TIS11d TZF	[U-15N]	0.5 mM
2	5'-UUAUUUAUU-3'		0.5 mM
3	TrisHCl		10 mM
4	KCl		20 mM
5	DTT		2.5 mM
6	ZnSO4		25 uM
7	H20		95 %
8	D2O		5 %

Sample #2

Temperature 298 (±0.5) K, pH 6.2 (±0.1)

#	Name	Isotope labeling	Concentration
9	TIS11d TZF	[U-13C; U-15N]	0.5 mM
10	5'-UUAUUUAUU-3'		0.5 mM
11	TrisHCl		10 mM
12	KCl		20 mM
13	DTT		2.5 mM
14	ZnSO4		25 uM
15	H20		95 %
16	D2O		5 %

Sample #3

Temperature 298 (±0.5) K, pH 6.2 (±0.1)

#	Name	Isotope labeling	Concentration
17	TIS11d TZF	[U-13C; U-15N]	0.5 mM
18	5'-UUAUUUAUU-3'		0.5 mM
19	Tris-HCl		10 mM
20	KCl		20 mM
21	DTT		2.5 mM
22	ZnSO4		25 uM
23	D2O		100 %

Sample #4

Temperature 298 (±0.5) K, pH 6.2 (±0.1)

#	Name	Isotope labeling	Concentration
24	TIS11d TZF	[U-13C; U-15N]	0.5 mM
25	5'-UUAUUUAUU-3'		0.5 mM
26	TrisHCl		10 mM
27	KCl		20 mM
28	DTT		2.5 mM
29	ZnSO4		25 uM
30	Pf1 filamentous phage		12 mg/mL
31	D2O		100 %

8 C(CO)NH-TOCSY

Instrument

#1 Model Bruker Avance (600 MHz)

#2 Model Bruker DMX (750 MHz)

#3 Model Bruker Avance (900 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 6.2 (±0.1)

#	Name	Isotope labeling	Concentration
1	TIS11d TZF	[U-15N]	0.5 mM
2	5'-UUAUUUAUU-3'		0.5 mM
3	TrisHCl		10 mM
4	KCl		20 mM
5	DTT		2.5 mM
6	ZnSO4		25 uM
7	H20		95 %
8	D2O		5 %

Sample #2

Temperature 298 (±0.5) K, pH 6.2 (±0.1)

#	Name	Isotope labeling	Concentration
9	TIS11d TZF	[U-13C; U-15N]	0.5 mM
10	5'-UUAUUUAUU-3'		0.5 mM
11	TrisHCl		10 mM
12	KCl		20 mM
13	DTT		2.5 mM
14	ZnSO4		25 uM
15	H20		95 %
16	D2O		5 %

Sample #3

Temperature 298 (±0.5) K, pH 6.2 (±0.1)

#	Name	Isotope labeling	Concentration
17	TIS11d TZF	[U-13C; U-15N]	0.5 mM
18	5'-UUAUUUAUU-3'		0.5 mM
19	Tris-HCl		10 mM
20	KCl		20 mM
21	DTT		2.5 mM
22	ZnSO4		25 uM
23	D2O		100 %

Sample #4

Temperature 298 (±0.5) K, pH 6.2 (±0.1)

#	Name	Isotope labeling	Concentration
24	TIS11d TZF	[U-13C; U-15N]	0.5 mM
25	5'-UUAUUUAUU-3'		0.5 mM
26	TrisHCl		10 mM
27	KCl		20 mM
28	DTT		2.5 mM
29	ZnSO4		25 uM
30	Pf1 filamentous phage		12 mg/mL
31	D2O		100 %

9 H(CCO)NH-TOCSY

Instrument

#1 Model Bruker Avance (600 MHz)

#2 Model Bruker DMX (750 MHz)

#3 Model Bruker Avance (900 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 6.2 (±0.1)

#	Name	Isotope labeling	Concentration
1	TIS11d TZF	[U-15N]	0.5 mM
2	5'-UUAUUUAUU-3'		0.5 mM
3	TrisHCl		10 mM
4	KCl		20 mM
5	DTT		2.5 mM
6	ZnSO4		25 uM
7	H20		95 %
8	D2O		5 %

Sample #2

Temperature 298 (±0.5) K, pH 6.2 (±0.1)

#	Name	Isotope labeling	Concentration
9	TIS11d TZF	[U-13C; U-15N]	0.5 mM
10	5'-UUAUUUAUU-3'		0.5 mM
11	TrisHCl		10 mM
12	KCl		20 mM
13	DTT		2.5 mM
14	ZnSO4		25 uM
15	H20		95 %
16	D2O		5 %

Sample #3

Temperature 298 (±0.5) K, pH 6.2 (±0.1)

#	Name	Isotope labeling	Concentration
17	TIS11d TZF	[U-13C; U-15N]	0.5 mM
18	5'-UUAUUUAUU-3'		0.5 mM
19	Tris-HCl		10 mM
20	KCl		20 mM
21	DTT		2.5 mM
22	ZnSO4		25 uM
23	D2O		100 %

Sample #4

Temperature 298 (±0.5) K, pH 6.2 (±0.1)

#	Name	Isotope labeling	Concentration
24	TIS11d TZF	[U-13C; U-15N]	0.5 mM
25	5'-UUAUUUAUU-3'		0.5 mM
26	TrisHCl		10 mM
27	KCl		20 mM
28	DTT		2.5 mM
29	ZnSO4		25 uM
30	Pf1 filamentous phage		12 mg/mL
31	D2O		100 %

10 CCH-COSY

Instrument

#1 Model Bruker Avance (600 MHz)

#2 Model Bruker DMX (750 MHz)

#3 Model Bruker Avance (900 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 6.2 (±0.1)

#	Name	Isotope labeling	Concentration
1	TIS11d TZF	[U-15N]	0.5 mM
2	5'-UUAUUUAUU-3'		0.5 mM
3	TrisHCl		10 mM
4	KCl		20 mM
5	DTT		2.5 mM
6	ZnSO4		25 uM
7	H20		95 %
8	D2O		5 %

Sample #2

Temperature 298 (±0.5) K, pH 6.2 (±0.1)

#	Name	Isotope labeling	Concentration
9	TIS11d TZF	[U-13C; U-15N]	0.5 mM
10	5'-UUAUUUAUU-3'		0.5 mM
11	TrisHCl		10 mM
12	KCl		20 mM
13	DTT		2.5 mM
14	ZnSO4		25 uM
15	H20		95 %
16	D2O		5 %

Sample #3

Temperature 298 (±0.5) K, pH 6.2 (±0.1)

#	Name	Isotope labeling	Concentration
17	TIS11d TZF	[U-13C; U-15N]	0.5 mM
18	5'-UUAUUUAUU-3'		0.5 mM
19	Tris-HCl		10 mM
20	KCl		20 mM
21	DTT		2.5 mM
22	ZnSO4		25 uM
23	D2O		100 %

Sample #4

Temperature 298 (±0.5) K, pH 6.2 (±0.1)

#	Name	Isotope labeling	Concentration
24	TIS11d TZF	[U-13C; U-15N]	0.5 mM
25	5'-UUAUUUAUU-3'		0.5 mM
26	TrisHCl		10 mM
27	KCl		20 mM
28	DTT		2.5 mM
29	ZnSO4		25 uM
30	Pf1 filamentous phage		12 mg/mL
31	D2O		100 %

11 CCH-TOCSY

Instrument

#1 Model Bruker Avance (600 MHz)

#2 Model Bruker DMX (750 MHz)

#3 Model Bruker Avance (900 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 6.2 (±0.1)

#	Name	Isotope labeling	Concentration
1	TIS11d TZF	[U-15N]	0.5 mM
2	5'-UUAUUUAUU-3'		0.5 mM
3	TrisHCl		10 mM
4	KCl		20 mM
5	DTT		2.5 mM
6	ZnSO4		25 uM
7	H20		95 %
8	D2O		5 %

Sample #2

Temperature 298 (±0.5) K, pH 6.2 (±0.1)

#	Name	Isotope labeling	Concentration
9	TIS11d TZF	[U-13C; U-15N]	0.5 mM
10	5'-UUAUUUAUU-3'		0.5 mM
11	TrisHCl		10 mM
12	KCl		20 mM
13	DTT		2.5 mM
14	ZnSO4		25 uM
15	H20		95 %
16	D2O		5 %

Sample #3

Temperature 298 (±0.5) K, pH 6.2 (±0.1)

#	Name	Isotope labeling	Concentration
17	TIS11d TZF	[U-13C; U-15N]	0.5 mM
18	5'-UUAUUUAUU-3'		0.5 mM
19	Tris-HCl		10 mM
20	KCl		20 mM
21	DTT		2.5 mM
22	ZnSO4		25 uM
23	D2O		100 %

Sample #4

Temperature 298 (±0.5) K, pH 6.2 (±0.1)

#	Name	Isotope labeling	Concentration
24	TIS11d TZF	[U-13C; U-15N]	0.5 mM
25	5'-UUAUUUAUU-3'		0.5 mM
26	TrisHCl		10 mM
27	KCl		20 mM
28	DTT		2.5 mM
29	ZnSO4		25 uM
30	Pf1 filamentous phage		12 mg/mL
31	D2O		100 %

12 H(C)CH-COSY

Instrument

#1 Model Bruker Avance (600 MHz)

#2 Model Bruker DMX (750 MHz)

#3 Model Bruker Avance (900 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 6.2 (±0.1)

#	Name	Isotope labeling	Concentration
1	TIS11d TZF	[U-15N]	0.5 mM
2	5'-UUAUUUAUU-3'		0.5 mM
3	TrisHCl		10 mM
4	KCl		20 mM
5	DTT		2.5 mM
6	ZnSO4		25 uM
7	H20		95 %
8	D2O		5 %

Sample #2

Temperature 298 (±0.5) K, pH 6.2 (±0.1)

#	Name	Isotope labeling	Concentration
9	TIS11d TZF	[U-13C; U-15N]	0.5 mM
10	5'-UUAUUUAUU-3'		0.5 mM
11	TrisHCl		10 mM
12	KCl		20 mM
13	DTT		2.5 mM
14	ZnSO4		25 uM
15	H20		95 %
16	D2O		5 %

Sample #3

Temperature 298 (±0.5) K, pH 6.2 (±0.1)

#	Name	Isotope labeling	Concentration
17	TIS11d TZF	[U-13C; U-15N]	0.5 mM
18	5'-UUAUUUAUU-3'		0.5 mM
19	Tris-HCl		10 mM
20	KCl		20 mM
21	DTT		2.5 mM
22	ZnSO4		25 uM
23	D2O		100 %

Sample #4

Temperature 298 (±0.5) K, pH 6.2 (±0.1)

#	Name	Isotope labeling	Concentration
24	TIS11d TZF	[U-13C; U-15N]	0.5 mM
25	5'-UUAUUUAUU-3'		0.5 mM
26	TrisHCl		10 mM
27	KCl		20 mM
28	DTT		2.5 mM
29	ZnSO4		25 uM
30	Pf1 filamentous phage		12 mg/mL
31	D2O		100 %

13 H(C)CH-TOCSY

Instrument

#1 Model Bruker Avance (600 MHz)

#2 Model Bruker DMX (750 MHz)

#3 Model Bruker Avance (900 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 6.2 (±0.1)

#	Name	Isotope labeling	Concentration
1	TIS11d TZF	[U-15N]	0.5 mM
2	5'-UUAUUUAUU-3'		0.5 mM
3	TrisHCl		10 mM
4	KCl		20 mM
5	DTT		2.5 mM
6	ZnSO4		25 uM
7	H20		95 %
8	D2O		5 %

Sample #2

Temperature 298 (±0.5) K, pH 6.2 (±0.1)

#	Name	Isotope labeling	Concentration
9	TIS11d TZF	[U-13C; U-15N]	0.5 mM
10	5'-UUAUUUAUU-3'		0.5 mM
11	TrisHCl		10 mM
12	KCl		20 mM
13	DTT		2.5 mM
14	ZnSO4		25 uM
15	H20		95 %
16	D2O		5 %

Sample #3

Temperature 298 (±0.5) K, pH 6.2 (±0.1)

#	Name	Isotope labeling	Concentration
17	TIS11d TZF	[U-13C; U-15N]	0.5 mM
18	5'-UUAUUUAUU-3'		0.5 mM
19	Tris-HCl		10 mM
20	KCl		20 mM
21	DTT		2.5 mM
22	ZnSO4		25 uM
23	D2O		100 %

Sample #4

Temperature 298 (±0.5) K, pH 6.2 (±0.1)

#	Name	Isotope labeling	Concentration
24	TIS11d TZF	[U-13C; U-15N]	0.5 mM
25	5'-UUAUUUAUU-3'		0.5 mM
26	TrisHCl		10 mM
27	KCl		20 mM
28	DTT		2.5 mM
29	ZnSO4		25 uM
30	Pf1 filamentous phage		12 mg/mL
31	D2O		100 %

14 (HB)CB(CGCD)HD

Instrument

#1 Model Bruker Avance (600 MHz)

#2 Model Bruker DMX (750 MHz)

#3 Model Bruker Avance (900 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 6.2 (±0.1)

#	Name	Isotope labeling	Concentration
1	TIS11d TZF	[U-15N]	0.5 mM
2	5'-UUAUUUAUU-3'		0.5 mM
3	TrisHCl		10 mM
4	KCl		20 mM
5	DTT		2.5 mM
6	ZnSO4		25 uM
7	H20		95 %
8	D2O		5 %

Sample #2

Temperature 298 (±0.5) K, pH 6.2 (±0.1)

#	Name	Isotope labeling	Concentration
9	TIS11d TZF	[U-13C; U-15N]	0.5 mM
10	5'-UUAUUUAUU-3'		0.5 mM
11	TrisHCl		10 mM
12	KCl		20 mM
13	DTT		2.5 mM
14	ZnSO4		25 uM
15	H20		95 %
16	D2O		5 %

Sample #3

Temperature 298 (±0.5) K, pH 6.2 (±0.1)

#	Name	Isotope labeling	Concentration
17	TIS11d TZF	[U-13C; U-15N]	0.5 mM
18	5'-UUAUUUAUU-3'		0.5 mM
19	Tris-HCl		10 mM
20	KCl		20 mM
21	DTT		2.5 mM
22	ZnSO4		25 uM
23	D2O		100 %

Sample #4

Temperature 298 (±0.5) K, pH 6.2 (±0.1)

#	Name	Isotope labeling	Concentration
24	TIS11d TZF	[U-13C; U-15N]	0.5 mM
25	5'-UUAUUUAUU-3'		0.5 mM
26	TrisHCl		10 mM
27	KCl		20 mM
28	DTT		2.5 mM
29	ZnSO4		25 uM
30	Pf1 filamentous phage		12 mg/mL
31	D2O		100 %

15 (HB)CB(CGCDCE)HE

Instrument

#1 Model Bruker Avance (600 MHz)

#2 Model Bruker DMX (750 MHz)

#3 Model Bruker Avance (900 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 6.2 (±0.1)

#	Name	Isotope labeling	Concentration
1	TIS11d TZF	[U-15N]	0.5 mM
2	5'-UUAUUUAUU-3'		0.5 mM
3	TrisHCl		10 mM
4	KCl		20 mM
5	DTT		2.5 mM
6	ZnSO4		25 uM
7	H20		95 %
8	D2O		5 %

Sample #2

Temperature 298 (±0.5) K, pH 6.2 (±0.1)

#	Name	Isotope labeling	Concentration
9	TIS11d TZF	[U-13C; U-15N]	0.5 mM
10	5'-UUAUUUAUU-3'		0.5 mM
11	TrisHCl		10 mM
12	KCl		20 mM
13	DTT		2.5 mM
14	ZnSO4		25 uM
15	H20		95 %
16	D2O		5 %

Sample #3

Temperature 298 (±0.5) K, pH 6.2 (±0.1)

#	Name	Isotope labeling	Concentration
17	TIS11d TZF	[U-13C; U-15N]	0.5 mM
18	5'-UUAUUUAUU-3'		0.5 mM
19	Tris-HCl		10 mM
20	KCl		20 mM
21	DTT		2.5 mM
22	ZnSO4		25 uM
23	D2O		100 %

Sample #4

Temperature 298 (±0.5) K, pH 6.2 (±0.1)

#	Name	Isotope labeling	Concentration
24	TIS11d TZF	[U-13C; U-15N]	0.5 mM
25	5'-UUAUUUAUU-3'		0.5 mM
26	TrisHCl		10 mM
27	KCl		20 mM
28	DTT		2.5 mM
29	ZnSO4		25 uM
30	Pf1 filamentous phage		12 mg/mL
31	D2O		100 %

16 3D 15N-separated NOESY

Instrument

#1 Model Bruker Avance (600 MHz)

#2 Model Bruker DMX (750 MHz)

#3 Model Bruker Avance (900 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 6.2 (±0.1)

#	Name	Isotope labeling	Concentration
1	TIS11d TZF	[U-15N]	0.5 mM
2	5'-UUAUUUAUU-3'		0.5 mM
3	TrisHCl		10 mM
4	KCl		20 mM
5	DTT		2.5 mM
6	ZnSO4		25 uM
7	H20		95 %
8	D2O		5 %

Sample #2

Temperature 298 (±0.5) K, pH 6.2 (±0.1)

#	Name	Isotope labeling	Concentration
9	TIS11d TZF	[U-13C; U-15N]	0.5 mM
10	5'-UUAUUUAUU-3'		0.5 mM
11	TrisHCl		10 mM
12	KCl		20 mM
13	DTT		2.5 mM
14	ZnSO4		25 uM
15	H20		95 %
16	D2O		5 %

Sample #3

Temperature 298 (±0.5) K, pH 6.2 (±0.1)

#	Name	Isotope labeling	Concentration
17	TIS11d TZF	[U-13C; U-15N]	0.5 mM
18	5'-UUAUUUAUU-3'		0.5 mM
19	Tris-HCl		10 mM
20	KCl		20 mM
21	DTT		2.5 mM
22	ZnSO4		25 uM
23	D2O		100 %

Sample #4

Temperature 298 (±0.5) K, pH 6.2 (±0.1)

#	Name	Isotope labeling	Concentration
24	TIS11d TZF	[U-13C; U-15N]	0.5 mM
25	5'-UUAUUUAUU-3'		0.5 mM
26	TrisHCl		10 mM
27	KCl		20 mM
28	DTT		2.5 mM
29	ZnSO4		25 uM
30	Pf1 filamentous phage		12 mg/mL
31	D2O		100 %

17 3D 13C-separated NOESY

Instrument

#1 Model Bruker Avance (600 MHz)

#2 Model Bruker DMX (750 MHz)

#3 Model Bruker Avance (900 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 6.2 (±0.1)

#	Name	Isotope labeling	Concentration
1	TIS11d TZF	[U-15N]	0.5 mM
2	5'-UUAUUUAUU-3'		0.5 mM
3	TrisHCl		10 mM
4	KCl		20 mM
5	DTT		2.5 mM
6	ZnSO4		25 uM
7	H20		95 %
8	D2O		5 %

Sample #2

Temperature 298 (±0.5) K, pH 6.2 (±0.1)

#	Name	Isotope labeling	Concentration
9	TIS11d TZF	[U-13C; U-15N]	0.5 mM
10	5'-UUAUUUAUU-3'		0.5 mM
11	TrisHCl		10 mM
12	KCl		20 mM
13	DTT		2.5 mM
14	ZnSO4		25 uM
15	H20		95 %
16	D2O		5 %

Sample #3

Temperature 298 (±0.5) K, pH 6.2 (±0.1)

#	Name	Isotope labeling	Concentration
17	TIS11d TZF	[U-13C; U-15N]	0.5 mM
18	5'-UUAUUUAUU-3'		0.5 mM
19	Tris-HCl		10 mM
20	KCl		20 mM
21	DTT		2.5 mM
22	ZnSO4		25 uM
23	D2O		100 %

Sample #4

Temperature 298 (±0.5) K, pH 6.2 (±0.1)

#	Name	Isotope labeling	Concentration
24	TIS11d TZF	[U-13C; U-15N]	0.5 mM
25	5'-UUAUUUAUU-3'		0.5 mM
26	TrisHCl		10 mM
27	KCl		20 mM
28	DTT		2.5 mM
29	ZnSO4		25 uM
30	Pf1 filamentous phage		12 mg/mL
31	D2O		100 %

18 3D 13C F1-edited, F3-separated NOESY

Instrument

#1 Model Bruker Avance (600 MHz)

#2 Model Bruker DMX (750 MHz)

#3 Model Bruker Avance (900 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 6.2 (±0.1)

#	Name	Isotope labeling	Concentration
1	TIS11d TZF	[U-15N]	0.5 mM
2	5'-UUAUUUAUU-3'		0.5 mM
3	TrisHCl		10 mM
4	KCl		20 mM
5	DTT		2.5 mM
6	ZnSO4		25 uM
7	H20		95 %
8	D2O		5 %

Sample #2

Temperature 298 (±0.5) K, pH 6.2 (±0.1)

#	Name	Isotope labeling	Concentration
9	TIS11d TZF	[U-13C; U-15N]	0.5 mM
10	5'-UUAUUUAUU-3'		0.5 mM
11	TrisHCl		10 mM
12	KCl		20 mM
13	DTT		2.5 mM
14	ZnSO4		25 uM
15	H20		95 %
16	D2O		5 %

Sample #3

Temperature 298 (±0.5) K, pH 6.2 (±0.1)

#	Name	Isotope labeling	Concentration
17	TIS11d TZF	[U-13C; U-15N]	0.5 mM
18	5'-UUAUUUAUU-3'		0.5 mM
19	Tris-HCl		10 mM
20	KCl		20 mM
21	DTT		2.5 mM
22	ZnSO4		25 uM
23	D2O		100 %

Sample #4

Temperature 298 (±0.5) K, pH 6.2 (±0.1)

#	Name	Isotope labeling	Concentration
24	TIS11d TZF	[U-13C; U-15N]	0.5 mM
25	5'-UUAUUUAUU-3'		0.5 mM
26	TrisHCl		10 mM
27	KCl		20 mM
28	DTT		2.5 mM
29	ZnSO4		25 uM
30	Pf1 filamentous phage		12 mg/mL
31	D2O		100 %

19 2D 13C double-half-filtered NOESY

Instrument

#1 Model Bruker Avance (600 MHz)

#2 Model Bruker DMX (750 MHz)

#3 Model Bruker Avance (900 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 6.2 (±0.1)

#	Name	Isotope labeling	Concentration
1	TIS11d TZF	[U-15N]	0.5 mM
2	5'-UUAUUUAUU-3'		0.5 mM
3	TrisHCl		10 mM
4	KCl		20 mM
5	DTT		2.5 mM
6	ZnSO4		25 uM
7	H20		95 %
8	D2O		5 %

Sample #2

Temperature 298 (±0.5) K, pH 6.2 (±0.1)

#	Name	Isotope labeling	Concentration
9	TIS11d TZF	[U-13C; U-15N]	0.5 mM
10	5'-UUAUUUAUU-3'		0.5 mM
11	TrisHCl		10 mM
12	KCl		20 mM
13	DTT		2.5 mM
14	ZnSO4		25 uM
15	H20		95 %
16	D2O		5 %

Sample #3

Temperature 298 (±0.5) K, pH 6.2 (±0.1)

#	Name	Isotope labeling	Concentration
17	TIS11d TZF	[U-13C; U-15N]	0.5 mM
18	5'-UUAUUUAUU-3'		0.5 mM
19	Tris-HCl		10 mM
20	KCl		20 mM
21	DTT		2.5 mM
22	ZnSO4		25 uM
23	D2O		100 %

Sample #4

Temperature 298 (±0.5) K, pH 6.2 (±0.1)

#	Name	Isotope labeling	Concentration
24	TIS11d TZF	[U-13C; U-15N]	0.5 mM
25	5'-UUAUUUAUU-3'		0.5 mM
26	TrisHCl		10 mM
27	KCl		20 mM
28	DTT		2.5 mM
29	ZnSO4		25 uM
30	Pf1 filamentous phage		12 mg/mL
31	D2O		100 %

20 2D 13C double-half-filtered TOCSY

Instrument

#1 Model Bruker Avance (600 MHz)

#2 Model Bruker DMX (750 MHz)

#3 Model Bruker Avance (900 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 6.2 (±0.1)

#	Name	Isotope labeling	Concentration
1	TIS11d TZF	[U-15N]	0.5 mM
2	5'-UUAUUUAUU-3'		0.5 mM
3	TrisHCl		10 mM
4	KCl		20 mM
5	DTT		2.5 mM
6	ZnSO4		25 uM
7	H20		95 %
8	D2O		5 %

Sample #2

Temperature 298 (±0.5) K, pH 6.2 (±0.1)

#	Name	Isotope labeling	Concentration
9	TIS11d TZF	[U-13C; U-15N]	0.5 mM
10	5'-UUAUUUAUU-3'		0.5 mM
11	TrisHCl		10 mM
12	KCl		20 mM
13	DTT		2.5 mM
14	ZnSO4		25 uM
15	H20		95 %
16	D2O		5 %

Sample #3

Temperature 298 (±0.5) K, pH 6.2 (±0.1)

#	Name	Isotope labeling	Concentration
17	TIS11d TZF	[U-13C; U-15N]	0.5 mM
18	5'-UUAUUUAUU-3'		0.5 mM
19	Tris-HCl		10 mM
20	KCl		20 mM
21	DTT		2.5 mM
22	ZnSO4		25 uM
23	D2O		100 %

Sample #4

Temperature 298 (±0.5) K, pH 6.2 (±0.1)

#	Name	Isotope labeling	Concentration
24	TIS11d TZF	[U-13C; U-15N]	0.5 mM
25	5'-UUAUUUAUU-3'		0.5 mM
26	TrisHCl		10 mM
27	KCl		20 mM
28	DTT		2.5 mM
29	ZnSO4		25 uM
30	Pf1 filamentous phage		12 mg/mL
31	D2O		100 %

21 HNHA

Instrument

#1 Model Bruker Avance (600 MHz)

#2 Model Bruker DMX (750 MHz)

#3 Model Bruker Avance (900 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 6.2 (±0.1)

#	Name	Isotope labeling	Concentration
1	TIS11d TZF	[U-15N]	0.5 mM
2	5'-UUAUUUAUU-3'		0.5 mM
3	TrisHCl		10 mM
4	KCl		20 mM
5	DTT		2.5 mM
6	ZnSO4		25 uM
7	H20		95 %
8	D2O		5 %

Sample #2

Temperature 298 (±0.5) K, pH 6.2 (±0.1)

#	Name	Isotope labeling	Concentration
9	TIS11d TZF	[U-13C; U-15N]	0.5 mM
10	5'-UUAUUUAUU-3'		0.5 mM
11	TrisHCl		10 mM
12	KCl		20 mM
13	DTT		2.5 mM
14	ZnSO4		25 uM
15	H20		95 %
16	D2O		5 %

Sample #3

Temperature 298 (±0.5) K, pH 6.2 (±0.1)

#	Name	Isotope labeling	Concentration
17	TIS11d TZF	[U-13C; U-15N]	0.5 mM
18	5'-UUAUUUAUU-3'		0.5 mM
19	Tris-HCl		10 mM
20	KCl		20 mM
21	DTT		2.5 mM
22	ZnSO4		25 uM
23	D2O		100 %

Sample #4

Temperature 298 (±0.5) K, pH 6.2 (±0.1)

#	Name	Isotope labeling	Concentration
24	TIS11d TZF	[U-13C; U-15N]	0.5 mM
25	5'-UUAUUUAUU-3'		0.5 mM
26	TrisHCl		10 mM
27	KCl		20 mM
28	DTT		2.5 mM
29	ZnSO4		25 uM
30	Pf1 filamentous phage		12 mg/mL
31	D2O		100 %

22 HNHB

Instrument

#1 Model Bruker Avance (600 MHz)

#2 Model Bruker DMX (750 MHz)

#3 Model Bruker Avance (900 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 6.2 (±0.1)

#	Name	Isotope labeling	Concentration
1	TIS11d TZF	[U-15N]	0.5 mM
2	5'-UUAUUUAUU-3'		0.5 mM
3	TrisHCl		10 mM
4	KCl		20 mM
5	DTT		2.5 mM
6	ZnSO4		25 uM
7	H20		95 %
8	D2O		5 %

Sample #2

Temperature 298 (±0.5) K, pH 6.2 (±0.1)

#	Name	Isotope labeling	Concentration
9	TIS11d TZF	[U-13C; U-15N]	0.5 mM
10	5'-UUAUUUAUU-3'		0.5 mM
11	TrisHCl		10 mM
12	KCl		20 mM
13	DTT		2.5 mM
14	ZnSO4		25 uM
15	H20		95 %
16	D2O		5 %

Sample #3

Temperature 298 (±0.5) K, pH 6.2 (±0.1)

#	Name	Isotope labeling	Concentration
17	TIS11d TZF	[U-13C; U-15N]	0.5 mM
18	5'-UUAUUUAUU-3'		0.5 mM
19	Tris-HCl		10 mM
20	KCl		20 mM
21	DTT		2.5 mM
22	ZnSO4		25 uM
23	D2O		100 %

Sample #4

Temperature 298 (±0.5) K, pH 6.2 (±0.1)

#	Name	Isotope labeling	Concentration
24	TIS11d TZF	[U-13C; U-15N]	0.5 mM
25	5'-UUAUUUAUU-3'		0.5 mM
26	TrisHCl		10 mM
27	KCl		20 mM
28	DTT		2.5 mM
29	ZnSO4		25 uM
30	Pf1 filamentous phage		12 mg/mL
31	D2O		100 %

23 HACAHB-COSY

Instrument

#1 Model Bruker Avance (600 MHz)

#2 Model Bruker DMX (750 MHz)

#3 Model Bruker Avance (900 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 6.2 (±0.1)

#	Name	Isotope labeling	Concentration
1	TIS11d TZF	[U-15N]	0.5 mM
2	5'-UUAUUUAUU-3'		0.5 mM
3	TrisHCl		10 mM
4	KCl		20 mM
5	DTT		2.5 mM
6	ZnSO4		25 uM
7	H20		95 %
8	D2O		5 %

Sample #2

Temperature 298 (±0.5) K, pH 6.2 (±0.1)

#	Name	Isotope labeling	Concentration
9	TIS11d TZF	[U-13C; U-15N]	0.5 mM
10	5'-UUAUUUAUU-3'		0.5 mM
11	TrisHCl		10 mM
12	KCl		20 mM
13	DTT		2.5 mM
14	ZnSO4		25 uM
15	H20		95 %
16	D2O		5 %

Sample #3

Temperature 298 (±0.5) K, pH 6.2 (±0.1)

#	Name	Isotope labeling	Concentration
17	TIS11d TZF	[U-13C; U-15N]	0.5 mM
18	5'-UUAUUUAUU-3'		0.5 mM
19	Tris-HCl		10 mM
20	KCl		20 mM
21	DTT		2.5 mM
22	ZnSO4		25 uM
23	D2O		100 %

Sample #4

Temperature 298 (±0.5) K, pH 6.2 (±0.1)

#	Name	Isotope labeling	Concentration
24	TIS11d TZF	[U-13C; U-15N]	0.5 mM
25	5'-UUAUUUAUU-3'		0.5 mM
26	TrisHCl		10 mM
27	KCl		20 mM
28	DTT		2.5 mM
29	ZnSO4		25 uM
30	Pf1 filamentous phage		12 mg/mL
31	D2O		100 %

24 13C-{13CO} spin-echo difference CT-HSQC

Instrument

#1 Model Bruker Avance (600 MHz)

#2 Model Bruker DMX (750 MHz)

#3 Model Bruker Avance (900 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 6.2 (±0.1)

#	Name	Isotope labeling	Concentration
1	TIS11d TZF	[U-15N]	0.5 mM
2	5'-UUAUUUAUU-3'		0.5 mM
3	TrisHCl		10 mM
4	KCl		20 mM
5	DTT		2.5 mM
6	ZnSO4		25 uM
7	H20		95 %
8	D2O		5 %

Sample #2

Temperature 298 (±0.5) K, pH 6.2 (±0.1)

#	Name	Isotope labeling	Concentration
9	TIS11d TZF	[U-13C; U-15N]	0.5 mM
10	5'-UUAUUUAUU-3'		0.5 mM
11	TrisHCl		10 mM
12	KCl		20 mM
13	DTT		2.5 mM
14	ZnSO4		25 uM
15	H20		95 %
16	D2O		5 %

Sample #3

Temperature 298 (±0.5) K, pH 6.2 (±0.1)

#	Name	Isotope labeling	Concentration
17	TIS11d TZF	[U-13C; U-15N]	0.5 mM
18	5'-UUAUUUAUU-3'		0.5 mM
19	Tris-HCl		10 mM
20	KCl		20 mM
21	DTT		2.5 mM
22	ZnSO4		25 uM
23	D2O		100 %

Sample #4

Temperature 298 (±0.5) K, pH 6.2 (±0.1)

#	Name	Isotope labeling	Concentration
24	TIS11d TZF	[U-13C; U-15N]	0.5 mM
25	5'-UUAUUUAUU-3'		0.5 mM
26	TrisHCl		10 mM
27	KCl		20 mM
28	DTT		2.5 mM
29	ZnSO4		25 uM
30	Pf1 filamentous phage		12 mg/mL
31	D2O		100 %

25 13C-{15N} spin-echo difference CT-HSQC

Instrument

#1 Model Bruker Avance (600 MHz)

#2 Model Bruker DMX (750 MHz)

#3 Model Bruker Avance (900 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 6.2 (±0.1)

#	Name	Isotope labeling	Concentration
1	TIS11d TZF	[U-15N]	0.5 mM
2	5'-UUAUUUAUU-3'		0.5 mM
3	TrisHCl		10 mM
4	KCl		20 mM
5	DTT		2.5 mM
6	ZnSO4		25 uM
7	H20		95 %
8	D2O		5 %

Sample #2

Temperature 298 (±0.5) K, pH 6.2 (±0.1)

#	Name	Isotope labeling	Concentration
9	TIS11d TZF	[U-13C; U-15N]	0.5 mM
10	5'-UUAUUUAUU-3'		0.5 mM
11	TrisHCl		10 mM
12	KCl		20 mM
13	DTT		2.5 mM
14	ZnSO4		25 uM
15	H20		95 %
16	D2O		5 %

Sample #3

Temperature 298 (±0.5) K, pH 6.2 (±0.1)

#	Name	Isotope labeling	Concentration
17	TIS11d TZF	[U-13C; U-15N]	0.5 mM
18	5'-UUAUUUAUU-3'		0.5 mM
19	Tris-HCl		10 mM
20	KCl		20 mM
21	DTT		2.5 mM
22	ZnSO4		25 uM
23	D2O		100 %

Sample #4

Temperature 298 (±0.5) K, pH 6.2 (±0.1)

#	Name	Isotope labeling	Concentration
24	TIS11d TZF	[U-13C; U-15N]	0.5 mM
25	5'-UUAUUUAUU-3'		0.5 mM
26	TrisHCl		10 mM
27	KCl		20 mM
28	DTT		2.5 mM
29	ZnSO4		25 uM
30	Pf1 filamentous phage		12 mg/mL
31	D2O		100 %

26 IPAP-[1H,15N]-HSQC

Instrument

#1 Model Bruker Avance (600 MHz)

#2 Model Bruker DMX (750 MHz)

#3 Model Bruker Avance (900 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 6.2 (±0.1)

#	Name	Isotope labeling	Concentration
1	TIS11d TZF	[U-15N]	0.5 mM
2	5'-UUAUUUAUU-3'		0.5 mM
3	TrisHCl		10 mM
4	KCl		20 mM
5	DTT		2.5 mM
6	ZnSO4		25 uM
7	H20		95 %
8	D2O		5 %

Sample #2

Temperature 298 (±0.5) K, pH 6.2 (±0.1)

#	Name	Isotope labeling	Concentration
9	TIS11d TZF	[U-13C; U-15N]	0.5 mM
10	5'-UUAUUUAUU-3'		0.5 mM
11	TrisHCl		10 mM
12	KCl		20 mM
13	DTT		2.5 mM
14	ZnSO4		25 uM
15	H20		95 %
16	D2O		5 %

Sample #3

Temperature 298 (±0.5) K, pH 6.2 (±0.1)

#	Name	Isotope labeling	Concentration
17	TIS11d TZF	[U-13C; U-15N]	0.5 mM
18	5'-UUAUUUAUU-3'		0.5 mM
19	Tris-HCl		10 mM
20	KCl		20 mM
21	DTT		2.5 mM
22	ZnSO4		25 uM
23	D2O		100 %

Sample #4

Temperature 298 (±0.5) K, pH 6.2 (±0.1)

#	Name	Isotope labeling	Concentration
24	TIS11d TZF	[U-13C; U-15N]	0.5 mM
25	5'-UUAUUUAUU-3'		0.5 mM
26	TrisHCl		10 mM
27	KCl		20 mM
28	DTT		2.5 mM
29	ZnSO4		25 uM
30	Pf1 filamentous phage		12 mg/mL
31	D2O		100 %

27 IPAP-HNCO

Instrument

#1 Model Bruker Avance (600 MHz)

#2 Model Bruker DMX (750 MHz)

#3 Model Bruker Avance (900 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 6.2 (±0.1)

#	Name	Isotope labeling	Concentration
1	TIS11d TZF	[U-15N]	0.5 mM
2	5'-UUAUUUAUU-3'		0.5 mM
3	TrisHCl		10 mM
4	KCl		20 mM
5	DTT		2.5 mM
6	ZnSO4		25 uM
7	H20		95 %
8	D2O		5 %

Sample #2

Temperature 298 (±0.5) K, pH 6.2 (±0.1)

#	Name	Isotope labeling	Concentration
9	TIS11d TZF	[U-13C; U-15N]	0.5 mM
10	5'-UUAUUUAUU-3'		0.5 mM
11	TrisHCl		10 mM
12	KCl		20 mM
13	DTT		2.5 mM
14	ZnSO4		25 uM
15	H20		95 %
16	D2O		5 %

Sample #3

Temperature 298 (±0.5) K, pH 6.2 (±0.1)

#	Name	Isotope labeling	Concentration
17	TIS11d TZF	[U-13C; U-15N]	0.5 mM
18	5'-UUAUUUAUU-3'		0.5 mM
19	Tris-HCl		10 mM
20	KCl		20 mM
21	DTT		2.5 mM
22	ZnSO4		25 uM
23	D2O		100 %

Sample #4

Temperature 298 (±0.5) K, pH 6.2 (±0.1)

#	Name	Isotope labeling	Concentration
24	TIS11d TZF	[U-13C; U-15N]	0.5 mM
25	5'-UUAUUUAUU-3'		0.5 mM
26	TrisHCl		10 mM
27	KCl		20 mM
28	DTT		2.5 mM
29	ZnSO4		25 uM
30	Pf1 filamentous phage		12 mg/mL
31	D2O		100 %

nullKeywords butyrate, complex, degradation ARE UTR Nup475, ERF, intercalate, intercalation, response factor, single-stranded, TIS11, TTP tristetraprolin RNA

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BMRB: 6005

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Entries sharing articles Swiss-Prot: 1 entries Detail

Related entities 1. TIS11d TZF, : 1 : 6 : 164 entities Detail

Experiments performed 27 experiments Detail

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