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Found 1 Document (1.931 seconds)

BMRB: 6073

1U3N

A functional SOD-like protein in Bacillus subtilis

Authors

Banci, L., Bertini, I., Cramaro, F., Del Conte, R., Fantoni, A., Quattrone, A., Viezzoli, M.

Assembly

Hypothetical superoxide dismutase-like protein yojM

Entity

1. Hypothetical superoxide dismutase-like protein yojM (polymer, Thiol state: all free), 162 monomers, 17229.86 Da Detail

VVETSAFGHH VQLVNREGKA VGFIEIKESD DEGLDIHISA NSLRPGASLG FHIYEKGSCV RPDFESAGGP FNPLNKEHGF NNPMGHHAGD LPNLEVGADG KVDVIMNAPD TSLKKGSKLN ILDEDGSAFI IHEQADDYLT NPSGNSGARI VCGALLGNNE KQ

Formula weight

17229.86 Da

Source organism

Bacillus subtilis

Exptl. method

NMR

Refine. method

the structure of SOD-like from bacillus subtilis was determined using triple resonance three- and bi-dimensional and homonuclear bi-dimensional NMR technique

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.5 %, Completeness: 82.7 %, Completeness (bb): 93.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	82.7 % (1470 of 1777)	79.3 % (730 of 920)	85.7 % (589 of 687)	88.8 % (151 of 170)
Backbone	93.6 % (895 of 956)	93.2 % (313 of 336)	94.6 % (441 of 466)	91.6 % (141 of 154)
Sidechain	73.7 % (710 of 963)	71.4 % (417 of 584)	78.0 % (283 of 363)	62.5 % (10 of 16)
Aromatic	42.4 % (50 of 118)	42.4 % (25 of 59)	42.4 % (25 of 59)
Methyl	92.6 % (150 of 162)	96.3 % (78 of 81)	88.9 % (72 of 81)

1. Hypothetical superoxide dismutase-like protein

VVETSAFGHH VQLVNREGKA VGFIEIKESD DEGLDIHISA NSLRPGASLG FHIYEKGSCV RPDFESAGGP FNPLNKEHGF NNPMGHHAGD LPNLEVGADG KVDVIMNAPD TSLKKGSKLN ILDEDGSAFI IHEQADDYLT NPSGNSGARI VCGALLGNNE KQ

Show sample condition

Sample #1

Pressure 1 atm, Temperature 298 (±0.1) K, pH 7.5 (±0.2)

#	Name	Isotope labeling	Concentration
1	Hypothetical superoxide dismutase-like protein	[U-15N]	1.5 mM
2	H2O		90 %
3	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 298 (±0.1) K, pH 7.5 (±0.2)

#	Name	Isotope labeling	Concentration
4	Hypothetical superoxide dismutase-like protein	[U-15N; U-13C]	1.5 mM
5	H2O		90 %
6	D2O		10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 3.03 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 3.03 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.06 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 3.36 ppm, Outliers: 5 Detail

Protein Blocks Logo

Calculated from 30 models in PDB: 1U3N, Strand ID: A Detail

Release date

2005-08-17

Citation

A prokaryotic superoxide dismutase paralog lacking two Cu ligands: from largely unstructured in solution to ordered in the crystal
Banci, L., Bertini, I., Calderone, V., Cramaro, F., Del Conte, R., Fantoni, A., Mangani, S., Quattrone, A., Viezzoli, M.SILVIA.
Proc. Natl. Acad. Sci. U. S. A. (2005), 102, 7541-7546, PubMed 15897454 , DOI 10.1073/pnas.0502450102 , Abstract

Related entities 1. Hypothetical superoxide dismutase-like protein yojM, : 1 : 2 : 1 : 192 entities Detail

Interaction partners 1. Hypothetical superoxide dismutase-like protein yojM, : 20 interactors Detail

More details for 20 interactors identified by 1 citations

Experiments performed 14 experiments Detail

1 15N-HSQC

Instrument

#1 Model Bruker Avance (500 MHz)

#2 Model Bruker Avance (600 MHz)

#3 Model Bruker Avance (700 MHz)

#4 Model Bruker Avance (800 MHz)

Sample #1

Pressure 1 atm, pH 7.5 (±0.2)

#	Name	Isotope labeling	Concentration
1	Hypothetical superoxide dismutase-like protein	[U-15N]	1.5 mM
2	H2O		90 %
3	D2O		10 %

Sample #2

Pressure 1 atm, pH 7.5 (±0.2)

#	Name	Isotope labeling	Concentration
4	Hypothetical superoxide dismutase-like protein	[U-15N; U-13C]	1.5 mM
5	H2O		90 %
6	D2O		10 %

2 15N-CLEANEX

Instrument

#1 Model Bruker Avance (500 MHz)

#2 Model Bruker Avance (600 MHz)

#3 Model Bruker Avance (700 MHz)

#4 Model Bruker Avance (800 MHz)

Sample #1

Pressure 1 atm, pH 7.5 (±0.2)

#	Name	Isotope labeling	Concentration
1	Hypothetical superoxide dismutase-like protein	[U-15N]	1.5 mM
2	H2O		90 %
3	D2O		10 %

Sample #2

Pressure 1 atm, pH 7.5 (±0.2)

#	Name	Isotope labeling	Concentration
4	Hypothetical superoxide dismutase-like protein	[U-15N; U-13C]	1.5 mM
5	H2O		90 %
6	D2O		10 %

3 HNHA

Instrument

#1 Model Bruker Avance (500 MHz)

#2 Model Bruker Avance (600 MHz)

#3 Model Bruker Avance (700 MHz)

#4 Model Bruker Avance (800 MHz)

Sample #1

Pressure 1 atm, pH 7.5 (±0.2)

#	Name	Isotope labeling	Concentration
1	Hypothetical superoxide dismutase-like protein	[U-15N]	1.5 mM
2	H2O		90 %
3	D2O		10 %

Sample #2

Pressure 1 atm, pH 7.5 (±0.2)

#	Name	Isotope labeling	Concentration
4	Hypothetical superoxide dismutase-like protein	[U-15N; U-13C]	1.5 mM
5	H2O		90 %
6	D2O		10 %

4 HNHB

Instrument

#1 Model Bruker Avance (500 MHz)

#2 Model Bruker Avance (600 MHz)

#3 Model Bruker Avance (700 MHz)

#4 Model Bruker Avance (800 MHz)

Sample #1

Pressure 1 atm, pH 7.5 (±0.2)

#	Name	Isotope labeling	Concentration
1	Hypothetical superoxide dismutase-like protein	[U-15N]	1.5 mM
2	H2O		90 %
3	D2O		10 %

Sample #2

Pressure 1 atm, pH 7.5 (±0.2)

#	Name	Isotope labeling	Concentration
4	Hypothetical superoxide dismutase-like protein	[U-15N; U-13C]	1.5 mM
5	H2O		90 %
6	D2O		10 %

5 15N-HSQC-NOESY

Instrument

#1 Model Bruker Avance (500 MHz)

#2 Model Bruker Avance (600 MHz)

#3 Model Bruker Avance (700 MHz)

#4 Model Bruker Avance (800 MHz)

Sample #1

Pressure 1 atm, pH 7.5 (±0.2)

#	Name	Isotope labeling	Concentration
1	Hypothetical superoxide dismutase-like protein	[U-15N]	1.5 mM
2	H2O		90 %
3	D2O		10 %

Sample #2

Pressure 1 atm, pH 7.5 (±0.2)

#	Name	Isotope labeling	Concentration
4	Hypothetical superoxide dismutase-like protein	[U-15N; U-13C]	1.5 mM
5	H2O		90 %
6	D2O		10 %

6 2D NOESY

Instrument

#1 Model Bruker Avance (500 MHz)

#2 Model Bruker Avance (600 MHz)

#3 Model Bruker Avance (700 MHz)

#4 Model Bruker Avance (800 MHz)

Sample #1

Pressure 1 atm, pH 7.5 (±0.2)

#	Name	Isotope labeling	Concentration
1	Hypothetical superoxide dismutase-like protein	[U-15N]	1.5 mM
2	H2O		90 %
3	D2O		10 %

Sample #2

Pressure 1 atm, pH 7.5 (±0.2)

#	Name	Isotope labeling	Concentration
4	Hypothetical superoxide dismutase-like protein	[U-15N; U-13C]	1.5 mM
5	H2O		90 %
6	D2O		10 %

7 13C-HSQC-NOESY

Instrument

#1 Model Bruker Avance (500 MHz)

#2 Model Bruker Avance (600 MHz)

#3 Model Bruker Avance (700 MHz)

#4 Model Bruker Avance (800 MHz)

Sample #1

Pressure 1 atm, pH 7.5 (±0.2)

#	Name	Isotope labeling	Concentration
1	Hypothetical superoxide dismutase-like protein	[U-15N]	1.5 mM
2	H2O		90 %
3	D2O		10 %

Sample #2

Pressure 1 atm, pH 7.5 (±0.2)

#	Name	Isotope labeling	Concentration
4	Hypothetical superoxide dismutase-like protein	[U-15N; U-13C]	1.5 mM
5	H2O		90 %
6	D2O		10 %

8 CBCA(CO)NH

Instrument

#1 Model Bruker Avance (500 MHz)

#2 Model Bruker Avance (600 MHz)

#3 Model Bruker Avance (700 MHz)

#4 Model Bruker Avance (800 MHz)

Sample #1

Pressure 1 atm, pH 7.5 (±0.2)

#	Name	Isotope labeling	Concentration
1	Hypothetical superoxide dismutase-like protein	[U-15N]	1.5 mM
2	H2O		90 %
3	D2O		10 %

Sample #2

Pressure 1 atm, pH 7.5 (±0.2)

#	Name	Isotope labeling	Concentration
4	Hypothetical superoxide dismutase-like protein	[U-15N; U-13C]	1.5 mM
5	H2O		90 %
6	D2O		10 %

9 CBCANH

Instrument

#1 Model Bruker Avance (500 MHz)

#2 Model Bruker Avance (600 MHz)

#3 Model Bruker Avance (700 MHz)

#4 Model Bruker Avance (800 MHz)

Sample #1

Pressure 1 atm, pH 7.5 (±0.2)

#	Name	Isotope labeling	Concentration
1	Hypothetical superoxide dismutase-like protein	[U-15N]	1.5 mM
2	H2O		90 %
3	D2O		10 %

Sample #2

Pressure 1 atm, pH 7.5 (±0.2)

#	Name	Isotope labeling	Concentration
4	Hypothetical superoxide dismutase-like protein	[U-15N; U-13C]	1.5 mM
5	H2O		90 %
6	D2O		10 %

10 HN(CA)CO

Instrument

#1 Model Bruker Avance (500 MHz)

#2 Model Bruker Avance (600 MHz)

#3 Model Bruker Avance (700 MHz)

#4 Model Bruker Avance (800 MHz)

Sample #1

Pressure 1 atm, pH 7.5 (±0.2)

#	Name	Isotope labeling	Concentration
1	Hypothetical superoxide dismutase-like protein	[U-15N]	1.5 mM
2	H2O		90 %
3	D2O		10 %

Sample #2

Pressure 1 atm, pH 7.5 (±0.2)

#	Name	Isotope labeling	Concentration
4	Hypothetical superoxide dismutase-like protein	[U-15N; U-13C]	1.5 mM
5	H2O		90 %
6	D2O		10 %

11 HNCO

Instrument

#1 Model Bruker Avance (500 MHz)

#2 Model Bruker Avance (600 MHz)

#3 Model Bruker Avance (700 MHz)

#4 Model Bruker Avance (800 MHz)

Sample #1

Pressure 1 atm, pH 7.5 (±0.2)

#	Name	Isotope labeling	Concentration
1	Hypothetical superoxide dismutase-like protein	[U-15N]	1.5 mM
2	H2O		90 %
3	D2O		10 %

Sample #2

Pressure 1 atm, pH 7.5 (±0.2)

#	Name	Isotope labeling	Concentration
4	Hypothetical superoxide dismutase-like protein	[U-15N; U-13C]	1.5 mM
5	H2O		90 %
6	D2O		10 %

12 13C H(C)CH-TOCSY

Instrument

#1 Model Bruker Avance (500 MHz)

#2 Model Bruker Avance (600 MHz)

#3 Model Bruker Avance (700 MHz)

#4 Model Bruker Avance (800 MHz)

Sample #1

Pressure 1 atm, pH 7.5 (±0.2)

#	Name	Isotope labeling	Concentration
1	Hypothetical superoxide dismutase-like protein	[U-15N]	1.5 mM
2	H2O		90 %
3	D2O		10 %

Sample #2

Pressure 1 atm, pH 7.5 (±0.2)

#	Name	Isotope labeling	Concentration
4	Hypothetical superoxide dismutase-like protein	[U-15N; U-13C]	1.5 mM
5	H2O		90 %
6	D2O		10 %

13 13C-(H)CCH-TOCSY

Instrument

#1 Model Bruker Avance (500 MHz)

#2 Model Bruker Avance (600 MHz)

#3 Model Bruker Avance (700 MHz)

#4 Model Bruker Avance (800 MHz)

Sample #1

Pressure 1 atm, pH 7.5 (±0.2)

#	Name	Isotope labeling	Concentration
1	Hypothetical superoxide dismutase-like protein	[U-15N]	1.5 mM
2	H2O		90 %
3	D2O		10 %

Sample #2

Pressure 1 atm, pH 7.5 (±0.2)

#	Name	Isotope labeling	Concentration
4	Hypothetical superoxide dismutase-like protein	[U-15N; U-13C]	1.5 mM
5	H2O		90 %
6	D2O		10 %

14 13C-CC(CO)NH-TOCSY

Instrument

#1 Model Bruker Avance (500 MHz)

#2 Model Bruker Avance (600 MHz)

#3 Model Bruker Avance (700 MHz)

#4 Model Bruker Avance (800 MHz)

Sample #1

Pressure 1 atm, pH 7.5 (±0.2)

#	Name	Isotope labeling	Concentration
1	Hypothetical superoxide dismutase-like protein	[U-15N]	1.5 mM
2	H2O		90 %
3	D2O		10 %

Sample #2

Pressure 1 atm, pH 7.5 (±0.2)

#	Name	Isotope labeling	Concentration
4	Hypothetical superoxide dismutase-like protein	[U-15N; U-13C]	1.5 mM
5	H2O		90 %
6	D2O		10 %

nullKeywords SOD-like, bacillus subtilis, BsSOD, solution structure, NMR

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Found 1 Document (1.931 seconds)

BMRB: 6073

Sample #1

Sample #2

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. Hypothetical superoxide dismutase-like protein yojM, : 1 : 2 : 1 : 192 entities Detail

Interaction partners 1. Hypothetical superoxide dismutase-like protein yojM, : 20 interactors Detail

Experiments performed 14 experiments Detail

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