BMRB: 6072

Solution structure of human p53 binding domain of PIAS-1

Authors

Okubo, S., Hara, F., Tsuchida, Y., Shimotakahara, S., Suzuki, S., Hatanaka, H., Yokoyama, S., Tanaka, H., Yasuda, H., Shindo, H.

Assembly

Protein inhibitor of activated STAT protein 1

Entity

1. Protein inhibitor of activated STAT protein 1 (polymer, Thiol state: not present), 65 monomers, 7450.845 Da Detail

MADSAELKQM VMSLRVSELQ VLLGYAGRNK HGRKHELLTK ALHLLKAGCS PAVQMKIKEL YRRRF

Formula weight

7450.845 Da

Source organism

Homo sapiens

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 91.2 %, Completeness (bb): 97.9 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	91.2 % (721 of 791)	86.8 % (363 of 418)	95.4 % (291 of 305)	98.5 % (67 of 68)
Backbone	97.9 % (380 of 388)	97.7 % (130 of 133)	97.9 % (187 of 191)	98.4 % (63 of 64)
Sidechain	86.4 % (401 of 464)	81.8 % (233 of 285)	93.7 % (164 of 175)	100.0 % (4 of 4)
Aromatic	55.3 % (21 of 38)	42.1 % (8 of 19)	68.4 % (13 of 19)
Methyl	100.0 % (78 of 78)	100.0 % (39 of 39)	100.0 % (39 of 39)

1. PIAS-1

MADSAELKQM VMSLRVSELQ VLLGYAGRNK HGRKHELLTK ALHLLKAGCS PAVQMKIKEL YRRRF

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Sample

Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	PIAS-1

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Protein Blocks Logo

Calculated from 20 models in PDB: 1V66, Strand ID: A Detail

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Release date

2004-08-04

Citation

NMR structure of the N-terminal domain of SUMO ligase PIAS1 and its interaction with tumor suppressor p53 and A/T-rich DNA oligomers
Okubo, S., Hara, F., Tsuchida, Y., Shimotakahara, S., Suzuki, S., Hatanaka, H., Yokoyama, S., Tanaka, H., Yasuda, H., Shindo, H.
J. Biol. Chem. (2004), 279, 31455-31461, PubMed 15133049 , DOI 10.1074/jbc.M403561200 , Abstract

Related entities 1. Protein inhibitor of activated STAT protein 1, : 1 : 5 : 1 : 1 : 24 entities Detail

Interaction partners 1. Protein inhibitor of activated STAT protein 1, : 48 interactors Detail