BMRB: 6014

1H, 13C and 15N backbone resonance assignments of matrilysin (mmp7) complexed with a hydroxamic acid inhibitor

Authors

Ou, L., Zhang, Q., Ma, J., Wang, Z., Zhang, F., Wu, H.

Assembly

matrilysin

Entity

1. matrilysin (polymer, Thiol state: not present), 173 monomers, 19130.23 Da Detail

YSLFPNSPKW TSKVVTYRIV SYTRDLPHIT VDRLVSKALN MWGKEIPLHF RKVVWGTADI MIGFARGAHG DSYPFDGPGN TLAHAFAPGT GLGGDAHFDE DERWTDGSSL GINFLYAATH ELGHSLGMGH SSDPNAVMYP TYGNGDPQNF KLSQDDIKGI QKLYGKRSNS RKK

2. MDW (non-polymer), 408.426 Da
3. ZN (non-polymer), 65.409 Da

Total weight

19604.064 Da

Max. entity weight

19130.23 Da

Source organism

Escherichia coli

Exptl. method

NMR

Refine. method

distance geometry

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.4 %, Completeness: 50.1 %, Completeness (bb): 91.2 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	50.1 % (995 of 1986)	34.6 % (356 of 1028)	62.7 % (489 of 780)	84.3 % (150 of 178)
Backbone	91.2 % (928 of 1018)	87.6 % (312 of 356)	93.6 % (467 of 499)	91.4 % (149 of 163)
Sidechain	19.2 % (215 of 1121)	6.5 % (44 of 672)	39.2 % (170 of 434)	6.7 % (1 of 15)
Aromatic	5.4 % (12 of 224)	5.4 % (6 of 112)	4.6 % (5 of 108)	25.0 % (1 of 4)
Methyl	19.6 % (31 of 158)	12.7 % (10 of 79)	26.6 % (21 of 79)

1. matrilysin

YSLFPNSPKW TSKVVTYRIV SYTRDLPHIT VDRLVSKALN MWGKEIPLHF RKVVWGTADI MIGFARGAHG DSYPFDGPGN TLAHAFAPGT GLGGDAHFDE DERWTDGSSL GINFLYAATH ELGHSLGMGH SSDPNAVMYP TYGNGDPQNF KLSQDDIKGI QKLYGKRSNS RKK

Show sample condition

Sample

Temperature 298 (±1) K, pH 6.9 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	matrilysin			0.6 ~ 0.7 mM
2	NaCl			100 mM
3	Tris-HCl			10 mM
4	CaCl2			10 mM
5	ZnCl2			0.1 mM
6	NaN3			2 mM

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LACS Plot; CA

Referencing offset: -0.78 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	H2O	protons	4.76 ppm	null	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; CB

Referencing offset: -0.78 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	H2O	protons	4.76 ppm	null	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; HA

Referencing offset: -0.08 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	H2O	protons	4.76 ppm	null	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; CO

Referencing offset: -0.49 ppm, Outliers: 3 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	H2O	protons	4.76 ppm	null	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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Protein Blocks Logo

Calculated from 25 models in PDB: 2DDY, Strand ID: A Detail

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Release date

2005-11-20

Citation

1H, 13C and 15N backbone resonance assignments of matrilysin (MMP7) complexed with a sulfonamide hydroxamate-type inhibitor
Ou, L., Zhang, Q., Ma, J., Wu, W., Wang, Z., Zhang, F., Ma, D., Wu, H.
J. Biomol. NMR (2004), 29, 537-538, PubMed 15243187 , DOI 10.1023/B:JNMR.0000034357.96939.3c , Abstract

Related entities 1. matrilysin, : 1 : 3 : 5 : 9 : 265 entities Detail

Interaction partners 1. matrilysin, : 3 interactors Detail

Experiments performed 8 experiments Detail