BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	85.0 % (1199 of 1410)	81.5 % (604 of 741)	88.5 % (484 of 547)	91.0 % (111 of 122)
Backbone	92.4 % (621 of 672)	92.5 % (209 of 226)	92.3 % (312 of 338)	92.6 % (100 of 108)
Sidechain	80.4 % (682 of 848)	76.7 % (395 of 515)	86.5 % (276 of 319)	78.6 % (11 of 14)
Aromatic	60.0 % (54 of 90)	60.0 % (27 of 45)	58.1 % (25 of 43)	100.0 % (2 of 2)
Methyl	93.5 % (129 of 138)	94.2 % (65 of 69)	92.8 % (64 of 69)

1. Sgt1 CS

GSHMTHQSKI KYDWYQTESQ VVITLMIKNV QKNDVNVEFS EKELSALVKL PSGEDYNLKL ELLHPIIPEQ STFKVLSTKI EIKLKKPEAV RWEKLEGQGD VPTPKQFVAD VKNL

Show sample condition

Sample

Temperature 303 (±1) K, pH 4.5 (±0.2)

#	Name	Isotope labeling	Concentration
1	Sgt1 CS	[U-15N]	1.0 ~ 2.0 mM
2	H2O		93 %
3	D2O		7 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.02 ppm, Outliers: 4 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.02 ppm, Outliers: 4 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.14 ppm, Outliers: 3 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 1.04 ppm, Outliers: 3 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 1RL1, Strand ID: A Detail

Release date

2004-05-14

Citation

Human Sgt1 binds HSP90 through the CHORD-Sgt1 domain and not the tetratricopeptide repeat domain
Lee, Y., Jacob, J., Michowski, W., Nowotny, M., Kuznicki, J., Chazin, W.J.
J. Biol. Chem. (2004), 279, 16511-16517, PubMed 14761955 , DOI 10.1074/jbc.M400215200 , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Swiss-Prot: Q9Y2Z0 released on 2003-10-03
Title SUGT1_HUMAN Entity Suppressor of G2 allele of SKP1 homolog

Related entities 1. Sgt1 CS domain, : 1 : 2 : 45 entities Detail

Interaction partners 1. Sgt1 CS domain, : 56 interactors Detail

More details for 56 interactors identified by 21 citations

Experiments performed 4 experiments Detail

1 1-2 MM SGT1 CS DOMAIN U-15N, 20 MM ACETIC ACID, 5 MM DTT, 93% H2O, 7% D2O

Instrument

Model Bruker Avance (600 MHz)

Sample #1

Temperature 303 (±1) K, pH 4.5 (±0.2)

#	Name	Isotope labeling	Concentration
1	Sgt1 CS	[U-15N]	1.0 ~ 2.0 mM
2	H2O		93 %
3	D2O		7 %

Sample #2

Temperature 303 (±1) K, pH 4.5 (±0.2)

#	Name	Isotope labeling	Concentration
4	Sgt1 CS	[U-15N; U-13C]	1.0 ~ 2.0 mM
5	H2O		93 %
6	D2O		7 %

Sample #3

Temperature 303 (±1) K, pH 4.5 (±0.2)

#	Name	Isotope labeling	Type	Concentration
7	Sgt1 CS	[U-13C]		1.0 ~ 2.0 mM
8	D2O			100 %

Sample #4

Temperature 303 (±1) K, pH 4.5 (±0.2)

#	Name	Isotope labeling	Type	Concentration
9	Sgt1 CS			1.0 ~ 2.0 mM
10	D2O			100 %

2 2D 15N-1H HSQC

Instrument

Model Bruker Avance (600 MHz)

Sample #1

Temperature 303 (±1) K, pH 4.5 (±0.2)

#	Name	Isotope labeling	Concentration
1	Sgt1 CS	[U-15N]	1.0 ~ 2.0 mM
2	H2O		93 %
3	D2O		7 %

Sample #2

Temperature 303 (±1) K, pH 4.5 (±0.2)

#	Name	Isotope labeling	Concentration
4	Sgt1 CS	[U-15N; U-13C]	1.0 ~ 2.0 mM
5	H2O		93 %
6	D2O		7 %

Sample #3

Temperature 303 (±1) K, pH 4.5 (±0.2)

#	Name	Isotope labeling	Type	Concentration
7	Sgt1 CS	[U-13C]		1.0 ~ 2.0 mM
8	D2O			100 %

Sample #4

Temperature 303 (±1) K, pH 4.5 (±0.2)

#	Name	Isotope labeling	Type	Concentration
9	Sgt1 CS			1.0 ~ 2.0 mM
10	D2O			100 %

3 3D 15N-edited NOESY

Instrument

Model Bruker Avance (600 MHz)

Sample #1

Temperature 303 (±1) K, pH 4.5 (±0.2)

#	Name	Isotope labeling	Concentration
1	Sgt1 CS	[U-15N]	1.0 ~ 2.0 mM
2	H2O		93 %
3	D2O		7 %

Sample #2

Temperature 303 (±1) K, pH 4.5 (±0.2)

#	Name	Isotope labeling	Concentration
4	Sgt1 CS	[U-15N; U-13C]	1.0 ~ 2.0 mM
5	H2O		93 %
6	D2O		7 %

Sample #3

Temperature 303 (±1) K, pH 4.5 (±0.2)

#	Name	Isotope labeling	Type	Concentration
7	Sgt1 CS	[U-13C]		1.0 ~ 2.0 mM
8	D2O			100 %

Sample #4

Temperature 303 (±1) K, pH 4.5 (±0.2)

#	Name	Isotope labeling	Type	Concentration
9	Sgt1 CS			1.0 ~ 2.0 mM
10	D2O			100 %

4 1-2 MM SGT1 CS DOMAIN U-15N,U-13C, 20 MM ACETIC ACID, 5 MM DTT, 93% H2O, 7% D2O

Instrument

Model Bruker Avance (600 MHz)

Sample #1

Temperature 303 (±1) K, pH 4.5 (±0.2)

#	Name	Isotope labeling	Concentration
1	Sgt1 CS	[U-15N]	1.0 ~ 2.0 mM
2	H2O		93 %
3	D2O		7 %

Sample #2

Temperature 303 (±1) K, pH 4.5 (±0.2)

#	Name	Isotope labeling	Concentration
4	Sgt1 CS	[U-15N; U-13C]	1.0 ~ 2.0 mM
5	H2O		93 %
6	D2O		7 %

Sample #3

Temperature 303 (±1) K, pH 4.5 (±0.2)

#	Name	Isotope labeling	Type	Concentration
7	Sgt1 CS	[U-13C]		1.0 ~ 2.0 mM
8	D2O			100 %

Sample #4

Temperature 303 (±1) K, pH 4.5 (±0.2)

#	Name	Isotope labeling	Type	Concentration
9	Sgt1 CS			1.0 ~ 2.0 mM
10	D2O			100 %

nullKeywords CS domain, HSP90, p23, Sgt1, TPR

Search

Query history

Resources

Search for primary databases

Search for subsidiary databases

Display

Hits per page

Statistics

Query Changes will take effect after "OK" button is selected.

Maximum hits

Default conjunction operator

Omni-box

Search filter

Sequence search (BLAST)

E-value

Query sequence coverage

Miscellaneous

The number of previewed publications

Completeness of assigned chemical shift

Sequence chart about top aligned regions

Sequence chart about interacting regions

Hit context with highlight

Link destinations for BMRB, PDB and EMDB

Found 1 Document (3.39 seconds)

BMRB: 6012

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Entries sharing articles Swiss-Prot: 1 entries Detail

Related entities 1. Sgt1 CS domain, : 1 : 2 : 45 entities Detail

Interaction partners 1. Sgt1 CS domain, : 56 interactors Detail

Experiments performed 4 experiments Detail

Instrument

Sample #1

Sample #2

Sample #3

Sample #4

Instrument

Sample #1

Sample #2

Sample #3

Sample #4

Instrument

Sample #1

Sample #2

Sample #3

Sample #4

Instrument

Sample #1

Sample #2

Sample #3

Sample #4