BMRB: 6037

Solution Structure of BmKX, a novel potassium channel blocker from the Chinese Scorpion Buthus martensi Karsch

Authors

Cai, Z., Wu, J., Xu, Y., Wang, C., Chi, C., Shi, Y.

Assembly

potassium channel toxin KX

Entity

1. potassium channel toxin KX (polymer, Thiol state: all disulfide bound), 31 monomers, 3330.856 Da Detail

TPYPVNCKTD RDCVMCGLGI SCKNGYCQGC T

Formula weight

3330.856 Da

Entity Connection

disulfide 3 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS7:SG	1:CYS22:SG
2	disulfide	sing	1:CYS13:SG	1:CYS27:SG
3	disulfide	sing	1:CYS16:SG	1:CYS30:SG

Source organism

Mesobuthus martensii

Exptl. method

NMR

Refine. method

simulated annealing and energy minimization

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.8 %, Completeness: 92.9 %, Completeness (bb): 96.9 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	92.9 % (158 of 170)	92.9 % (158 of 170)
Backbone	96.9 % (62 of 64)	96.9 % (62 of 64)
Sidechain	90.6 % (96 of 106)	90.6 % (96 of 106)
Aromatic	100.0 % (8 of 8)	100.0 % (8 of 8)
Methyl	90.9 % (10 of 11)	90.9 % (10 of 11)

1. BmKX

TPYPVNCKTD RDCVMCGLGI SCKNGYCQGC T

Show sample condition

Sample

Pressure 1 atm, Temperature 300 K, pH 5.0

#	Name	Isotope labeling	Type	Concentration
1	BmKX			3 mM
2	Phosphate buffer			50 mM
3	H2O			90 %
4	D2O			10 %

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Protein Blocks Logo

Calculated from 21 models in PDB: 1RJI, Strand ID: A Detail

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Release date

2003-12-10

Citation

A novel short-chain peptide BmKX from the Chinese scorpion Buthus martensi Karsch, sequencing, gene cloning and structure determination
Wang, C.G., Cai, Z., Lu, W., Wu, J., Xu, Y., Shi, Y., Chi, C.
Toxicon. (2005), 45, 309-319, PubMed 15683869 , DOI 10.1016/j.toxicon.2004.11.014 , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Related entities 1. potassium channel toxin KX, : 1 : 2 : 2 : 7 entities Detail

Experiments performed 4 experiments Detail