BMRB: 6040

SP-B C-terminal peptide in SDS micelles

Authors

Booth, V., Waring, A.J., Walther, F.J., Keough, K.M.W.

Assembly

Pulmonary surfactant-associated protein B

Entity

1. Pulmonary surfactant-associated protein B (polymer, Thiol state: all free), 16 monomers, 1844.319 Da Detail

GRMLPQLVCR LVLRCS

Formula weight

1844.319 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 84.7 %, Completeness (bb): 78.7 % Detail

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Polymer type: polypeptide(L)

	Total	1H	15N
All	84.7 % (100 of 118)	92.2 % (94 of 102)	37.5 % (6 of 16)
Backbone	78.7 % (37 of 47)	96.9 % (31 of 32)	40.0 % (6 of 15)
Sidechain	88.7 % (63 of 71)	90.0 % (63 of 70)	0.0 % (0 of 1)
Methyl	100.0 % (12 of 12)	100.0 % (12 of 12)

1. Pulmonary surfactant-associated protein B

GRMLPQLVCR LVLRCS

Show sample condition

Sample

Pressure 1 atm, Temperature 298 K, pH 6.9

#	Name	Isotope labeling	Type	Concentration
1	Pulmonary surfactant-associated protein B	[15N]-Leu, Val		2 mM
2	sodium dodecyl sulfate	[U-2H]		200 mM
3	DSS			2 mM
4	H2O			90 %
5	D2O			10 %

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Calculated from 10 models in PDB: 1RG3, Strand ID: A Detail

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Release date

2005-01-13

Citation

NMR structures of the C-terminal segment of surfactant protein B in detergent micelles and hexafluoro-2-propanol
Booth, V., Waring, A.J., Walther, F.J., Keough, K.M.W.
Biochemistry (2004), 43, 15187-15194, PubMed 15568810 , DOI 10.1021/bi0481895 , Abstract

Related entities 1. Pulmonary surfactant-associated protein B, : 1 : 7 : 1 : 3 : 5 entities Detail

Interaction partners 1. Pulmonary surfactant-associated protein B, : 4 interactors Detail

Experiments performed 3 experiments Detail