BMRB: 6079

1H, 13C and 15N resonance assignment of the reduced form of thioredoxin h1 from Poplar, a plant CPPC active site variant

Authors

Coudevylle, N., Thureau, A., Hemmerlin, C., Gelhaye, E., Jacquot, J., Cung, M.

Assembly

thioredoxin h1 from poplar

Entity

1. thioredoxin h1 from poplar (polymer, Thiol state: not reported), 113 monomers, 12444.30 Da Detail

AEEGQVIACH TVDTWKEHFE KGKGSQKLIV VDFTASWCPP CKMIAPIFAE LAKKFPNVTF LKVDVDELKA VAEEWNVEAM PTFIFLKDGK LVDKTVGADK DGLPTLVAKH ATA

Formula weight

12444.3 Da

Source organism

Populus trichocarpa

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.3 %, Completeness: 81.6 %, Completeness (bb): 95.3 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	81.6 % (1081 of 1324)	78.6 % (535 of 681)	83.9 % (444 of 529)	89.5 % (102 of 114)
Backbone	95.3 % (635 of 666)	95.1 % (215 of 226)	95.5 % (318 of 333)	95.3 % (102 of 107)
Sidechain	71.6 % (548 of 765)	70.3 % (320 of 455)	75.2 % (228 of 303)	0.0 % (0 of 7)
Aromatic	0.0 % (0 of 118)	0.0 % (0 of 59)	0.0 % (0 of 56)	0.0 % (0 of 3)
Methyl	97.9 % (139 of 142)	97.2 % (69 of 71)	98.6 % (70 of 71)

1. thioredoxin h1 from poplar

AEEGQVIACH TVDTWKEHFE KGKGSQKLIV VDFTASWCPP CKMIAPIFAE LAKKFPNVTF LKVDVDELKA VAEEWNVEAM PTFIFLKDGK LVDKTVGADK DGLPTLVAKH ATA

Show sample condition

Sample

Temperature 298 (±1) K, pH 5.9 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	thioredoxin h1 from poplar	[U-98% 13C; U-98% 15N]		1.85 mM

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LACS Plot; CA

Referencing offset: -0.58 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; CB

Referencing offset: -0.58 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; HA

Referencing offset: 0.01 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; CO

Referencing offset: -0.38 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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Protein Blocks Logo

Calculated from 20 models in PDB: 1TI3, Strand ID: A Detail

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Release date

2004-11-28

Citation

1H, 13C and 15N resonance assignment of the reduced form of thioredoxin h1 from Poplar, a CPPC active site variant
Coudevylle, N., Thureau, A., Hemmerlin, C., Gelhaye, E., Jacquot, J., Cung, M.
J. Biomol. NMR (2004), 30, 229-230, PubMed 15557810 , DOI 10.1023/B:JNMR.0000048854.54177.1b , Abstract

Related entities 1. thioredoxin h1 from poplar, : 1 : 165 entities Detail