BMRBj - BMREntryMaster

Found 1 Document (1.738 seconds)

BMRB: 6081

2JNF

1H, 13C and 15N chemical shift assignment of fly troponin C

Authors

De Nicola, G., Biekofsky, R., Agianian, B., Bullard, B.

Assembly

troponin C

Entity

1. troponin C (polymer, Thiol state: not present), 160 monomers, 17705.32 Da Detail

GAMGDVSKLS SNQVKLLETA FRDFETPEGS GRVSTDQIGI ILEVLGIQQT KSTIRQLIDE FDPFGNGDID FDSFKIIGAR FLGEEVNPEQ MQQELREAFR LYDKEGNGYI STDVMREILA ELDETLSSED LDAMIDEIDA DGSGTVDFEE FMGVMTGGDE

Formula weight

17705.32 Da

Source organism

Lethocerus indicus

Exptl. method

NMR

Refine. method

simulated annealing, torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.4 %, Completeness: 83.9 %, Completeness (bb): 95.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	83.9 % (1508 of 1797)	81.3 % (756 of 930)	84.7 % (591 of 698)	95.3 % (161 of 169)
Backbone	95.4 % (910 of 954)	93.7 % (313 of 334)	96.3 % (446 of 463)	96.2 % (151 of 157)
Sidechain	74.5 % (735 of 986)	74.3 % (443 of 596)	74.6 % (282 of 378)	83.3 % (10 of 12)
Aromatic	51.7 % (60 of 116)	56.9 % (33 of 58)	46.6 % (27 of 58)
Methyl	84.5 % (142 of 168)	94.0 % (79 of 84)	75.0 % (63 of 84)

1. Troponin C

GAMGDVSKLS SNQVKLLETA FRDFETPEGS GRVSTDQIGI ILEVLGIQQT KSTIRQLIDE FDPFGNGDID FDSFKIIGAR FLGEEVNPEQ MQQELREAFR LYDKEGNGYI STDVMREILA ELDETLSSED LDAMIDEIDA DGSGTVDFEE FMGVMTGGDE

Show sample condition

Sample #1

Pressure 997 (±10) mbar, Temperature 298 (±1) K, pH 6.8 (±0.02)

#	Name	Isotope labeling	Concentration
1	Troponin C	[U-15N]	0.9 mM
2	KCl		100 mM
3	CaCl(2)		9 mM

Sample #2

Pressure 997 (±10) mbar, Temperature 298 (±1) K, pH 6.8 (±0.02)

#	Name	Isotope labeling	Concentration
4	Troponin C	[U-13C; U-15N]	0.97 mM
5	KCl		100 mM
6	CaCl(2)		9 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.26 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.26 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.02 ppm, Outliers: 4 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.47 ppm, Outliers: 3 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 2JNF, Strand ID: A Detail

Release date

2004-06-27

Citation 1

Assignment of the 1H, 13C, and 15N resonances of holo isoform 4 of Lethocerus indicus troponin C
De Nicola, G., Biekofsky, R., Kelly, G., Agianian, B., Qiu, F., Bullard, B., Pastore, A.
J. Biomol. NMR (2004), 29, 461-462, PubMed 15213469 , DOI 10.1023/B:JNMR.0000032558.31512.c8 , Abstract

Show more 1 citaion

Citation 2

Troponin C in different insect muscle types: identification of two isoforms in Lethocerus, Drosophila and Anopheles that are specific to asynchronous flight muscle in the adult insect
Qiu, F., Lakey, A., Agianian, B., Hutchings, A., Butcher, G.W., Labeit, S., Leonard, K., Bullard, B.
Biochem. J. (2003), 371, 811-821, PubMed 12558500 , DOI 10.1042/BJ20021814 , Abstract

Hide non-primary 1 citaion

Related entities 1. troponin C, : 1 : 290 entities Detail

Experiments performed 11 experiments Detail

1 15N HSQC

Instrument

#1 Model Varian INOVA (600 MHz)

#2 Model Varian INOVA (500 MHz)

#3 Model Varian UNITY (600 MHz)

Sample #1

Pressure 997 (±10) mbar, Temperature 298 (±1) K, pH 6.8 (±0.02)

#	Name	Isotope labeling	Concentration
1	Troponin C	[U-15N]	0.9 mM
2	KCl		100 mM
3	CaCl(2)		9 mM

Sample #2

Pressure 997 (±10) mbar, Temperature 298 (±1) K, pH 6.8 (±0.02)

#	Name	Isotope labeling	Concentration
4	Troponin C	[U-13C; U-15N]	0.97 mM
5	KCl		100 mM
6	CaCl(2)		9 mM

2 15N NOESY-HSQC

Instrument

#1 Model Varian INOVA (600 MHz)

#2 Model Varian INOVA (500 MHz)

#3 Model Varian UNITY (600 MHz)

Sample #1

Pressure 997 (±10) mbar, Temperature 298 (±1) K, pH 6.8 (±0.02)

#	Name	Isotope labeling	Concentration
1	Troponin C	[U-15N]	0.9 mM
2	KCl		100 mM
3	CaCl(2)		9 mM

Sample #2

Pressure 997 (±10) mbar, Temperature 298 (±1) K, pH 6.8 (±0.02)

#	Name	Isotope labeling	Concentration
4	Troponin C	[U-13C; U-15N]	0.97 mM
5	KCl		100 mM
6	CaCl(2)		9 mM

3 15N TOCSY-HSQC

Instrument

#1 Model Varian INOVA (600 MHz)

#2 Model Varian INOVA (500 MHz)

#3 Model Varian UNITY (600 MHz)

Sample #1

Pressure 997 (±10) mbar, Temperature 298 (±1) K, pH 6.8 (±0.02)

#	Name	Isotope labeling	Concentration
1	Troponin C	[U-15N]	0.9 mM
2	KCl		100 mM
3	CaCl(2)		9 mM

Sample #2

Pressure 997 (±10) mbar, Temperature 298 (±1) K, pH 6.8 (±0.02)

#	Name	Isotope labeling	Concentration
4	Troponin C	[U-13C; U-15N]	0.97 mM
5	KCl		100 mM
6	CaCl(2)		9 mM

4 HNCO

Instrument

#1 Model Varian INOVA (600 MHz)

#2 Model Varian INOVA (500 MHz)

#3 Model Varian UNITY (600 MHz)

Sample #1

Pressure 997 (±10) mbar, Temperature 298 (±1) K, pH 6.8 (±0.02)

#	Name	Isotope labeling	Concentration
1	Troponin C	[U-15N]	0.9 mM
2	KCl		100 mM
3	CaCl(2)		9 mM

Sample #2

Pressure 997 (±10) mbar, Temperature 298 (±1) K, pH 6.8 (±0.02)

#	Name	Isotope labeling	Concentration
4	Troponin C	[U-13C; U-15N]	0.97 mM
5	KCl		100 mM
6	CaCl(2)		9 mM

5 HNCA

Instrument

#1 Model Varian INOVA (600 MHz)

#2 Model Varian INOVA (500 MHz)

#3 Model Varian UNITY (600 MHz)

Sample #1

Pressure 997 (±10) mbar, Temperature 298 (±1) K, pH 6.8 (±0.02)

#	Name	Isotope labeling	Concentration
1	Troponin C	[U-15N]	0.9 mM
2	KCl		100 mM
3	CaCl(2)		9 mM

Sample #2

Pressure 997 (±10) mbar, Temperature 298 (±1) K, pH 6.8 (±0.02)

#	Name	Isotope labeling	Concentration
4	Troponin C	[U-13C; U-15N]	0.97 mM
5	KCl		100 mM
6	CaCl(2)		9 mM

6 CBCANH

Instrument

#1 Model Varian INOVA (600 MHz)

#2 Model Varian INOVA (500 MHz)

#3 Model Varian UNITY (600 MHz)

Sample #1

Pressure 997 (±10) mbar, Temperature 298 (±1) K, pH 6.8 (±0.02)

#	Name	Isotope labeling	Concentration
1	Troponin C	[U-15N]	0.9 mM
2	KCl		100 mM
3	CaCl(2)		9 mM

Sample #2

Pressure 997 (±10) mbar, Temperature 298 (±1) K, pH 6.8 (±0.02)

#	Name	Isotope labeling	Concentration
4	Troponin C	[U-13C; U-15N]	0.97 mM
5	KCl		100 mM
6	CaCl(2)		9 mM

7 CBCACONH

Instrument

#1 Model Varian INOVA (600 MHz)

#2 Model Varian INOVA (500 MHz)

#3 Model Varian UNITY (600 MHz)

Sample #1

Pressure 997 (±10) mbar, Temperature 298 (±1) K, pH 6.8 (±0.02)

#	Name	Isotope labeling	Concentration
1	Troponin C	[U-15N]	0.9 mM
2	KCl		100 mM
3	CaCl(2)		9 mM

Sample #2

Pressure 997 (±10) mbar, Temperature 298 (±1) K, pH 6.8 (±0.02)

#	Name	Isotope labeling	Concentration
4	Troponin C	[U-13C; U-15N]	0.97 mM
5	KCl		100 mM
6	CaCl(2)		9 mM

8 CCONH

Instrument

#1 Model Varian INOVA (600 MHz)

#2 Model Varian INOVA (500 MHz)

#3 Model Varian UNITY (600 MHz)

Sample #1

Pressure 997 (±10) mbar, Temperature 298 (±1) K, pH 6.8 (±0.02)

#	Name	Isotope labeling	Concentration
1	Troponin C	[U-15N]	0.9 mM
2	KCl		100 mM
3	CaCl(2)		9 mM

Sample #2

Pressure 997 (±10) mbar, Temperature 298 (±1) K, pH 6.8 (±0.02)

#	Name	Isotope labeling	Concentration
4	Troponin C	[U-13C; U-15N]	0.97 mM
5	KCl		100 mM
6	CaCl(2)		9 mM

9 HCCH-TOCSY

Instrument

#1 Model Varian INOVA (600 MHz)

#2 Model Varian INOVA (500 MHz)

#3 Model Varian UNITY (600 MHz)

Sample #1

Pressure 997 (±10) mbar, Temperature 298 (±1) K, pH 6.8 (±0.02)

#	Name	Isotope labeling	Concentration
1	Troponin C	[U-15N]	0.9 mM
2	KCl		100 mM
3	CaCl(2)		9 mM

Sample #2

Pressure 997 (±10) mbar, Temperature 298 (±1) K, pH 6.8 (±0.02)

#	Name	Isotope labeling	Concentration
4	Troponin C	[U-13C; U-15N]	0.97 mM
5	KCl		100 mM
6	CaCl(2)		9 mM

10 HCCONH

Instrument

#1 Model Varian INOVA (600 MHz)

#2 Model Varian INOVA (500 MHz)

#3 Model Varian UNITY (600 MHz)

Sample #1

Pressure 997 (±10) mbar, Temperature 298 (±1) K, pH 6.8 (±0.02)

#	Name	Isotope labeling	Concentration
1	Troponin C	[U-15N]	0.9 mM
2	KCl		100 mM
3	CaCl(2)		9 mM

Sample #2

Pressure 997 (±10) mbar, Temperature 298 (±1) K, pH 6.8 (±0.02)

#	Name	Isotope labeling	Concentration
4	Troponin C	[U-13C; U-15N]	0.97 mM
5	KCl		100 mM
6	CaCl(2)		9 mM

11 13C-HSQC

Instrument

#1 Model Varian INOVA (600 MHz)

#2 Model Varian INOVA (500 MHz)

#3 Model Varian UNITY (600 MHz)

Sample #1

Pressure 997 (±10) mbar, Temperature 298 (±1) K, pH 6.8 (±0.02)

#	Name	Isotope labeling	Concentration
1	Troponin C	[U-15N]	0.9 mM
2	KCl		100 mM
3	CaCl(2)		9 mM

Sample #2

Pressure 997 (±10) mbar, Temperature 298 (±1) K, pH 6.8 (±0.02)

#	Name	Isotope labeling	Concentration
4	Troponin C	[U-13C; U-15N]	0.97 mM
5	KCl		100 mM
6	CaCl(2)		9 mM

nullKeywords Trononin C, muscle, EF-hands, calcium, Lethocerus

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Found 1 Document (1.738 seconds)

BMRB: 6081

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Chemical shift reference

Related entities 1. troponin C, : 1 : 290 entities Detail

Experiments performed 11 experiments Detail

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