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Found 1 Document (6.777 seconds)

BMRB: 6034

1QTS

1H, 15N, 13C resonance assignments and 15N-1H residual dipolar couplings in 27 kDa alpha-adaptin ear-domain

Authors

Denisov, A.YU., Gehring, K.

Assembly

ear-domain of alpha-adaptin C

Entity

1. ear-domain of alpha-adaptin C (polymer, Thiol state: all free), 238 monomers, 26978.49 Da Detail

SEDNFARFVC KNNGVLFENQ LLQIGLKSEF RQNLGRMFIF YGNKTSTQFL NFTPTLICAD DLQTNLNLQT KPVDPTVDGG AQVQQVVNIE CISDFTEAPV LNIQFRYGGT FQNVSVKLPI TLNKFFQPTE MASQDFFQRW KQLSNPQQEV QNIFKAKHPM DTEITKAKII GFGSALLEEV DPNPANFVGA GIIHTKTTQI GCLLRLEPNL QAQMYRLTLR TSKDTVSQRL CELLSEQF

Formula weight

26978.49 Da

Source organism

Mus musculus

Exptl. method

NMR

Data set

assigned_chemical_shifts, RDCs

Chem. Shift Complete

Sequence coverage: 97.5 %, Completeness: 43.9 %, Completeness (bb): 78.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	43.9 % (1259 of 2871)	24.1 % (361 of 1498)	61.9 % (683 of 1104)	79.9 % (215 of 269)
Backbone	78.7 % (1107 of 1406)	50.4 % (241 of 478)	93.9 % (658 of 701)	91.6 % (208 of 227)
Sidechain	22.0 % (372 of 1690)	11.8 % (120 of 1020)	38.9 % (244 of 628)	19.0 % (8 of 42)
Aromatic	4.3 % (10 of 234)	7.7 % (9 of 117)	0.0 % (0 of 116)	100.0 % (1 of 1)
Methyl	16.9 % (46 of 272)	16.2 % (22 of 136)	17.6 % (24 of 136)

1. ear-domain of alpha-adaptin C

Show sample condition

Sample #1

Temperature 303 (±0.5) K, pH 7.3 (±0.1)

#	Name	Isotope labeling	Concentration
1	ear-domain of alpha-adaptin C	[U-99% 13C; U-99% 15N]	1.0 mM
2	sodium phosphate buffer		25 mM
3	NaCl		75 mM

Sample #2

Temperature 303 (±0.5) K, pH 7.3 (±0.1)

#	Name	Isotope labeling	Concentration
4	ear-domain of alpha-adaptin C	[U-99% 15N]	1.0 mM
5	sodium phosphate buffer		25 mM
6	NaCl		75 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.01 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.01 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.7 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 1 models in PDB: 1QTS, Strand ID: A Detail

RDC

205 RDC values in 1 lists
Field strength (¹H) 600 MHz, Temperature 303 (±0.5) K, pH 7.3 (±0.1) Detail

Release date

2004-06-24

Citation

1H, 15N and 13C resonance assignments and 15N-1H residual dipolar couplings for the alpha-adaptin ear-domain
Denisov, A.YU., Ritter, B., McPherson, P.S., Gehring, K.
J. Biomol. NMR (2004), 29, 441-442, PubMed 15213459 , DOI 10.1023/B:JNMR.0000032518.06803.e7 , Abstract

Related entities 1. ear-domain of alpha-adaptin C, : 1 : 8 : 5 : 3 : 30 entities Detail

Interaction partners 1. ear-domain of alpha-adaptin C, : 32 interactors Detail

More details for 32 interactors identified by 10 citations

Experiments performed 7 experiments Detail

1 3D HNCACB

Instrument

Model Bruker DRX (600 MHz) Details Cryoprobe was employed

Sample #1

Temperature 303 (±0.5) K, pH 7.3 (±0.1)

#	Name	Isotope labeling	Concentration
1	ear-domain of alpha-adaptin C	[U-99% 13C; U-99% 15N]	1.0 mM
2	sodium phosphate buffer		25 mM
3	NaCl		75 mM

Sample #2

Temperature 303 (±0.5) K, pH 7.3 (±0.1)

#	Name	Isotope labeling	Concentration
4	ear-domain of alpha-adaptin C	[U-99% 15N]	1.0 mM
5	sodium phosphate buffer		25 mM
6	NaCl		75 mM

Sample #3

Temperature 303 (±0.5) K, pH 7.3 (±0.1)

#	Name	Isotope labeling	Concentration
7	ear-domain of alpha-adaptin C	[U-99% 15N]	0.3 mM
8	sodium phosphate buffer		25 mM
9	NaCl		75 mM
10	Pf1 phage		2.5 mg/mL

2 3D CBCA(CO)HN

Instrument

Model Bruker DRX (600 MHz) Details Cryoprobe was employed

Sample #1

Temperature 303 (±0.5) K, pH 7.3 (±0.1)

#	Name	Isotope labeling	Concentration
1	ear-domain of alpha-adaptin C	[U-99% 13C; U-99% 15N]	1.0 mM
2	sodium phosphate buffer		25 mM
3	NaCl		75 mM

Sample #2

Temperature 303 (±0.5) K, pH 7.3 (±0.1)

#	Name	Isotope labeling	Concentration
4	ear-domain of alpha-adaptin C	[U-99% 15N]	1.0 mM
5	sodium phosphate buffer		25 mM
6	NaCl		75 mM

Sample #3

Temperature 303 (±0.5) K, pH 7.3 (±0.1)

#	Name	Isotope labeling	Concentration
7	ear-domain of alpha-adaptin C	[U-99% 15N]	0.3 mM
8	sodium phosphate buffer		25 mM
9	NaCl		75 mM
10	Pf1 phage		2.5 mg/mL

3 3D TROSY-HNCA

Instrument

Model Bruker DRX (600 MHz) Details Cryoprobe was employed

Sample #1

Temperature 303 (±0.5) K, pH 7.3 (±0.1)

#	Name	Isotope labeling	Concentration
1	ear-domain of alpha-adaptin C	[U-99% 13C; U-99% 15N]	1.0 mM
2	sodium phosphate buffer		25 mM
3	NaCl		75 mM

Sample #2

Temperature 303 (±0.5) K, pH 7.3 (±0.1)

#	Name	Isotope labeling	Concentration
4	ear-domain of alpha-adaptin C	[U-99% 15N]	1.0 mM
5	sodium phosphate buffer		25 mM
6	NaCl		75 mM

Sample #3

Temperature 303 (±0.5) K, pH 7.3 (±0.1)

#	Name	Isotope labeling	Concentration
7	ear-domain of alpha-adaptin C	[U-99% 15N]	0.3 mM
8	sodium phosphate buffer		25 mM
9	NaCl		75 mM
10	Pf1 phage		2.5 mg/mL

4 3D TROSY-HN(CO)CA

Instrument

Model Bruker DRX (600 MHz) Details Cryoprobe was employed

Sample #1

Temperature 303 (±0.5) K, pH 7.3 (±0.1)

#	Name	Isotope labeling	Concentration
1	ear-domain of alpha-adaptin C	[U-99% 13C; U-99% 15N]	1.0 mM
2	sodium phosphate buffer		25 mM
3	NaCl		75 mM

Sample #2

Temperature 303 (±0.5) K, pH 7.3 (±0.1)

#	Name	Isotope labeling	Concentration
4	ear-domain of alpha-adaptin C	[U-99% 15N]	1.0 mM
5	sodium phosphate buffer		25 mM
6	NaCl		75 mM

Sample #3

Temperature 303 (±0.5) K, pH 7.3 (±0.1)

#	Name	Isotope labeling	Concentration
7	ear-domain of alpha-adaptin C	[U-99% 15N]	0.3 mM
8	sodium phosphate buffer		25 mM
9	NaCl		75 mM
10	Pf1 phage		2.5 mg/mL

5 3D HNCO

Instrument

Model Bruker DRX (600 MHz) Details Cryoprobe was employed

Sample #1

Temperature 303 (±0.5) K, pH 7.3 (±0.1)

#	Name	Isotope labeling	Concentration
1	ear-domain of alpha-adaptin C	[U-99% 13C; U-99% 15N]	1.0 mM
2	sodium phosphate buffer		25 mM
3	NaCl		75 mM

Sample #2

Temperature 303 (±0.5) K, pH 7.3 (±0.1)

#	Name	Isotope labeling	Concentration
4	ear-domain of alpha-adaptin C	[U-99% 15N]	1.0 mM
5	sodium phosphate buffer		25 mM
6	NaCl		75 mM

Sample #3

Temperature 303 (±0.5) K, pH 7.3 (±0.1)

#	Name	Isotope labeling	Concentration
7	ear-domain of alpha-adaptin C	[U-99% 15N]	0.3 mM
8	sodium phosphate buffer		25 mM
9	NaCl		75 mM
10	Pf1 phage		2.5 mg/mL

6 3D 15N-HSQC-NOESY

Instrument

Model Bruker DRX (600 MHz) Details Cryoprobe was employed

Sample #1

Temperature 303 (±0.5) K, pH 7.3 (±0.1)

#	Name	Isotope labeling	Concentration
1	ear-domain of alpha-adaptin C	[U-99% 13C; U-99% 15N]	1.0 mM
2	sodium phosphate buffer		25 mM
3	NaCl		75 mM

Sample #2

Temperature 303 (±0.5) K, pH 7.3 (±0.1)

#	Name	Isotope labeling	Concentration
4	ear-domain of alpha-adaptin C	[U-99% 15N]	1.0 mM
5	sodium phosphate buffer		25 mM
6	NaCl		75 mM

Sample #3

Temperature 303 (±0.5) K, pH 7.3 (±0.1)

#	Name	Isotope labeling	Concentration
7	ear-domain of alpha-adaptin C	[U-99% 15N]	0.3 mM
8	sodium phosphate buffer		25 mM
9	NaCl		75 mM
10	Pf1 phage		2.5 mg/mL

7 2D IPAP 15N-HSQC

Instrument

Model Bruker DRX (600 MHz) Details Cryoprobe was employed

Sample #1

Temperature 303 (±0.5) K, pH 7.3 (±0.1)

#	Name	Isotope labeling	Concentration
1	ear-domain of alpha-adaptin C	[U-99% 13C; U-99% 15N]	1.0 mM
2	sodium phosphate buffer		25 mM
3	NaCl		75 mM

Sample #2

Temperature 303 (±0.5) K, pH 7.3 (±0.1)

#	Name	Isotope labeling	Concentration
4	ear-domain of alpha-adaptin C	[U-99% 15N]	1.0 mM
5	sodium phosphate buffer		25 mM
6	NaCl		75 mM

Sample #3

Temperature 303 (±0.5) K, pH 7.3 (±0.1)

#	Name	Isotope labeling	Concentration
7	ear-domain of alpha-adaptin C	[U-99% 15N]	0.3 mM
8	sodium phosphate buffer		25 mM
9	NaCl		75 mM
10	Pf1 phage		2.5 mg/mL

nullKeywords Alpha-adaptin, endocytosis, NMR assignments, residual dipolar couplings

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Found 1 Document (6.777 seconds)

BMRB: 6034

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Chemical shift reference

Related entities 1. ear-domain of alpha-adaptin C, : 1 : 8 : 5 : 3 : 30 entities Detail

Interaction partners 1. ear-domain of alpha-adaptin C, : 32 interactors Detail

Experiments performed 7 experiments Detail

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