BMRBj - BMREntryMaster

Found 1 Document (1.687 seconds)

BMRB: 6032

1WM4

Complete 1H, 13C and 15N resonance assignments of coactosin, a cytoskeletal regulatory protein

Authors

Hellman, M.H.

Assembly

Coactosin (Mus musculus)

Entity

1. Coactosin (Mus musculus) (polymer, Thiol state: all free), 142 monomers, 15943.71 Da Detail

MATKIDKEAC RAAYNLVRDD GSAVIWVTFR YDGATIVPGD QGADYQHFIQ QCTDDVRLFA FVRFTTGDAM SKRSKFALIT WIGEDVSGLQ RAKTGTDKTL VKEVVQNFAK EFVISDRKEL EEDFIRSELK KAGGANYDAQ SE

Formula weight

15943.71 Da

Source organism

Mus musculus

Exptl. method

NMR

Refine. method

torsion angle dynamics, simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.3 %, Completeness: 96.1 %, Completeness (bb): 96.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	96.1 % (1574 of 1638)	96.3 % (815 of 846)	95.9 % (613 of 639)	95.4 % (146 of 153)
Backbone	96.4 % (819 of 850)	96.2 % (282 of 293)	96.9 % (403 of 416)	95.0 % (134 of 141)
Sidechain	96.0 % (883 of 920)	96.4 % (533 of 553)	95.2 % (338 of 355)	100.0 % (12 of 12)
Aromatic	100.0 % (150 of 150)	100.0 % (75 of 75)	100.0 % (73 of 73)	100.0 % (2 of 2)
Methyl	97.4 % (150 of 154)	97.4 % (75 of 77)	97.4 % (75 of 77)

1. Coactosin

MATKIDKEAC RAAYNLVRDD GSAVIWVTFR YDGATIVPGD QGADYQHFIQ QCTDDVRLFA FVRFTTGDAM SKRSKFALIT WIGEDVSGLQ RAKTGTDKTL VKEVVQNFAK EFVISDRKEL EEDFIRSELK KAGGANYDAQ SE

Show sample condition

Sample

Temperature 298 (±1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Coactosin	[U-15N; U-13C]		1.0 mM
2	NaCl			50 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
³¹P	DSS	methyl protons	null	indirect	0.4048086
¹³C	DSS	methyl protons	null	indirect	0.2514495
²H	DSS	methyl protons	null	indirect	0.1535061

Referencing offset: 0.52 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
³¹P	DSS	methyl protons	null	indirect	0.4048086
¹³C	DSS	methyl protons	null	indirect	0.2514495
²H	DSS	methyl protons	null	indirect	0.1535061

Referencing offset: 0.52 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
³¹P	DSS	methyl protons	null	indirect	0.4048086
¹³C	DSS	methyl protons	null	indirect	0.2514495
²H	DSS	methyl protons	null	indirect	0.1535061

Referencing offset: 0.08 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
³¹P	DSS	methyl protons	null	indirect	0.4048086
¹³C	DSS	methyl protons	null	indirect	0.2514495
²H	DSS	methyl protons	null	indirect	0.1535061

Referencing offset: 0.18 ppm, Outliers: 5 Detail

Protein Blocks Logo

Calculated from 15 models in PDB: 1WM4, Strand ID: A Detail

Release date

2004-08-02

Citation

(1)H, (13)C and (15)N resonance assignments of coactosin, a cytoskeletal regulatory protein
Hellman, M.H., Paavilainen, V., Annila, A., Lappalainen, P., Perttu, P.
J. Biomol. NMR (2004), 30, 365-366, PubMed 15756466 , DOI 10.1007/s10858-005-1068-x , Abstract

Related entities 1. Coactosin (Mus musculus), : 1 : 3 : 6 : 27 entities Detail

Interaction partners 1. Coactosin (Mus musculus), : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Experiments performed 14 experiments Detail

1 1HN-15N-HSQC

Instrument

#1 Model Varian Inova (600 MHz)

#2 Model Varian Inova (800 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Coactosin	[U-15N; U-13C]		1.0 mM
2	NaCl			50 mM

Sample #2

Temperature 298 (±1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Coactosin	[U-15N]		1.2 mM
4	NaCl			50 mM

2 HNCA

Instrument

#1 Model Varian Inova (600 MHz)

#2 Model Varian Inova (800 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Coactosin	[U-15N; U-13C]		1.0 mM
2	NaCl			50 mM

Sample #2

Temperature 298 (±1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Coactosin	[U-15N]		1.2 mM
4	NaCl			50 mM

3 HN(CO)CA

Instrument

#1 Model Varian Inova (600 MHz)

#2 Model Varian Inova (800 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Coactosin	[U-15N; U-13C]		1.0 mM
2	NaCl			50 mM

Sample #2

Temperature 298 (±1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Coactosin	[U-15N]		1.2 mM
4	NaCl			50 mM

4 iHNCA

Instrument

#1 Model Varian Inova (600 MHz)

#2 Model Varian Inova (800 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Coactosin	[U-15N; U-13C]		1.0 mM
2	NaCl			50 mM

Sample #2

Temperature 298 (±1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Coactosin	[U-15N]		1.2 mM
4	NaCl			50 mM

5 HNCACB

Instrument

#1 Model Varian Inova (600 MHz)

#2 Model Varian Inova (800 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Coactosin	[U-15N; U-13C]		1.0 mM
2	NaCl			50 mM

Sample #2

Temperature 298 (±1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Coactosin	[U-15N]		1.2 mM
4	NaCl			50 mM

6 CBCA(CO)NH

Instrument

#1 Model Varian Inova (600 MHz)

#2 Model Varian Inova (800 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Coactosin	[U-15N; U-13C]		1.0 mM
2	NaCl			50 mM

Sample #2

Temperature 298 (±1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Coactosin	[U-15N]		1.2 mM
4	NaCl			50 mM

7 HNCO

Instrument

#1 Model Varian Inova (600 MHz)

#2 Model Varian Inova (800 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Coactosin	[U-15N; U-13C]		1.0 mM
2	NaCl			50 mM

Sample #2

Temperature 298 (±1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Coactosin	[U-15N]		1.2 mM
4	NaCl			50 mM

8 HN(CA)CO

Instrument

#1 Model Varian Inova (600 MHz)

#2 Model Varian Inova (800 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Coactosin	[U-15N; U-13C]		1.0 mM
2	NaCl			50 mM

Sample #2

Temperature 298 (±1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Coactosin	[U-15N]		1.2 mM
4	NaCl			50 mM

9 HC(C)H-COSY

Instrument

#1 Model Varian Inova (600 MHz)

#2 Model Varian Inova (800 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Coactosin	[U-15N; U-13C]		1.0 mM
2	NaCl			50 mM

Sample #2

Temperature 298 (±1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Coactosin	[U-15N]		1.2 mM
4	NaCl			50 mM

10 H(C)CH-TOCSY

Instrument

#1 Model Varian Inova (600 MHz)

#2 Model Varian Inova (800 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Coactosin	[U-15N; U-13C]		1.0 mM
2	NaCl			50 mM

Sample #2

Temperature 298 (±1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Coactosin	[U-15N]		1.2 mM
4	NaCl			50 mM

11 (H)C(CO)NH

Instrument

#1 Model Varian Inova (600 MHz)

#2 Model Varian Inova (800 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Coactosin	[U-15N; U-13C]		1.0 mM
2	NaCl			50 mM

Sample #2

Temperature 298 (±1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Coactosin	[U-15N]		1.2 mM
4	NaCl			50 mM

12 H(C)(CO)NH

Instrument

#1 Model Varian Inova (600 MHz)

#2 Model Varian Inova (800 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Coactosin	[U-15N; U-13C]		1.0 mM
2	NaCl			50 mM

Sample #2

Temperature 298 (±1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Coactosin	[U-15N]		1.2 mM
4	NaCl			50 mM

13 NOESY-15N-HSQC

Instrument

#1 Model Varian Inova (600 MHz)

#2 Model Varian Inova (800 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Coactosin	[U-15N; U-13C]		1.0 mM
2	NaCl			50 mM

Sample #2

Temperature 298 (±1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Coactosin	[U-15N]		1.2 mM
4	NaCl			50 mM

14 NOESY-13C-HSQC

Instrument

#1 Model Varian Inova (600 MHz)

#2 Model Varian Inova (800 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Coactosin	[U-15N; U-13C]		1.0 mM
2	NaCl			50 mM

Sample #2

Temperature 298 (±1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Coactosin	[U-15N]		1.2 mM
4	NaCl			50 mM

nullKeywords actin, ADF-H domain, cytoskeleton, NMR assignments

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Found 1 Document (1.687 seconds)

BMRB: 6032

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. Coactosin (Mus musculus), : 1 : 3 : 6 : 27 entities Detail

Interaction partners 1. Coactosin (Mus musculus), : 1 interactors Detail

Experiments performed 14 experiments Detail

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