BMRBj - BMREntryMaster

Found 1 Document (1.31 seconds)

BMRB: 6042

1RFR

NMR structure of the 30mer stemloop-D of coxsackieviral RNA

Authors: Ohlenschlager, O., Wohnert, J., Bucci, E., Seitz, S., Hafner, S., Ramachandran, R., Zell, R., Gorlach, M.
Assembly: 30mer stemloop-D of coxsackieviral RNA
Entity: 1. 30mer stemloop-D of coxsackieviral RNA (polymer, Thiol state: not present), 30 monomers, 9599.595 Da Detail
GGCACUCUGG UAUCACGGUA CCUUUGUGUC
Formula weight: 9599.595 Da
Source organism: Coxsackievirus B3
Exptl. method: NMR
Data set: coupling_constants
Release date: 2004-11-29
Citation: The structure of the stemloop D subdomain of coxsackievirus B3 cloverleaf RNA and its interaction with the proteinase 3C
Ohlenschlager, O., Wohnert, J., Bucci, E., Seitz, S., Hafner, S., Ramachandran, R., Zell, R., Gorlach, M.
Structure (2004), 12, 237-248, PubMed 14962384 , DOI 10.1016/j.str.2004.01.014 , Abstract

Related entities 1. 30mer stemloop-D of coxsackieviral RNA, : 1 : 1 entities Detail

Experiments performed 1 experiments Detail

#	Name	Isotope labeling	Concentration
1	SLD-RNA	[U-13C; U-15N]	0.9 mM
2	KH2PO4/K2HPO4		10 mM
3	KCl		40 mM
4	EDTA		0.2 mM
5	H2O		95 %
6	D2O		5 %

#	Name	Isotope labeling	Concentration
7	SLD-RNA	[U-13C; U-15N]	0.9 mM
8	KH2PO4/K2HPO4		10 mM
9	KCl		40 mM
10	EDTA		0.2 mM
11	D2O		99.99 %

#	Name	Isotope labeling	Concentration
12	SLD-RNA	[U-13C; U-15N]-G, C	1.2 mM
13	KH2PO4/K2HPO4		10 mM
14	KCl		40 mM
15	EDTA		0.2 mM
16	H2O		95 %
17	D2O		5 %

#	Name	Isotope labeling	Concentration
18	SLD-RNA	[U-13C; U-15N]-G, C	1.2 mM
19	KH2PO4/K2HPO4		10 mM
20	KCl		40 mM
21	EDTA		0.2 mM
22	D2O		99.99 %

#	Name	Isotope labeling	Concentration
23	SLD-RNA	[U-13C; U-15N]-A, U	1.2 mM
24	KH2PO4/K2HPO4		10 mM
25	KCl		40 mM
26	EDTA		0.2 mM
27	H2O		95 %
28	D2O		5 %

#	Name	Isotope labeling	Concentration
29	SLD-RNA	[U-13C; U-15N]-A, U	1.2 mM
30	KH2PO4/K2HPO4		10 mM
31	KCl		40 mM
32	EDTA		0.2 mM
33	D2O		99.99 %

#	Name	Isotope labeling	Concentration
34	SLD-RNA	[U-15N]	1.4 mM
35	KH2PO4/K2HPO4		10 mM
36	KCl		40 mM
37	EDTA		0.2 mM
38	H2O		95 %
39	D2O		5 %

#	Name	Isotope labeling	Concentration
40	SLD-RNA	[U-15N]	1.2 mM
41	KH2PO4/K2HPO4		10 mM
42	KCl		40 mM
43	EDTA		0.2 mM
44	D2O		99.99 %

nullKeywords A-form helix stems, base-paired U:U-C:U-U:U mismatch, loop with conformation similar to stable UNCG-tetraloops and U:G closing base pair

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Found 1 Document (1.31 seconds)

BMRB: 6042

Related entities 1. 30mer stemloop-D of coxsackieviral RNA, : 1 : 1 entities Detail

Experiments performed 1 experiments Detail

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