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BMRB: 6028

1RQ6

Northeast Structural Genomics Consortium Target TT802 Ontario Center for Structural Proteomics Target Mth0803 assignment of ribosomal protein S17E

Authors

Wu, B., Yee, A., Ramelot, T., Cort, J.R., Kennedy, M., Arrowsmith, C.H.

Assembly

ribosomal protein S17E

Entity

1. ribosomal protein S17E (polymer, Thiol state: not present), 62 monomers, 7199.228 Da Detail

MGNIRTSFVK RIAKEMIETH PGKFTDDFDT NKKLVEEFST VSTKHLRNKI AGYITRIISQ QK

Formula weight

7199.228 Da

Source organism

Methanothermobacter thermautotrophicus

Exptl. method

Refine. method

torsion angle dyanmics

Data set

assigned_chemical_shifts, coupling_constants

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 89.6 %, Completeness (bb): 95.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	89.6 % (682 of 761)	90.2 % (361 of 400)	87.8 % (259 of 295)	93.9 % (62 of 66)
Backbone	95.4 % (353 of 370)	95.2 % (120 of 126)	95.6 % (175 of 183)	95.1 % (58 of 61)
Sidechain	85.8 % (386 of 450)	88.0 % (241 of 274)	82.5 % (141 of 171)	80.0 % (4 of 5)
Aromatic	41.1 % (23 of 56)	53.6 % (15 of 28)	28.6 % (8 of 28)
Methyl	98.5 % (65 of 66)	100.0 % (33 of 33)	97.0 % (32 of 33)

1. ribosomal protein S17E

MGNIRTSFVK RIAKEMIETH PGKFTDDFDT NKKLVEEFST VSTKHLRNKI AGYITRIISQ QK

Show sample condition

Sample

Temperature 298 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	ribosomal protein S17E	[U-13C; U-15N]		1 mM
2	NaCL			450 mM

LACS Plot; CA

Referencing offset: -0.74 ppm, Outliers: 3 Detail

LACS Plot; CB

Referencing offset: -0.74 ppm, Outliers: 3 Detail

LACS Plot; HA

Referencing offset: -0.09 ppm, Outliers: 1 Detail

LACS Plot; CO

Referencing offset: -0.66 ppm, Outliers: 3 Detail

Protein Blocks Logo

Calculated from 15 models in PDB: 1RQ6, Strand ID: A Detail

Coupling constant

49 J values in 1 lists
Temperature 298 (±1) K, pH 6.5 (±0.2) Detail

Release date

2004-05-14

Citation

The solution structure of ribosomal protein S17E from Methanobacterium thermoautotrophicum: a structural homolog of the FF domain
Wu, B., Yee, A., Ramelot, T., Cort, J.R., Kennedy, M., Arrowsmith, C.H.
Protein Sci. (2008), 17, 583-588, PubMed 18218711 , DOI 10.1110/ps.073272208 , Abstract

Related entities 1. ribosomal protein S17E, : 1 : 1 : 224 entities Detail

Experiments performed 15 experiments Detail

1 15N HSQC

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Temperature 298 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	ribosomal protein S17E	[U-13C; U-15N]		1 mM
2	NaCL			450 mM

2 HNCO

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Temperature 298 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	ribosomal protein S17E	[U-13C; U-15N]		1 mM
2	NaCL			450 mM

3 CBCACONH

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Temperature 298 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	ribosomal protein S17E	[U-13C; U-15N]		1 mM
2	NaCL			450 mM

4 HNCACB

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Temperature 298 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	ribosomal protein S17E	[U-13C; U-15N]		1 mM
2	NaCL			450 mM

5 HNCA

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Temperature 298 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	ribosomal protein S17E	[U-13C; U-15N]		1 mM
2	NaCL			450 mM

6 HNCOCA

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Temperature 298 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	ribosomal protein S17E	[U-13C; U-15N]		1 mM
2	NaCL			450 mM

7 CCTOCSY NH

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Temperature 298 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	ribosomal protein S17E	[U-13C; U-15N]		1 mM
2	NaCL			450 mM

8 HCCTOCSY NH

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Temperature 298 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	ribosomal protein S17E	[U-13C; U-15N]		1 mM
2	NaCL			450 mM

9 HNHA

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Temperature 298 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	ribosomal protein S17E	[U-13C; U-15N]		1 mM
2	NaCL			450 mM

10 13C HSQC

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Temperature 298 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	ribosomal protein S17E	[U-13C; U-15N]		1 mM
2	NaCL			450 mM

11 HCCHCOSY

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Temperature 298 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	ribosomal protein S17E	[U-13C; U-15N]		1 mM
2	NaCL			450 mM

12 HCCHTOCSY

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Temperature 298 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	ribosomal protein S17E	[U-13C; U-15N]		1 mM
2	NaCL			450 mM

13 15N NOESY

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Temperature 298 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	ribosomal protein S17E	[U-13C; U-15N]		1 mM
2	NaCL			450 mM

14 13C NOESY

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Temperature 298 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	ribosomal protein S17E	[U-13C; U-15N]		1 mM
2	NaCL			450 mM

15 13,13C 4D CCNOESY

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Temperature 298 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	ribosomal protein S17E	[U-13C; U-15N]		1 mM
2	NaCL			450 mM

nullKeywords ribosomal protein S17E