BMRB: 6059

Ras-induced losing of interaction between C1 and RBD domains of Nore1

Authors

Guiberman, E., Wohlgemuth, S., Herrmann, C., Harjes, S., Muller, K., Bayer, P.

Assembly

Nore1

Entity

1. putative Ras effector (polymer, Thiol state: free and other bound), 59 monomers, 6686.718 Da Detail

PRVLAERGEG HRFVELALRG GPGWCDLCGR EVLRQALRCA NCKFTCHSEC RSLIQLDCR

2. ZN (non-polymer), 65.409 × 2 Da

Total weight

6817.5356 Da

Max. entity weight

6686.718 Da

Entity Connection

na 6 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS25:SG	2:ZN1:ZN
2	na	sing	1:CYS28:SG	2:ZN1:ZN
3	na	sing	1:CYS50:SG	2:ZN1:ZN
4	na	sing	1:CYS39:SG	2:ZN1:ZN
5	na	sing	1:CYS42:SG	2:ZN1:ZN
6	na	sing	1:CYS58:SG	2:ZN1:ZN

Source organism

Mus musculus

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts, coupling_constants

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 67.8 %, Completeness (bb): 81.1 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	67.8 % (454 of 670)	78.8 % (278 of 353)	47.3 % (121 of 256)	90.2 % (55 of 61)
Backbone	81.1 % (284 of 350)	95.1 % (116 of 122)	66.1 % (113 of 171)	96.5 % (55 of 57)
Sidechain	59.2 % (221 of 373)	70.1 % (162 of 231)	42.8 % (59 of 138)	0.0 % (0 of 4)
Aromatic	0.0 % (0 of 40)	0.0 % (0 of 20)	0.0 % (0 of 19)	0.0 % (0 of 1)
Methyl	62.1 % (36 of 58)	96.6 % (28 of 29)	27.6 % (8 of 29)

1. putative Ras effector

PRVLAERGEG HRFVELALRG GPGWCDLCGR EVLRQALRCA NCKFTCHSEC RSLIQLDCR

Show sample condition

Sample

Temperature 300 (±1) K, pH 6.9 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	putative Ras effector	[U-95% 13C; U-90% 15N]		2.0 mM
2	pottasium phosphate puffer			20.0 mM
3	NaN3			0.5 mM
4	DTE			4 mM

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LACS Plot; CA

Referencing offset: -0.13 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; CB

Referencing offset: -0.13 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; HA

Referencing offset: -0.03 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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Protein Blocks Logo

Calculated from 21 models in PDB: 1RFH, Strand ID: A Detail

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Coupling constant

42 J values in 1 lists
Field strength (¹H) 600 MHz, Temperature 300 (±1) K, pH 6.9 (±0.2) Detail

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Temperature 300 (±1) K, pH 6.9 (±0.2)

List #1 J_values_set_1, Field strength (¹H) 600 MHz

Release date

2006-01-22

Citation

GTP-Ras disrupts the intramolecular complex of C1 and RA domains of Nore1
Harjes, E., Harjes, S., Wohlgemuth, S., Muller, K., Krieger, E., Herrmann, C., Bayer, P.
Structure (2006), 14, 881-888, PubMed 16698549 , DOI 10.1016/j.str.2006.03.008 , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Related entities 1. putative Ras effector, : 1 : 3 : 4 : 79 entities Detail

Interaction partners 1. putative Ras effector, : 17 interactors Detail

Experiments performed 6 experiments Detail