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BMRB: 6029

1U7Q

1H, 13C and 15N resonance assignment of the nucleotide binding domain of KdpB from Escherichia coli

Authors

Haupt, M., Coles, M., Truffault, V., Bramkamp, M., Kessler, H., Altendorf, K.

Assembly

Type IA potassium translocating ATPase B chain

Entity

1. Type IA potassium translocating ATPase B chain (polymer, Thiol state: not present), 156 monomers, 17134.01 Da Detail

MGHHHHHHHH HHSSGHGGRH NRQASEFIPA QGVDEKTLAD AAQLASLADE TPEGRSIVIL AKQRFNLRER DVQSLHATFV PFTAQSRMSG INIDNRMIRK GSVDAIRRHV EANGGHFPTD VDQKVDQVAR QGATPLVVVE GSRVLGVIAL KDIVKG

Formula weight

17134.01 Da

Source organism

Escherichia coli

Exptl. method

NMR

Refine. method

SIMULATED ANNEALING

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 89.1 %, Completeness: 85.0 %, Completeness (bb): 87.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	85.0 % (1483 of 1744)	86.2 % (779 of 904)	83.6 % (564 of 675)	84.8 % (140 of 165)
Backbone	87.0 % (806 of 926)	86.6 % (278 of 321)	87.2 % (396 of 454)	87.4 % (132 of 151)
Sidechain	83.7 % (804 of 960)	85.9 % (501 of 583)	81.3 % (295 of 363)	57.1 % (8 of 14)
Aromatic	32.7 % (36 of 110)	52.7 % (29 of 55)	12.7 % (7 of 55)
Methyl	100.0 % (174 of 174)	100.0 % (87 of 87)	100.0 % (87 of 87)

1. potassium transporting ATPase

MGHHHHHHHH HHSSGHGGRH NRQASEFIPA QGVDEKTLAD AAQLASLADE TPEGRSIVIL AKQRFNLRER DVQSLHATFV PFTAQSRMSG INIDNRMIRK GSVDAIRRHV EANGGHFPTD VDQKVDQVAR QGATPLVVVE GSRVLGVIAL KDIVKG

Show sample condition

Sample

Temperature 300 (±0.1) K, pH 6.0 (±0.02)

#	Name	Isotope labeling	Type	Concentration
1	Type IA potassium translocating ATPase B chain	[U-13C; U-15N]		1.2 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.03 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.03 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.02 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.15 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 1 models in PDB: 1SVJ, Strand ID: A Detail

Release date

2004-07-05

Citation

1H, 13C and 15N resonance assignment of the nucleotide binding domain of KdpB from Escherichia coli
Haupt, M., Coles, M., Truffault, V., Bramkamp, M., Altendorf, K., Kessler, H.
J. Biomol. NMR (2004), 29, 437-438, PubMed 15213457 , DOI 10.1023/B:JNMR.0000032512.08757.8b , Abstract

Related entities 1. Type IA potassium translocating ATPase B chain, : 1 : 3 : 11 : 86 entities Detail

Interaction partners 1. Type IA potassium translocating ATPase B chain, : 8 interactors Detail

More details for 8 interactors identified by 2 citations

Experiments performed 10 experiments Detail

1 CBCA(CO)NH