BMRBj - BMREntryMaster

Found 1 Document (1.938 seconds)

BMRB: 6011

1RJJ

Solution structure of a homodimeric hypothetical protein, At5g22580, a structural genomics target from Arabidopsis Thaliana

Authors

Cornilescu, G., Cornilescu, C.C., Zhao, Q., Frederick, R.O., Peterson, F.C., Thao, S., Markley, J.L.

Assembly

At5g22580

Entity

1. At5g22580 (polymer, Thiol state: not present), 111 monomers, 12348.95 × 2 Da Detail

MATSGFKHLV VVKFKEDTKV DEILKGLENL VSQIDTVKSF EWGEDKESHD MLRQGFTHAF SMTFENKDGY VAFTSHPLHV EFSAAFTAVI DKIVLLDFPV AAVKSSVVAT P

Total weight

24697.9 Da

Max. entity weight

12348.95 Da

Source organism

Arabidopsis thaliana

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.1 %, Completeness: 84.7 %, Completeness (bb): 97.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	84.7 % (1098 of 1297)	84.0 % (555 of 661)	83.0 % (434 of 523)	96.5 % (109 of 113)
Backbone	97.0 % (640 of 660)	96.9 % (217 of 224)	97.0 % (318 of 328)	97.2 % (105 of 108)
Sidechain	75.4 % (560 of 743)	77.3 % (338 of 437)	72.4 % (218 of 301)	80.0 % (4 of 5)
Aromatic	7.9 % (11 of 140)	7.1 % (5 of 70)	7.2 % (5 of 69)	100.0 % (1 of 1)
Methyl	97.1 % (134 of 138)	97.1 % (67 of 69)	97.1 % (67 of 69)

1. At5g22580

MATSGFKHLV VVKFKEDTKV DEILKGLENL VSQIDTVKSF EWGEDKESHD MLRQGFTHAF SMTFENKDGY VAFTSHPLHV EFSAAFTAVI DKIVLLDFPV AAVKSSVVAT P

Show sample condition

Sample

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	At5g22580	[U-95% 13C; U-90% 15N]	1.0 mM
2	NaPi		50 mM
3	NaN3		0.1 mM
4	H2O		93 %
5	D2O		7 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.2 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.2 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.02 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.22 ppm, Outliers: 4 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 1RJJ, Strand ID: A, B Detail

Release date

2003-12-18

Citation

Solution structure of a homodimeric hypothetical protein, At5g22580, a structural genomics target from Arabidopsis thaliana
Cornilescu, G., Cornilescu, C.C., Zhao, Q., Frederick, R.O., Peterson, F.C., Thao, S., Markley, J.L.
J. Biomol. NMR (2004), 29, 387-390, PubMed 15213437 , DOI 10.1023/B:JNMR.0000032525.70677.16 , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Swiss-Prot: Q9FK81 released on 2005-08-30
Title Y5258_ARATH Entity Uncharacterized protein At5g22580

Related entities 1. At5g22580, : 1 : 2 : 42 entities Detail

Experiments performed 15 experiments Detail

1 HN-HSQC

Instrument

#1 Model Varian INOVA (600 MHz)

#2 Model Varian INOVA (800 MHz)

#3 Model Bruker Avance (750 MHz)

#4 Model Bruker Avance (500 MHz)

Sample #1

pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	At5g22580	[U-95% 13C; U-90% 15N]	1.0 mM
2	NaPi		50 mM
3	NaN3		0.1 mM
4	H2O		93 %
5	D2O		7 %

Sample #2

pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
6	At5g22580	[U-95% 13C; U-90% 15N]		0.3 mM
7	C12E5 polyethylene glycol/hexanol, 0.96/1.0			5 %

2 IPAP-HSQC (align)

Instrument

#1 Model Varian INOVA (600 MHz)

#2 Model Varian INOVA (800 MHz)

#3 Model Bruker Avance (750 MHz)

#4 Model Bruker Avance (500 MHz)

Sample #1

pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	At5g22580	[U-95% 13C; U-90% 15N]	1.0 mM
2	NaPi		50 mM
3	NaN3		0.1 mM
4	H2O		93 %
5	D2O		7 %

Sample #2

pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
6	At5g22580	[U-95% 13C; U-90% 15N]		0.3 mM
7	C12E5 polyethylene glycol/hexanol, 0.96/1.0			5 %

3 HNCO

Instrument

#1 Model Varian INOVA (600 MHz)

#2 Model Varian INOVA (800 MHz)

#3 Model Bruker Avance (750 MHz)

#4 Model Bruker Avance (500 MHz)

Sample #1

pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	At5g22580	[U-95% 13C; U-90% 15N]	1.0 mM
2	NaPi		50 mM
3	NaN3		0.1 mM
4	H2O		93 %
5	D2O		7 %

Sample #2

pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
6	At5g22580	[U-95% 13C; U-90% 15N]		0.3 mM
7	C12E5 polyethylene glycol/hexanol, 0.96/1.0			5 %

4 HNCACB

Instrument

#1 Model Varian INOVA (600 MHz)

#2 Model Varian INOVA (800 MHz)

#3 Model Bruker Avance (750 MHz)

#4 Model Bruker Avance (500 MHz)

Sample #1

pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	At5g22580	[U-95% 13C; U-90% 15N]	1.0 mM
2	NaPi		50 mM
3	NaN3		0.1 mM
4	H2O		93 %
5	D2O		7 %

Sample #2

pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
6	At5g22580	[U-95% 13C; U-90% 15N]		0.3 mM
7	C12E5 polyethylene glycol/hexanol, 0.96/1.0			5 %

5 CBCA(CO)NH

Instrument

#1 Model Varian INOVA (600 MHz)

#2 Model Varian INOVA (800 MHz)

#3 Model Bruker Avance (750 MHz)

#4 Model Bruker Avance (500 MHz)

Sample #1

pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	At5g22580	[U-95% 13C; U-90% 15N]	1.0 mM
2	NaPi		50 mM
3	NaN3		0.1 mM
4	H2O		93 %
5	D2O		7 %

Sample #2

pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
6	At5g22580	[U-95% 13C; U-90% 15N]		0.3 mM
7	C12E5 polyethylene glycol/hexanol, 0.96/1.0			5 %

6 HBHA(CO)NH

Instrument

#1 Model Varian INOVA (600 MHz)

#2 Model Varian INOVA (800 MHz)

#3 Model Bruker Avance (750 MHz)

#4 Model Bruker Avance (500 MHz)

Sample #1

pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	At5g22580	[U-95% 13C; U-90% 15N]	1.0 mM
2	NaPi		50 mM
3	NaN3		0.1 mM
4	H2O		93 %
5	D2O		7 %

Sample #2

pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
6	At5g22580	[U-95% 13C; U-90% 15N]		0.3 mM
7	C12E5 polyethylene glycol/hexanol, 0.96/1.0			5 %

7 C(CO)NH

Instrument

#1 Model Varian INOVA (600 MHz)

#2 Model Varian INOVA (800 MHz)

#3 Model Bruker Avance (750 MHz)

#4 Model Bruker Avance (500 MHz)

Sample #1

pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	At5g22580	[U-95% 13C; U-90% 15N]	1.0 mM
2	NaPi		50 mM
3	NaN3		0.1 mM
4	H2O		93 %
5	D2O		7 %

Sample #2

pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
6	At5g22580	[U-95% 13C; U-90% 15N]		0.3 mM
7	C12E5 polyethylene glycol/hexanol, 0.96/1.0			5 %

8 H(CCO)NH

Instrument

#1 Model Varian INOVA (600 MHz)

#2 Model Varian INOVA (800 MHz)

#3 Model Bruker Avance (750 MHz)

#4 Model Bruker Avance (500 MHz)

Sample #1

pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	At5g22580	[U-95% 13C; U-90% 15N]	1.0 mM
2	NaPi		50 mM
3	NaN3		0.1 mM
4	H2O		93 %
5	D2O		7 %

Sample #2

pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
6	At5g22580	[U-95% 13C; U-90% 15N]		0.3 mM
7	C12E5 polyethylene glycol/hexanol, 0.96/1.0			5 %

9 HCCH-TOCSY

Instrument

#1 Model Varian INOVA (600 MHz)

#2 Model Varian INOVA (800 MHz)

#3 Model Bruker Avance (750 MHz)

#4 Model Bruker Avance (500 MHz)

Sample #1

pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	At5g22580	[U-95% 13C; U-90% 15N]	1.0 mM
2	NaPi		50 mM
3	NaN3		0.1 mM
4	H2O		93 %
5	D2O		7 %

Sample #2

pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
6	At5g22580	[U-95% 13C; U-90% 15N]		0.3 mM
7	C12E5 polyethylene glycol/hexanol, 0.96/1.0			5 %

10 3D-C13-NOE

Instrument

#1 Model Varian INOVA (600 MHz)

#2 Model Varian INOVA (800 MHz)

#3 Model Bruker Avance (750 MHz)

#4 Model Bruker Avance (500 MHz)

Sample #1

pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	At5g22580	[U-95% 13C; U-90% 15N]	1.0 mM
2	NaPi		50 mM
3	NaN3		0.1 mM
4	H2O		93 %
5	D2O		7 %

Sample #2

pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
6	At5g22580	[U-95% 13C; U-90% 15N]		0.3 mM
7	C12E5 polyethylene glycol/hexanol, 0.96/1.0			5 %

11 3D-N15-NOE

Instrument

#1 Model Varian INOVA (600 MHz)

#2 Model Varian INOVA (800 MHz)

#3 Model Bruker Avance (750 MHz)

#4 Model Bruker Avance (500 MHz)

Sample #1

pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	At5g22580	[U-95% 13C; U-90% 15N]	1.0 mM
2	NaPi		50 mM
3	NaN3		0.1 mM
4	H2O		93 %
5	D2O		7 %

Sample #2

pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
6	At5g22580	[U-95% 13C; U-90% 15N]		0.3 mM
7	C12E5 polyethylene glycol/hexanol, 0.96/1.0			5 %

12 diffusion (H2O)

Instrument

#1 Model Varian INOVA (600 MHz)

#2 Model Varian INOVA (800 MHz)

#3 Model Bruker Avance (750 MHz)

#4 Model Bruker Avance (500 MHz)

Sample #1

pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	At5g22580	[U-95% 13C; U-90% 15N]	1.0 mM
2	NaPi		50 mM
3	NaN3		0.1 mM
4	H2O		93 %
5	D2O		7 %

Sample #2

pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
6	At5g22580	[U-95% 13C; U-90% 15N]		0.3 mM
7	C12E5 polyethylene glycol/hexanol, 0.96/1.0			5 %

13 N15-T2

Instrument

#1 Model Varian INOVA (600 MHz)

#2 Model Varian INOVA (800 MHz)

#3 Model Bruker Avance (750 MHz)

#4 Model Bruker Avance (500 MHz)

Sample #1

pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	At5g22580	[U-95% 13C; U-90% 15N]	1.0 mM
2	NaPi		50 mM
3	NaN3		0.1 mM
4	H2O		93 %
5	D2O		7 %

Sample #2

pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
6	At5g22580	[U-95% 13C; U-90% 15N]		0.3 mM
7	C12E5 polyethylene glycol/hexanol, 0.96/1.0			5 %

14 diffusion (D2O)

Instrument

#1 Model Varian INOVA (600 MHz)

#2 Model Varian INOVA (800 MHz)

#3 Model Bruker Avance (750 MHz)

#4 Model Bruker Avance (500 MHz)

Sample #1

pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	At5g22580	[U-95% 13C; U-90% 15N]	1.0 mM
2	NaPi		50 mM
3	NaN3		0.1 mM
4	H2O		93 %
5	D2O		7 %

Sample #2

pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
6	At5g22580	[U-95% 13C; U-90% 15N]		0.3 mM
7	C12E5 polyethylene glycol/hexanol, 0.96/1.0			5 %

15 IPAP-HSQC (iso)

Instrument

#1 Model Varian INOVA (600 MHz)

#2 Model Varian INOVA (800 MHz)

#3 Model Bruker Avance (750 MHz)

#4 Model Bruker Avance (500 MHz)

Sample #1

pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	At5g22580	[U-95% 13C; U-90% 15N]	1.0 mM
2	NaPi		50 mM
3	NaN3		0.1 mM
4	H2O		93 %
5	D2O		7 %

Sample #2

pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
6	At5g22580	[U-95% 13C; U-90% 15N]		0.3 mM
7	C12E5 polyethylene glycol/hexanol, 0.96/1.0			5 %

nullKeywords At5g22580, CESG, Center for Eukaryotic Structural Genomics, homodimer, residual dipolar couplings

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Found 1 Document (1.938 seconds)

BMRB: 6011

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Entries sharing articles Swiss-Prot: 1 entries Detail

Related entities 1. At5g22580, : 1 : 2 : 42 entities Detail

Experiments performed 15 experiments Detail

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