BMRB: 6015

Sequence specific assignment of C1 domain of human cardiac myosin binding protein C

Authors

Ababou, A., Pfuhl, M.

Assembly

cC1 domain

Entity

1. cC1 domain (polymer, Thiol state: all free), 120 monomers, 13465.10 Da Detail

MHHHHHHSSM DDPIGLFVMR PQDGEVTVGG SITFSARVAG ASLLKPPVVK WFKGKWVDLS SKVGQHLQLH DSYDRASKVY LFELHITDAQ PAFTGGYRCE VSTKDKFDCS NFNLTVHEAM

Formula weight

13465.1 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

simulated annealing using ARIA program

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 93.3 %, Completeness: 79.7 %, Completeness (bb): 90.8 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	79.7 % (1104 of 1386)	78.0 % (556 of 713)	79.1 % (435 of 550)	91.9 % (113 of 123)
Backbone	90.8 % (645 of 710)	91.8 % (224 of 244)	90.0 % (316 of 351)	91.3 % (105 of 115)
Sidechain	71.4 % (562 of 787)	70.8 % (332 of 469)	71.6 % (222 of 310)	100.0 % (8 of 8)
Aromatic	30.4 % (48 of 158)	34.2 % (27 of 79)	24.7 % (19 of 77)	100.0 % (2 of 2)
Methyl	92.4 % (109 of 118)	93.2 % (55 of 59)	91.5 % (54 of 59)

1. cC1 domain

MHHHHHHSSM DDPIGLFVMR PQDGEVTVGG SITFSARVAG ASLLKPPVVK WFKGKWVDLS SKVGQHLQLH DSYDRASKVY LFELHITDAQ PAFTGGYRCE VSTKDKFDCS NFNLTVHEAM

Show sample condition

Sample

Temperature 298 (±0.2) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	cC1 domain			0.90 mM

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LACS Plot; CA

Referencing offset: -0.4 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; CB

Referencing offset: -0.4 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; HA

Referencing offset: -0.04 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; CO

Referencing offset: -1.47 ppm, Outliers: 5 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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Protein Blocks Logo

Calculated from 29 models in PDB: 2AVG, Strand ID: A Detail

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Release date

2004-07-05

Citation

Sequence specific assignment of domain C1 of the N-terminal myosin-binding site of human cardiac myosin binding protein C (MyBP-C)
Ababou, A., Zhou, L., Gautel, M., Pfuhl, M.
J. Biomol. NMR (2004), 29, 431-432, PubMed 15213454 , DOI 10.1023/B:JNMR.0000032510.03606.63 , Abstract

Related entities 1. cC1 domain, : 1 : 14 : 146 entities Detail

Interaction partners 1. cC1 domain, : 5 interactors Detail

Experiments performed 14 experiments Detail