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1H, 13C and 15N backbone resonance assignments of matrilysin (mmp7) complexed with a hydroxamic acid inhibitor
Authors
Ou, L., Zhang, Q., Ma, J., Wang, Z., Zhang, F., Wu, H.
Assembly
matrilysin
Entity
1. matrilysin (polymer, Thiol state: not present), 173 monomers, 19130.23 Da Detail

YSLFPNSPKW TSKVVTYRIV SYTRDLPHIT VDRLVSKALN MWGKEIPLHF RKVVWGTADI MIGFARGAHG DSYPFDGPGN TLAHAFAPGT GLGGDAHFDE DERWTDGSSL GINFLYAATH ELGHSLGMGH SSDPNAVMYP TYGNGDPQNF KLSQDDIKGI QKLYGKRSNS RKK


2. MDW (non-polymer), 408.426 Da
3. ZN (non-polymer), 65.409 Da
Total weight
19604.064 Da
Max. entity weight
19130.23 Da
Source organism
Escherichia coli
Exptl. method
NMR
Refine. method
distance geometry
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 99.4 %, Completeness: 50.1 %, Completeness (bb): 91.2 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All50.1 % (995 of 1986)34.6 % (356 of 1028)62.7 % (489 of 780)84.3 % (150 of 178)
Backbone91.2 % (928 of 1018)87.6 % (312 of 356)93.6 % (467 of 499)91.4 % (149 of 163)
Sidechain19.2 % (215 of 1121) 6.5 % (44 of 672)39.2 % (170 of 434) 6.7 % (1 of 15)
Aromatic 5.4 % (12 of 224) 5.4 % (6 of 112) 4.6 % (5 of 108)25.0 % (1 of 4)
Methyl19.6 % (31 of 158)12.7 % (10 of 79)26.6 % (21 of 79)

1. matrilysin

YSLFPNSPKW TSKVVTYRIV SYTRDLPHIT VDRLVSKALN MWGKEIPLHF RKVVWGTADI MIGFARGAHG DSYPFDGPGN TLAHAFAPGT GLGGDAHFDE DERWTDGSSL GINFLYAATH ELGHSLGMGH SSDPNAVMYP TYGNGDPQNF KLSQDDIKGI QKLYGKRSNS RKK

Sample

Temperature 298 (±1) K, pH 6.9 (±0.2)


#NameIsotope labelingTypeConcentration
1matrilysin0.6 ~ 0.7 mM
2NaCl100 mM
3Tris-HCl10 mM
4CaCl210 mM
5ZnCl20.1 mM
6NaN32 mM

LACS Plot; CA
Referencing offset: -0.78 ppm, Outliers: 1 Detail
LACS Plot; CB
Referencing offset: -0.78 ppm, Outliers: 1 Detail
LACS Plot; HA
Referencing offset: -0.08 ppm, Outliers: 1 Detail
LACS Plot; CO
Referencing offset: -0.49 ppm, Outliers: 3 Detail
Protein Blocks Logo
Calculated from 25 models in PDB: 2DDY, Strand ID: A Detail


Release date
2005-11-20
Citation
1H, 13C and 15N backbone resonance assignments of matrilysin (MMP7) complexed with a sulfonamide hydroxamate-type inhibitor
Ou, L., Zhang, Q., Ma, J., Wu, W., Wang, Z., Zhang, F., Ma, D., Wu, H.
J. Biomol. NMR (2004), 29, 537-538, PubMed 15243187 , DOI 10.1023/B:JNMR.0000034357.96939.3c ,
Related entities 1. matrilysin, : 1 : 3 : 5 : 9 : 265 entities Detail
Interaction partners 1. matrilysin, : 3 interactors Detail
Experiments performed 8 experiments Detail
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