BMRB: 6062

Assignments for the Negative Regulator of Splicing from Rous Sarcoma Virus residues 907 to 929

Authors

Cabello-Villegas, J., Guiles, K., Soto, A.M., Yu, P., Beemon, K.L., Wang, Y.

Assembly

NRS23

Entity

1. NRS23 (polymer, Thiol state: not present), 23 monomers, 7413.307 Da Detail

GGGGAGUGGU UUGUAUCCUU CCC

Formula weight

7413.307 Da

Source organism

Rous sarcoma virus

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 92.7 %, Completeness (bb): 95.7 % Detail

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Polymer type: polyribonucleotide

	Total	13C	15N	31P
All	92.7 % (178 of 192)	100.0 % (153 of 153)	50.0 % (8 of 16)	73.9 % (17 of 23)
Suger, PO4	95.7 % (132 of 138)	100.0 % (115 of 115)		73.9 % (17 of 23)
Nucleobase	85.2 % (46 of 54)	100.0 % (38 of 38)	50.0 % (8 of 16)
Aromatic	85.2 % (46 of 54)	100.0 % (38 of 38)	50.0 % (8 of 16)

1. RNA stem-loop

GGGGAGUGGU UUGUAUCCUU CCC

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Sample

Temperature 298 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	RNA stem-loop	[U-13C; U-15N]		1.2 mM
2	sodium phosphate buffer			10 mM
3	NaCl			25 mM

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Release date

2004-10-28

Citation

Solution structure of the pseudo-5' splice site of a retroviral splicing suppressor
Cabello-Villegas, J., Giles, K.E., Soto, A.M., Yu, P., Mougin, A., Beemon, K.L., Wang, Y.
RNA (2004), 10, 1388-1398, PubMed 15317975 , DOI 10.1261/rna.7020804 , Abstract

Related entities 1. NRS23, : 1 : 1 entities Detail

Experiments performed 7 experiments Detail