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Found 1 Document (7.816 seconds)

BMRB: 6009

1RDE

NMR structure of the thrombin-binding DNA aptamer stabilized by Sr2+

Authors

Mao, X., Marky, L.A., Gmeiner, W.H.

Assembly

Thrombin-binding DNA aptamer

Entity

1. thrombin binding 15mer DNA aptamer (polymer, Thiol state: not present), 15 monomers, 4806.023 Da Detail

GGTTGGTGTG GTTGG

2. SR (non-polymer), 87.620 Da

Total weight

4893.643 Da

Max. entity weight

4806.023 Da

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 93.3 %, Completeness: 93.3 %, Completeness (bb): 93.3 % Detail

Polymer type: polydeoxyribonucleotide

	Total	³¹P
All	93.3 % (14 of 15)	93.3 % (14 of 15)
Suger, PO4	93.3 % (14 of 15)	93.3 % (14 of 15)

1. thrombin binding 15mer DNA aptamer

GGTTGGTGTG GTTGG

Show sample condition

Sample #1

Pressure 1 atm, Temperature 288 (±0.5) K, pH 4.7 (±0.1)

#	Name	Concentration
1	thrombin binding 15mer DNA aptamer	1 mM
2	SRONTIUM (II) ION	1.0 ~ 1.3 mM
3	D2O	100 %

Sample #2

Pressure 1 atm, Temperature 288 (±0.5) K, pH 4.7 (±0.1)

#	Name	Concentration
4	thrombin binding 15mer DNA aptamer	1 mM
5	SRONTIUM (II) ION	1.0 ~ 1.3 mM
6	H2O	90 %
7	D2O	10 %

Release date

2004-08-29

Citation

NMR structure of the thrombin-binding DNA aptamer stabilized by Sr2+
Mao, X., Marky, L.A., Gmeiner, W.H.
J. Biomol. Struct. Dyn. (2004), 22, 25-33, PubMed 15214802 , DOI 10.1080/07391102.2004.10506977 , Abstract

Experiments performed 5 experiments Detail

1 2D NOESY

Instrument

Model Bruker AVANCE (600 MHz)

Sample #1

Pressure 1 atm, Temperature 288 (±0.5) K, pH 4.7 (±0.1)

#	Name	Concentration
1	thrombin binding 15mer DNA aptamer	1 mM
2	SRONTIUM (II) ION	1.0 ~ 1.3 mM
3	D2O	100 %

Sample #2

Pressure 1 atm, Temperature 288 (±0.5) K, pH 4.7 (±0.1)

#	Name	Concentration
4	thrombin binding 15mer DNA aptamer	1 mM
5	SRONTIUM (II) ION	1.0 ~ 1.3 mM
6	H2O	90 %
7	D2O	10 %

2 2D TOCSY

Instrument

Model Bruker AVANCE (600 MHz)

Sample #1

Pressure 1 atm, Temperature 288 (±0.5) K, pH 4.7 (±0.1)

#	Name	Concentration
1	thrombin binding 15mer DNA aptamer	1 mM
2	SRONTIUM (II) ION	1.0 ~ 1.3 mM
3	D2O	100 %

Sample #2

Pressure 1 atm, Temperature 288 (±0.5) K, pH 4.7 (±0.1)

#	Name	Concentration
4	thrombin binding 15mer DNA aptamer	1 mM
5	SRONTIUM (II) ION	1.0 ~ 1.3 mM
6	H2O	90 %
7	D2O	10 %

3 E-COSY

Instrument

Model Bruker AVANCE (600 MHz)

Sample #1

Pressure 1 atm, Temperature 288 (±0.5) K, pH 4.7 (±0.1)

#	Name	Concentration
1	thrombin binding 15mer DNA aptamer	1 mM
2	SRONTIUM (II) ION	1.0 ~ 1.3 mM
3	D2O	100 %

Sample #2

Pressure 1 atm, Temperature 288 (±0.5) K, pH 4.7 (±0.1)

#	Name	Concentration
4	thrombin binding 15mer DNA aptamer	1 mM
5	SRONTIUM (II) ION	1.0 ~ 1.3 mM
6	H2O	90 %
7	D2O	10 %

4 P31-H1 COSY

Instrument

Model Bruker AVANCE (600 MHz)

Sample #1

Pressure 1 atm, Temperature 288 (±0.5) K, pH 4.7 (±0.1)

#	Name	Concentration
1	thrombin binding 15mer DNA aptamer	1 mM
2	SRONTIUM (II) ION	1.0 ~ 1.3 mM
3	D2O	100 %

Sample #2

Pressure 1 atm, Temperature 288 (±0.5) K, pH 4.7 (±0.1)

#	Name	Concentration
4	thrombin binding 15mer DNA aptamer	1 mM
5	SRONTIUM (II) ION	1.0 ~ 1.3 mM
6	H2O	90 %
7	D2O	10 %

5 ROESY

Instrument

Model Bruker AVANCE (600 MHz)

Sample #1

Pressure 1 atm, Temperature 288 (±0.5) K, pH 4.7 (±0.1)

#	Name	Concentration
1	thrombin binding 15mer DNA aptamer	1 mM
2	SRONTIUM (II) ION	1.0 ~ 1.3 mM
3	D2O	100 %

Sample #2

Pressure 1 atm, Temperature 288 (±0.5) K, pH 4.7 (±0.1)

#	Name	Concentration
4	thrombin binding 15mer DNA aptamer	1 mM
5	SRONTIUM (II) ION	1.0 ~ 1.3 mM
6	H2O	90 %
7	D2O	10 %

nullKeywords quadruplex, Sr2+, thrombin-binding DNA

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Found 1 Document (7.816 seconds)

BMRB: 6009

Sample #1

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Experiments performed 5 experiments Detail

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