BMRB: 6085

Solution Structure of hybrid white striped bass hepcidin

Authors

Babon, J., Singh, S., Westerman, M., Pennington, M., Norton, R.

Assembly

hepcidin

Entity

1. hepcidin (polymer, Thiol state: all disulfide bound), 21 monomers, 2263.756 Da Detail

GCRFCCNCCP NMSGCGVCCR F

Formula weight

2263.756 Da

Entity Connection

disulfide 4 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS2:SG	1:CYS19:SG
2	disulfide	sing	1:CYS5:SG	1:CYS18:SG
3	disulfide	sing	1:CYS6:SG	1:CYS15:SG
4	disulfide	sing	1:CYS8:SG	1:CYS9:SG

Source organism

Morone chrysops x Morone saxatilis

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 81.8 %, Completeness (bb): 73.4 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	81.8 % (175 of 214)	95.5 % (107 of 112)	73.8 % (59 of 80)	40.9 % (9 of 22)
Backbone	73.4 % (91 of 124)	97.7 % (43 of 44)	65.0 % (39 of 60)	45.0 % (9 of 20)
Sidechain	94.4 % (102 of 108)	94.1 % (64 of 68)	100.0 % (38 of 38)	0.0 % (0 of 2)
Aromatic	100.0 % (20 of 20)	100.0 % (10 of 10)	100.0 % (10 of 10)
Methyl	100.0 % (4 of 4)	100.0 % (2 of 2)	100.0 % (2 of 2)

1. hepcidin

GCRFCCNCCP NMSGCGVCCR F

Show sample condition

Sample

Temperature 298 (±1) K, pH 4.7 (±0.3)

#	Name	Isotope labeling	Type	Concentration
1	hepcidin			3.0 ~ 4.0 mM

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Protein Blocks Logo

Calculated from 20 models in PDB: 1S6W, Strand ID: A Detail

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Release date

2004-01-28

Citation

Bass hepcidin synthesis, solution structure, antimicrobial activities and synergism, and in vivo hepatic response to bacterial infections
Lauth, X., Babon, J.J., Stannard, J.A., Singh, S., Nizet, V., Carlberg, J.M., Ostland, V.E., Pennington, M.W., Norton, R.S., Westerman, M.E.
J. Biol. Chem. (2005), 280, 9272-9282, PubMed 15546886 , DOI 10.1074/jbc.M411154200 , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Related entities 1. hepcidin, : 1 : 1 : 20 entities Detail

Experiments performed 6 experiments Detail