BMRBj - BMREntryMaster

Found 1 Document (1.413 seconds)

BMRB: 6009

1RDE

NMR structure of the thrombin-binding DNA aptamer stabilized by Sr2+

Authors

Mao, X., Marky, L.A., Gmeiner, W.H.

Assembly

Thrombin-binding DNA aptamer

Entity

1. thrombin binding 15mer DNA aptamer (polymer, Thiol state: not present), 15 monomers, 4806.023 Da Detail

GGTTGGTGTG GTTGG

2. SR (non-polymer), 87.620 Da

Total weight

4893.643 Da

Max. entity weight

4806.023 Da

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 93.3 %, Completeness: 93.3 %, Completeness (bb): 93.3 % Detail

Polymer type: polydeoxyribonucleotide

	Total	³¹P
All	93.3 % (14 of 15)	93.3 % (14 of 15)
Suger, PO4	93.3 % (14 of 15)	93.3 % (14 of 15)

1. thrombin binding 15mer DNA aptamer

GGTTGGTGTG GTTGG

Show sample condition

Sample #1

Pressure 1 atm, Temperature 288 (±0.5) K, pH 4.7 (±0.1)

#	Name	Concentration
1	thrombin binding 15mer DNA aptamer	1 mM
2	SRONTIUM (II) ION	1.0 ~ 1.3 mM
3	D2O	100 %

Sample #2

Pressure 1 atm, Temperature 288 (±0.5) K, pH 4.7 (±0.1)

#	Name	Concentration
4	thrombin binding 15mer DNA aptamer	1 mM
5	SRONTIUM (II) ION	1.0 ~ 1.3 mM
6	H2O	90 %
7	D2O	10 %

Release date

2004-08-29

Citation

NMR structure of the thrombin-binding DNA aptamer stabilized by Sr2+
Mao, X., Marky, L.A., Gmeiner, W.H.
J. Biomol. Struct. Dyn. (2004), 22, 25-33, PubMed 15214802 , DOI 10.1080/07391102.2004.10506977 , Abstract

Experiments performed 5 experiments Detail

1 2D NOESY

Instrument

Model Bruker AVANCE (600 MHz)

Sample #1

Pressure 1 atm, Temperature 288 (±0.5) K, pH 4.7 (±0.1)

#	Name	Concentration
1	thrombin binding 15mer DNA aptamer	1 mM
2	SRONTIUM (II) ION	1.0 ~ 1.3 mM
3	D2O	100 %

Sample #2

Pressure 1 atm, Temperature 288 (±0.5) K, pH 4.7 (±0.1)

#	Name	Concentration
4	thrombin binding 15mer DNA aptamer	1 mM
5	SRONTIUM (II) ION	1.0 ~ 1.3 mM
6	H2O	90 %
7	D2O	10 %

2 2D TOCSY

Instrument

Model Bruker AVANCE (600 MHz)

Sample #1

Pressure 1 atm, Temperature 288 (±0.5) K, pH 4.7 (±0.1)

#	Name	Concentration
1	thrombin binding 15mer DNA aptamer	1 mM
2	SRONTIUM (II) ION	1.0 ~ 1.3 mM
3	D2O	100 %

Sample #2

Pressure 1 atm, Temperature 288 (±0.5) K, pH 4.7 (±0.1)

#	Name	Concentration
4	thrombin binding 15mer DNA aptamer	1 mM
5	SRONTIUM (II) ION	1.0 ~ 1.3 mM
6	H2O	90 %
7	D2O	10 %

3 E-COSY

Instrument

Model Bruker AVANCE (600 MHz)

Sample #1

Pressure 1 atm, Temperature 288 (±0.5) K, pH 4.7 (±0.1)

#	Name	Concentration
1	thrombin binding 15mer DNA aptamer	1 mM
2	SRONTIUM (II) ION	1.0 ~ 1.3 mM
3	D2O	100 %

Sample #2

Pressure 1 atm, Temperature 288 (±0.5) K, pH 4.7 (±0.1)

#	Name	Concentration
4	thrombin binding 15mer DNA aptamer	1 mM
5	SRONTIUM (II) ION	1.0 ~ 1.3 mM
6	H2O	90 %
7	D2O	10 %

4 P31-H1 COSY

Instrument

Model Bruker AVANCE (600 MHz)

Sample #1

Pressure 1 atm, Temperature 288 (±0.5) K, pH 4.7 (±0.1)

#	Name	Concentration
1	thrombin binding 15mer DNA aptamer	1 mM
2	SRONTIUM (II) ION	1.0 ~ 1.3 mM
3	D2O	100 %

Sample #2

Pressure 1 atm, Temperature 288 (±0.5) K, pH 4.7 (±0.1)

#	Name	Concentration
4	thrombin binding 15mer DNA aptamer	1 mM
5	SRONTIUM (II) ION	1.0 ~ 1.3 mM
6	H2O	90 %
7	D2O	10 %

5 ROESY

Instrument

Model Bruker AVANCE (600 MHz)

Sample #1

Pressure 1 atm, Temperature 288 (±0.5) K, pH 4.7 (±0.1)

#	Name	Concentration
1	thrombin binding 15mer DNA aptamer	1 mM
2	SRONTIUM (II) ION	1.0 ~ 1.3 mM
3	D2O	100 %

Sample #2

Pressure 1 atm, Temperature 288 (±0.5) K, pH 4.7 (±0.1)

#	Name	Concentration
4	thrombin binding 15mer DNA aptamer	1 mM
5	SRONTIUM (II) ION	1.0 ~ 1.3 mM
6	H2O	90 %
7	D2O	10 %

nullKeywords quadruplex, Sr2+, thrombin-binding DNA

Search

Query history

Resources

Search for primary databases

Search for subsidiary databases

Display

Hits per page

Statistics

Query Changes will take effect after "OK" button is selected.

Maximum hits

Default conjunction operator

Omni-box

Search filter

Sequence search (BLAST)

E-value

Query sequence coverage

Miscellaneous

The number of previewed publications

Completeness of assigned chemical shift

Sequence chart about top aligned regions

Sequence chart about interacting regions

Hit context with highlight

Link destinations for BMRB, PDB and EMDB

Found 1 Document (1.413 seconds)

BMRB: 6009

Sample #1

Sample #2

Experiments performed 5 experiments Detail

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2