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Found 1 Document (3.008 seconds)

BMRB: 6049

1RK9

Solution Structure of Human alpha-Parvalbumin (Minimized Average Structure)

Authors

Baig, I., Bertini, I., Del Bianco, C., Gupta, Y.K., Lee, Y., Luchinat, C., Quattrone, A.

Assembly

Parvalbumin alpha

Entity

1. Parvalbumin alpha (polymer, Thiol state: not present), 110 monomers, 12058.61 Da Detail

MSMTDLLNAE DIKKAVGAFS ATDSFDHKKF FQMVGLKKKS ADDVKKVFHM LDKDKSGFIE EDELGFILKG FSPDARDLSA KETKMLMAAG DKDGDGKIGV DEFSTLVAES

2. CA (non-polymer), 40.078 Da

Total weight

12098.688 Da

Max. entity weight

12058.61 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

distance geometry; simulated annealing; molecular dynamics; torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 73.3 %, Completeness (bb): 81.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	73.3 % (927 of 1265)	79.6 % (523 of 657)	60.0 % (298 of 497)	95.5 % (106 of 111)
Backbone	81.3 % (535 of 658)	98.2 % (224 of 228)	64.5 % (207 of 321)	95.4 % (104 of 109)
Sidechain	68.9 % (488 of 708)	69.7 % (299 of 429)	67.5 % (187 of 277)	100.0 % (2 of 2)
Aromatic	21.4 % (21 of 98)	42.9 % (21 of 49)	0.0 % (0 of 49)
Methyl	81.7 % (85 of 104)	82.7 % (43 of 52)	80.8 % (42 of 52)

1. Parvalbumin alpha

MSMTDLLNAE DIKKAVGAFS ATDSFDHKKF FQMVGLKKKS ADDVKKVFHM LDKDKSGFIE EDELGFILKG FSPDARDLSA KETKMLMAAG DKDGDGKIGV DEFSTLVAES

Show sample condition

Sample

Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	Parvalbumin alpha	[U-15N; U-13C]	1.5 mM
2	CALCIUM (II) ION		3 mM
3	NaCl		100 mM
4	H2O		90 %
5	D2O		10 %

Protein Blocks Logo

Calculated from 1 models in PDB: 1RK9, Strand ID: A Detail

Release date

2004-07-29

Citation

Paramagnetism-based refinement strategy for the solution structure of human alpha-parvalbumin
Baig, I., Bertini, I., Del Bianco, C., Gupta, Y.K., Lee, Y., Luchinat, C., Quattrone, A.
Biochemistry (2004), 43, 5562-5573, PubMed 15122922 , DOI 10.1021/bi035879k , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Swiss-Prot: P20472 released on 1991-02-01
Title PRVA_HUMAN Entity Parvalbumin alpha

Related entities 1. Parvalbumin alpha, : 1 : 3 : 121 entities Detail

Interaction partners 1. Parvalbumin alpha, : 2 interactors Detail

More details for 2 interactors identified by 1 citations

Experiments performed 5 experiments Detail

1 3D 15N-separated NOESY

Instrument

Model Bruker AVANCE (500 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	Parvalbumin alpha	[U-15N; U-13C]	1.5 mM
2	CALCIUM (II) ION		3 mM
3	NaCl		100 mM
4	H2O		90 %
5	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
6	Parvalbumin alpha	[U-15N]	0.6 mM
7	CALCIUM (II) ION		3 mM
8	NaCl		100 mM
9	H2O		90 %
10	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
11	Parvalbumin alpha	[U-15N; U-13C]	1.5 mM
12	CALCIUM (II) ION		3 mM
13	NaCl		100 mM
14	H2O		90 %
15	D2O		10 %

2 3D 13C-separated NOESY

Instrument

Model Bruker AVANCE (500 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	Parvalbumin alpha	[U-15N; U-13C]	1.5 mM
2	CALCIUM (II) ION		3 mM
3	NaCl		100 mM
4	H2O		90 %
5	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
6	Parvalbumin alpha	[U-15N]	0.6 mM
7	CALCIUM (II) ION		3 mM
8	NaCl		100 mM
9	H2O		90 %
10	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
11	Parvalbumin alpha	[U-15N; U-13C]	1.5 mM
12	CALCIUM (II) ION		3 mM
13	NaCl		100 mM
14	H2O		90 %
15	D2O		10 %

3 2D NOESY

Instrument

Model Bruker AVANCE (500 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	Parvalbumin alpha	[U-15N; U-13C]	1.5 mM
2	CALCIUM (II) ION		3 mM
3	NaCl		100 mM
4	H2O		90 %
5	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
6	Parvalbumin alpha	[U-15N]	0.6 mM
7	CALCIUM (II) ION		3 mM
8	NaCl		100 mM
9	H2O		90 %
10	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
11	Parvalbumin alpha	[U-15N; U-13C]	1.5 mM
12	CALCIUM (II) ION		3 mM
13	NaCl		100 mM
14	H2O		90 %
15	D2O		10 %

4 2D TOCSY

Instrument

Model Bruker AVANCE (500 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	Parvalbumin alpha	[U-15N; U-13C]	1.5 mM
2	CALCIUM (II) ION		3 mM
3	NaCl		100 mM
4	H2O		90 %
5	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
6	Parvalbumin alpha	[U-15N]	0.6 mM
7	CALCIUM (II) ION		3 mM
8	NaCl		100 mM
9	H2O		90 %
10	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
11	Parvalbumin alpha	[U-15N; U-13C]	1.5 mM
12	CALCIUM (II) ION		3 mM
13	NaCl		100 mM
14	H2O		90 %
15	D2O		10 %

5 HNHA

Instrument

Model Bruker AVANCE (500 MHz)

Sample #1

Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	Parvalbumin alpha	[U-15N; U-13C]	1.5 mM
2	CALCIUM (II) ION		3 mM
3	NaCl		100 mM
4	H2O		90 %
5	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
6	Parvalbumin alpha	[U-15N]	0.6 mM
7	CALCIUM (II) ION		3 mM
8	NaCl		100 mM
9	H2O		90 %
10	D2O		10 %

Sample #3

Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
11	Parvalbumin alpha	[U-15N; U-13C]	1.5 mM
12	CALCIUM (II) ION		3 mM
13	NaCl		100 mM
14	H2O		90 %
15	D2O		10 %

nullKeywords calcium, parvalbumin, EF-hand, NMR, lanthanide

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Found 1 Document (3.008 seconds)

BMRB: 6049

Sample

Entries sharing articles Swiss-Prot: 1 entries Detail

Related entities 1. Parvalbumin alpha, : 1 : 3 : 121 entities Detail

Interaction partners 1. Parvalbumin alpha, : 2 interactors Detail

Experiments performed 5 experiments Detail

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