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Found 1 Document (1.459 seconds)

BMRB: 6010

1RHX

High-Resolution NMR Structure of the Conserved Hypothetical Protein TM0979 from Thermotoga maritima

Authors

Peti, W., Herrmann, T., Wuthrich, K.

Assembly

tm0979

Entity

1. tm0979 (polymer, Thiol state: not present), 87 monomers, 9876.241 Da Detail

MALVLVKYGT DHPVEKLKIR SAKAEDKIVL IQNGVFWALE ELETPAKVYA IKDDFLARGY SEEDSKVPLI TYSEFIDLLE GEEKFIG

Formula weight

9876.241 Da

Source organism

Thermotoga maritima

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.9 %, Completeness: 89.1 %, Completeness (bb): 97.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	89.1 % (933 of 1047)	88.4 % (479 of 542)	88.3 % (369 of 418)	97.7 % (85 of 87)
Backbone	97.7 % (504 of 516)	98.3 % (173 of 176)	97.3 % (249 of 256)	97.6 % (82 of 84)
Sidechain	83.2 % (510 of 613)	83.6 % (306 of 366)	82.4 % (201 of 244)	100.0 % (3 of 3)
Aromatic	40.9 % (36 of 88)	70.5 % (31 of 44)	9.3 % (4 of 43)	100.0 % (1 of 1)
Methyl	100.0 % (116 of 116)	100.0 % (58 of 58)	100.0 % (58 of 58)

1. TM0979

MALVLVKYGT DHPVEKLKIR SAKAEDKIVL IQNGVFWALE ELETPAKVYA IKDDFLARGY SEEDSKVPLI TYSEFIDLLE GEEKFIG

Show sample condition

Sample #1

Temperature 313 (±0.2) K

#	Name	Isotope labeling	Type	Concentration
1	TM0979	[U-13C; U-15N]		2.0 ~ 10.0 mM

Sample #2

Temperature 313 (±0.2) K

#	Name	Isotope labeling	Type	Concentration
2	TM0979	[U-15N]		2.0 ~ 10.0 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.15 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.15 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.13 ppm, Outliers: 3 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.44 ppm, Outliers: 5 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 1RHX, Strand ID: A Detail

Release date

2005-02-28

Citation

NMR structure of the conserved hypothetical protein TM0979 from Thermotoga maritima
Peti, W., Herrmann, T., Zagnitko, O., Grzechnik, S.K., Wuthrich, K.
Proteins (2005), 59, 387-390, PubMed 15723348 , DOI 10.1002/prot.20352 , Abstract

Related entities 1. tm0979, : 1 : 1 : 1 entities Detail

Experiments performed 8 experiments Detail

1 15N HSQC

Instrument

#1 Model Bruker Avance (600 MHz)

#2 Model Bruker DRX (800 MHz)

Sample #1

Temperature 313 (±0.2) K

#	Name	Isotope labeling	Type	Concentration
1	TM0979	[U-13C; U-15N]		2.0 ~ 10.0 mM

Sample #2

Temperature 313 (±0.2) K

#	Name	Isotope labeling	Type	Concentration
2	TM0979	[U-15N]		2.0 ~ 10.0 mM

2 HNCACB

Instrument

#1 Model Bruker Avance (600 MHz)

#2 Model Bruker DRX (800 MHz)

Sample #1

Temperature 313 (±0.2) K

#	Name	Isotope labeling	Type	Concentration
1	TM0979	[U-13C; U-15N]		2.0 ~ 10.0 mM

Sample #2

Temperature 313 (±0.2) K

#	Name	Isotope labeling	Type	Concentration
2	TM0979	[U-15N]		2.0 ~ 10.0 mM

3 CBCACONH

Instrument

#1 Model Bruker Avance (600 MHz)

#2 Model Bruker DRX (800 MHz)

Sample #1

Temperature 313 (±0.2) K

#	Name	Isotope labeling	Type	Concentration
1	TM0979	[U-13C; U-15N]		2.0 ~ 10.0 mM

Sample #2

Temperature 313 (±0.2) K

#	Name	Isotope labeling	Type	Concentration
2	TM0979	[U-15N]		2.0 ~ 10.0 mM

4 HCCH-TOCSY

Instrument

#1 Model Bruker Avance (600 MHz)

#2 Model Bruker DRX (800 MHz)

Sample #1

Temperature 313 (±0.2) K

#	Name	Isotope labeling	Type	Concentration
1	TM0979	[U-13C; U-15N]		2.0 ~ 10.0 mM

Sample #2

Temperature 313 (±0.2) K

#	Name	Isotope labeling	Type	Concentration
2	TM0979	[U-15N]		2.0 ~ 10.0 mM

5 HBHACONH

Instrument

#1 Model Bruker Avance (600 MHz)

#2 Model Bruker DRX (800 MHz)

Sample #1

Temperature 313 (±0.2) K

#	Name	Isotope labeling	Type	Concentration
1	TM0979	[U-13C; U-15N]		2.0 ~ 10.0 mM

Sample #2

Temperature 313 (±0.2) K

#	Name	Isotope labeling	Type	Concentration
2	TM0979	[U-15N]		2.0 ~ 10.0 mM

6 HNCO

Instrument

#1 Model Bruker Avance (600 MHz)

#2 Model Bruker DRX (800 MHz)

Sample #1

Temperature 313 (±0.2) K

#	Name	Isotope labeling	Type	Concentration
1	TM0979	[U-13C; U-15N]		2.0 ~ 10.0 mM

Sample #2

Temperature 313 (±0.2) K

#	Name	Isotope labeling	Type	Concentration
2	TM0979	[U-15N]		2.0 ~ 10.0 mM

7 15N NOESY

Instrument

#1 Model Bruker Avance (600 MHz)

#2 Model Bruker DRX (800 MHz)

Sample #1

Temperature 313 (±0.2) K

#	Name	Isotope labeling	Type	Concentration
1	TM0979	[U-13C; U-15N]		2.0 ~ 10.0 mM

Sample #2

Temperature 313 (±0.2) K

#	Name	Isotope labeling	Type	Concentration
2	TM0979	[U-15N]		2.0 ~ 10.0 mM

8 13C NOESY

Instrument

#1 Model Bruker Avance (600 MHz)

#2 Model Bruker DRX (800 MHz)

Sample #1

Temperature 313 (±0.2) K

#	Name	Isotope labeling	Type	Concentration
1	TM0979	[U-13C; U-15N]		2.0 ~ 10.0 mM

Sample #2

Temperature 313 (±0.2) K

#	Name	Isotope labeling	Type	Concentration
2	TM0979	[U-15N]		2.0 ~ 10.0 mM

nullKeywords NMR, structure, JCSG, TM0979

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Found 1 Document (1.459 seconds)

BMRB: 6010

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Chemical shift reference

Related entities 1. tm0979, : 1 : 1 : 1 entities Detail

Experiments performed 8 experiments Detail

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