BMRB: 6058

1H, 13C and 15N Assignments for the Hypothetical Archaeglobus fulgidis protein AF2095. Northeast Structural Genomics Consortium target GR4

Authors

Powers, R., Acton, T., Huang, J.Y., Liu, J., Ma, L., Montelione, G.T., Rost, B., Xiao, R.

Assembly

Hypothetical Archaeglobus fulgidis protein AF2095

Entity

1. Hypothetical Archaeglobus fulgidis protein AF2095 (polymer, Thiol state: not present), 123 monomers, 13551.72 Da Detail

MTLKQVIVVR DDLKLSRGKL AVQVAHAAII GYLKSDSSLR RKWLDEGQKK VVLKVKSLEE LLGIKHKAES LGLVTGLVQD AGLTEVPPGT ITAVVIGPDE ERKIDKVTGN LPLLKLEHHH HHH

Formula weight

13551.72 Da

Source organism

Archaeoglobus fulgidus

Exptl. method

NMR

Refine. method

distance geometry

Data set

assigned_chemical_shifts, coupling_constants

Chem. Shift Complete

Sequence coverage: 93.5 %, Completeness: 88.6 %, Completeness (bb): 91.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	88.6 % (1281 of 1446)	88.7 % (666 of 751)	87.9 % (501 of 570)	91.2 % (114 of 125)
Backbone	91.0 % (664 of 730)	90.9 % (229 of 252)	91.1 % (327 of 359)	90.8 % (108 of 119)
Sidechain	87.0 % (721 of 829)	87.6 % (437 of 499)	85.8 % (278 of 324)	100.0 % (6 of 6)
Aromatic	40.4 % (21 of 52)	42.3 % (11 of 26)	36.0 % (9 of 25)	100.0 % (1 of 1)
Methyl	90.3 % (168 of 186)	92.5 % (86 of 93)	88.2 % (82 of 93)

1. Hypothetical Archaeglobus fulgidis protein AF2095

MTLKQVIVVR DDLKLSRGKL AVQVAHAAII GYLKSDSSLR RKWLDEGQKK VVLKVKSLEE LLGIKHKAES LGLVTGLVQD AGLTEVPPGT ITAVVIGPDE ERKIDKVTGN LPLLKLEHHH HHH

Show sample condition

Sample

Pressure 1 atm, Temperature 313 (±0.5) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Hypothetical Archaeglobus fulgidis protein AF2095	[U-99% 13C; U-99% 15N]		1.0 mM

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LACS Plot; CA

Referencing offset: -0.29 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; CB

Referencing offset: -0.29 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; HA

Referencing offset: 0.03 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; CO

Referencing offset: 2.65 ppm, Outliers: 3 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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Protein Blocks Logo

Calculated from 1 models in PDB: 1RZW, Strand ID: A Detail

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Coupling constant

70 J values in 1 lists
Pressure 1 atm, Temperature 313 (±0.5) K, pH 6.5 (±0.1) Detail

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Pressure 1 atm, Temperature 313 (±0.5) K, pH 6.5 (±0.1)

List #1 J_values_set_1

Release date

2004-09-30

Citation

(1)H, (13)C and (15)N assignments for the Archaeglobus fulgidis protein AF2095
Powers, R., Acton, T.B., Chiang, Y., Rajan, P.K., Cort, J.R., Kennedy, M.A., Liu, J., Ma, L., Rost, B., Montelione, G.T.
J. Biomol. NMR (2004), 30, 107-108, PubMed 15452442 , DOI 10.1023/B:JNMR.0000042947.19593.80 , Abstract

Related entities 1. Hypothetical Archaeglobus fulgidis protein AF2095, : 1 : 1 : 1 : 76 entities Detail

Experiments performed 6 experiments Detail