BMRB: 6082

Structure in solution of SFA8, a Albumin 2S from Sunflower seeds

Authors

Pantoja-Uceda, D.D., Bruix, M.M., Shewry, P.P., Santoro, J.J., Rico, M.M.

Assembly

methionine-rich 2S albumin from Sunflower seed

Entity

1. methionine-rich 2S albumin from Sunflower seed (polymer, Thiol state: all disulfide bound), 103 monomers, 12155.30 Da Detail

PYGRGRTESG CYQQMEEAEM LNHCGMYLMK NLGERSQVSP RMREEDHKQL CCMQLKNLDE KCMCPAIMMM LNEPMWIRMR DQVMSMAHNL PIECNLMSQP CQM

Formula weight

12155.3 Da

Entity Connection

disulfide 4 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS11:SG	1:CYS62:SG
2	disulfide	sing	1:CYS24:SG	1:CYS51:SG
3	disulfide	sing	1:CYS52:SG	1:CYS94:SG
4	disulfide	sing	1:CYS64:SG	1:CYS101:SG

Source organism

Helianthus annuus

Exptl. method

NMR

Refine. method

simulated annealing combined with molecular dynamics in torsion angle space followed by a final minimization.

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.0 %, Completeness: 85.2 %, Completeness (bb): 94.6 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	85.2 % (558 of 655)	85.2 % (558 of 655)
Backbone	94.6 % (194 of 205)	94.6 % (194 of 205)
Sidechain	80.9 % (364 of 450)	80.9 % (364 of 450)
Aromatic	75.0 % (18 of 24)	75.0 % (18 of 24)
Methyl	90.6 % (29 of 32)	90.6 % (29 of 32)

1. methionine-rich 2S albumin from Sunflower seed

PYGRGRTESG CYQQMEEAEM LNHCGMYLMK NLGERSQVSP RMREEDHKQL CCMQLKNLDE KCMCPAIMMM LNEPMWIRMR DQVMSMAHNL PIECNLMSQP CQM

Show sample condition

Sample

Temperature 308 (±0.1) K, pH 3.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	methionine-rich 2S albumin from Sunflower seed			1.2 mM

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Protein Blocks Logo

Calculated from 20 models in PDB: 1S6D, Strand ID: A Detail

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Release date

2004-01-22

Citation

Solution structure of a methionine-rich 2S albumin from sunflower seeds: relationship to its allergenic and emulsifying properties
Pantoja-Uceda, D.D., Shewry, P.P.R., Bruix, M.M., Tatham, A.A.S., Santoro, J.J., Rico, M.M.
Biochemistry (2004), 43, 6976-6986, PubMed 15170335 , DOI 10.1021/bi0496900 , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Related entities 1. methionine-rich 2S albumin from Sunflower seed, : 1 : 1 : 14 entities Detail

Experiments performed 3 experiments Detail