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BMRB: 6007

1RJH

1H, 13C and 15N resonance assignment of the Plasminogen Kringle 4 Binding Calcium Free Form of the C-type Lectin-like Domain of Tetranectin

Authors

Nielbo, S., Thomsen, J.K., Jensen, P.H., Graversen, J.H., Etzerodt, M., Poulsen, F.M., Thogersen, H.C.

Assembly

tn3 calcium free form

Entity

1. tn3 calcium free form (polymer, Thiol state: all disulfide bound), 137 monomers, 15121.86 Da Detail

ALQTVCLKGT KVHMKCFLAF TQTKTFHEAS EDCISRGGTL STPQTGSEND ALYEYLRQSV GNEAEIWLGL NDMAAEGTWV DMTGARIAYK NWETEITAQP DGGKTENCAV LSGAANGKWF DKRCRDQLPY ICQFGIV

Formula weight

15121.86 Da

Entity Connection

disulfide 3 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS6:SG	1:CYS16:SG
2	disulfide	sing	1:CYS33:SG	1:CYS132:SG
3	disulfide	sing	1:CYS108:SG	1:CYS124:SG

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

simulated annealing protocol with an explicit layer of water

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 86.9 %, Completeness: 74.1 %, Completeness (bb): 80.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	74.1 % (1146 of 1546)	71.7 % (571 of 796)	76.8 % (460 of 599)	76.2 % (115 of 151)
Backbone	80.3 % (655 of 816)	81.3 % (231 of 284)	79.9 % (318 of 398)	79.1 % (106 of 134)
Sidechain	69.6 % (594 of 854)	66.4 % (340 of 512)	75.4 % (245 of 325)	52.9 % (9 of 17)
Aromatic	63.0 % (87 of 138)	62.3 % (43 of 69)	61.5 % (40 of 65)	100.0 % (4 of 4)
Methyl	80.0 % (112 of 140)	80.0 % (56 of 70)	80.0 % (56 of 70)

1. CTLD of tetranectin

ALQTVCLKGT KVHMKCFLAF TQTKTFHEAS EDCISRGGTL STPQTGSEND ALYEYLRQSV GNEAEIWLGL NDMAAEGTWV DMTGARIAYK NWETEITAQP DGGKTENCAV LSGAANGKWF DKRCRDQLPY ICQFGIV

Show sample condition

Sample

Temperature 298 (±1) K, pH 6.7 (±0.1)

#	Name	Isotope labeling	Concentration
1	CTLD of tetranectin	[U-13C; U-15N]	0.6 mM
2	EDTA		2 mM
3	NaN3		1 mM
4	D2O		10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.23 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.23 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.0 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.09 ppm, Outliers: 3 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 1RJH, Strand ID: A Detail

Release date

2003-11-13

Citation

Structure of the plasminogen kringle 4 binding calcium-free form of the C-type lectin-like domain of tetranectin
Nielbo, S., Thomsen, J.K., Jensen, P.H., Graversen, J.H., Etzerodt, M., Poulsen, F.M., Thogersen, H.C.
Biochemistry (2004), 43, 8636-8643, PubMed 15236571 , DOI 10.1021/bi049570s , Abstract

Related entities 1. tn3 calcium free form, : 1 : 2 : 2 : 364 entities Detail

Interaction partners 1. tn3 calcium free form, : 13 interactors Detail

More details for 13 interactors identified by 2 citations

Experiments performed 11 experiments Detail

1 15N-HSQC